LOCUS       sequence-424955-        9332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of SRRD tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Sweeney KM, Chantarawong S, Barbieri EM, Cajka G, Liu M, Spruce L, 
            Fazelinia H, Portz B, Copley K, Lapidot T, Duhamel L, Greenwald P, 
            Saida N, Shalgi R, Shorter J, Shalem O
  TITLE     CRISPR screen for protein inclusion formation uncovers a role for 
            SRRD in the regulation of intermediate filament dynamics and 
            aggresome assembly.
  JOURNAL   PLoS Genet. 2024 Feb 5;20(2):e1011138. doi: 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb.
  PUBMED    38315730
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2024-02-5"; volume: "20(2):e1011138. doi"; pages: " 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb"
COMMENT     Sequence Label: EF1a-SRRD-HA (in pLEX_307)
FEATURES             Location/Qualifiers
     source          1..9332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2320..2346
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2365..2953
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2418..2438)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2836..2847)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3025..3258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3336..3457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3373..3392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3427..3446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3497..3632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3652..3671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3653..3671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3681..3698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3681..3697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3690..3712)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3839..4294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3926..3945)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4136..4157
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4320..4424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4425..4493,4494..5285)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4643..4662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5456..6044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5945..5964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6198..6215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6332..6353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6368..6373,6374..6391,6392..6398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6406..6422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6411..6433
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6430..6446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6430..6446
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6465..6485
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6467..6485
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6513..6739
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6740..6920
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6967..7092
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7585..7818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8003..8047
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8278..8488
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8300..8496
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8347..8482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8528..9130
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8528..8547)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9024..9044
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9173..9290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cggcaccatg gctgcggccg cagctgcggc gctggaatcc
     1321 tggcaggcgg cggctccgcg gