LOCUS       sequence-424937-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic 
            regions replaced with a BamHI/EcoRI cloning site for testing 
            different exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Schmok JC, Jain M, Street LA, Tankka AT, Schafer D, Her HL, 
            Elmsaouri S, Gosztyla ML, Boyle EA, Jagannatha P, Luo EC, Kwon EJ, 
            Jovanovic M, Yeo GW
  TITLE     Large-scale evaluation of the ability of RNA-binding proteins to 
            activate exon inclusion.
  JOURNAL   Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0.
  PUBMED    38168984
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-02014-0."
COMMENT     Sequence Label: lucMAPT-GenRep
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    878..899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(914..919,920..937,938..944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     957..979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     976..992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     976..992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1041..1162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1052..1071)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1106..1125
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1336..2130)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1447..1466)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2057..2076
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2197..2526)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2211..2346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2265..2284)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2511..2531
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2540..2968)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2650..2671)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2862..2881
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3014..3238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3227..3244
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3284..4216)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             complement(6371..8020)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFSAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             7053..7061
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(8027..8068)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8074..8083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8155..8174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8156..8174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8219..8422)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8248..8268)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8423..8802
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8974..8993
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9068..9172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9173..9241,9242..10033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9391..9410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg
     1021 tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1081 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1141 tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct
     1201 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     1261 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     1321 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     1381 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     1441 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     1501 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     1561 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     1621 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     1681 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     1741 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     1801 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     1861 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     1921 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     1981 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     2041 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     2101 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     2161 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     2221 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     2281 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     2341 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     2401 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     2461 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     2521 acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc attttttaac
     2581 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2641 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2701 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2761 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2821 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2881 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2941 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     3001 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     3061 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     3121 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     3181 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     3241 gaggctgatc agcggtttaa acttaagctt ggtaccgagc tcgtcactgc tcgttcttca
     3301 gcacgcgctc cacgaagctc ttgatgtact tacccatttc atctggagcg tcctcctggc
     3361 tgaagtggag gcccttcacc ttcacgaact cggtgttagg gaacttctta gctccctcga
     3421 caatagcgtt ggaaaagaac ccagggtcgg actcgatgaa catcttaggc agatcgtcgc
     3481 tggcccgaag gtaggcgttg tagttgcgga caatctggac gacgtcgggc ttgcctccct
     3541 taacgagagg gatctcgcga ggccaggaga gggtaggccg tctaacctcg cccttctcct
     3601 tgaatggctc caggtaggca gcgaactcct caggctccag tttccgcatg atcttgcttg
     3661 ggagcatggt ctcgacgaag aagttattct caagcaccat tttctcgccc tcttcgctct
     3721 tgatcagggc gatatcctcc tcgatgtcag gccactcgtc ccaggactcg atcacgtcca
     3781 cgacactctc agcatggacg atggccttga tcttgtcttg gtgctcgtag gagtagtgaa
     3841 aggccagaca agccccccag tcgtggccca caaagatgat tttctttgga aggttcagca
     3901 gctcgaacca agcggtgagg tacttgtagt gatccaggag gcgatatgag ccattcccgc
     3961 tcttgccgga cttacccatt ccgatcagat cagggatgat gcatctagcc acgggctcga
     4021 tgtgaggcac gacgtgcctc cacaggtagc tggaggcagc gttaccatgc agaaaaatca
     4081 cggcgttctc ggcgtgcttc tcggaatcat agtagttgat gaaggagtcc agcacgttca
     4141 tttgcttgca gcgagcccac cactgaggcc cagtgatcat gcgtttgcgt tgctcggggt
     4201 catacacctt ggaagctggt ttatgatgga tgttgcctaa tgagccacac ttggaggtca
     4261 ccttgctcag gtcaactggt ttgtagacta tttgcacctg gagatgagag aggaggagag
     4321 agaagaggaa aggcagagag agaggaagac agagagacaa aaaggataaa atgggattat
     4381 tttattaagg gtgtaactca atgagaagca gctgtgtaag cccaggccct tattggacag
     4441 tggttcccag ctgccatgag gagcacatcc tagtcagggt ggaaaaatgt gttgtcgaaa
     4501 ttctgccaac cctgccccag gggagctggg gactgtggga ctggagtgtc ttcaatccct
     4561 gcagaacaaa agagcctctg ggaaaaacag ggacccattg tcattgtgca agacccagac
     4621 atttgctcag caaacgctta agtgtaaaaa tgtcactccc tttcctgaca tgaaattgac
     4681 agatcacaat caggcatgaa gaaaaaagtg atcaatatga tgagtatgca cagcatctga
     4741 aaaatcttgc tggaaaggca ggagtggggt atctgctggg cctcaaagag gggaggcgtc
     4801 cgctcatcag aacaaacggg tcacctgtcc acagtggagc cactcaacgt caacctgccc
     4861 aggtgtcggg ggaggaggcg gcagcccagt ctcaggtgcc accaccttca gcccaacttc
     4921 caatgggcta tggggctcct ggggacaggg ctgggcatga gtggaaggag actttgatgg
     4981 gtattctcag cttcccaggc agggtccgga gagagggtcc gtcatctgcc ctattctgtc
     5041 cacacagcct gcgcaggctg ggggacccag aaatgctggg acaccccctc ctagaatatg
     5101 aggaaggggc ttctgggaac agtggaccgt gtgggggaat gtggggagcc gggctacatt
     5161 cacccagagg tcgcagccag atcctggaat tccgatcctg atccggatcc cctacttgct
     5221 cgcaaggatg cctccacttt cggacttggc agaggtagtc tggggagtga cgggaggttg
     5281 agtcacatcc caggggagct gaagggatag agggctgctc tgaaggcatc ccagaagtgt
     5341 gagtgatttc cagcgggctt tacgaagctg ggattgcagg tcctcacttc acaaaaggat
     5401 ggcatcgtga ggcctgaacc ccagagggga gcgttcacca gcctgaggtc aagtgaaaaa
     5461 tgtgttcggc cacactgacg agttagtaat aggaagttcc ccggcatctc cactcacacc
     5521 tggctagacc caccggcaca ttcccagaga actccctgcg gctgttctca agcctgccgg
     5581 gcatcagaat cacctgtgga tttctcccag aagaatcagc tgatcaaaac agaaccccca
     5641 atctccccag aatccagagg aacccaacct aagcagatgg cccacgagtg gagatgctga
     5701 cctatgggtg gcagtgtatt ctgcccagaa ggcaggtggg gaaccactgt gggtgccctg
     5761 actatgagag cctccggccg tggagacaca gccctgccat ctctgtccga ttaacagggg
     5821 ctgaaggggc aagagaagag ccagaacgtt ctctcgcctg aggagggcac tcacagtgta
     5881 gtggagagcc caataaagaa ctgaacttcc ttgaagaggg tccagagggg actggggtgt
     5941 tatgcggggg tgctcaggtg cggggagccc cacacctcca tgcacagtcc cacgactcca
     6001 cgctcaaccg cgcacctcca ggcgcagccc tacccttcca ggcacagccc tacccctcca
     6061 ggcgcagccc cccacacctc cacgcgcagc cagccactct caccttcccg cctcccggct
     6121 ggtgcttcag gttctcagtg gagccgatct tggacttgac attcttcagg tctggcatgg
     6181 gcacgggggc tgtctgcagg cggctcttgg cggaagacgg cgacttgggt ggagtacgga
     6241 ccactgccac cttcttgggc tcccgggtgg gtggggttgg aagggacggg gtgcgggagc
     6301 ggctgctggg agtgcctggg gagccggggc tgctgtagcc gctgcgatcc cctgattttg
     6361 gaggttcact cacggcgatc ttgccgcctt tcttagcctt gatcaggatc tcgcggatct
     6421 tgcgggcgtc cagcttgccg gtcaggccct tgggcacctc gtccacgaac accactccgc
     6481 cgcgcagctt cttggcggtt gtcacctggc tggccacata gtccacgatc tccttctcgg
     6541 tcatggtctt gccgtgttcc agcacgacga cggcggcagg cagctcgccg gcatcgtcgt
     6601 cgggcaggcc ggccactccg gcgtcgaaaa tgttagggtg ctgcagcagg atagactcca
     6661 gctcggctgg ggccacctgg tagcccttgt acttgatcag agacttcagg cggtccacga
     6721 tgaagaagtg ctcgtcctcg tcccagtagg caatgtcgcc agagtgcagc cagccgtcct
     6781 tgtcgatcag ggcgtttgtg gcctcagggt tattcacgta gccggacata atcatagggc
     6841 cgcgcacaca cagctcgccg cgctggttca ctcccagggt cttgccggtg tccaggtcca
     6901 ccaccttggc ctcgaagaat ggcaccacct tgcccacggc gccaggcttg tcgtcgccct
     6961 ctggggtaat cagaatggcg ctggttgtct cggtcaggcc gtagccctgg cggatgcctg
     7021 gcagatgaaa gcgcttggcc acggcttcgc ccacctcctt agacagtggg gcgccgccag
     7081 aggcaatctc gtgcaggtta gacaggtcgt acttgtcgat cagggtgctc ttagcggaga
     7141 agctgaacag ggttggcacc agcagggcag actgaatttt gtagtcttgc aggctgcgca
     7201 ggaacagctc ctcctcgaag cggtacatca gcaccacccg aaagccgcaa atcaggtagc
     7261 ccagggtggt gaacatgccg aagccgtggt ggaatggcac cacgctcaga atagcggtgt
     7321 cggggatgat ctggttgccg aaaatagggt cgcgggcgtg agagaagcgc acacaggcgg
     7381 tgcgatgagg cagggccacg cccttaggca ggccggtaga cccagagctg ttcatgatca
     7441 gggcaatggt tttgtccctg tcgaaagact ctggcacgaa gtcgtactcg ttgaagccag
     7501 gaggcagatg agatgtcacg aatgtgtaca tgctctggaa gccctggtag tcggtcttag
     7561 agtccatgat gatgatcttc tggatgatag gcagcttctt ctgcacgttc aggatcttct
     7621 gcaggccctt cttagacacg aacaccacgg taggctgaga aatgcccatg ctgttcagca
     7681 gctcgcgctc gttgtaaatg tcgttagcag gggccacggc cactccgatg aacagggcgc
     7741 ccagcactgg catgaagaac tgcagagagt tctcagagca caccacgatg cggtggttgg
     7801 tgttcaggcc gtacctcttc atggcctcgg ccaggcgcac agacatctcg aagtactcgg
     7861 cataggtgat gtccacctca atgtgggcat cggtgaaggc aatggtgcca ggcaccaggg
     7921 catacctctt catggccttg tgcagctgct cgccagcggt gccatcctcc agagggtaga
     7981 agggagcagg gcccttctta atgttcttag catcggccat gctgcccgta gaatcgagac
     8041 cgaggagagg gttagggata ggcttaccgc tgcccatggt ggctgcagat atccagcaca
     8101 gtggcggccg ctcgagtcta gagggcccgt ttaaacgcta gccagcttgg gtctccctat
     8161 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
     8221 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     8281 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     8341 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     8401 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     8461 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8521 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8581 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8641 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8701 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     8761 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     8821 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     8881 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     8941 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     9001 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     9061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10201 tt
//
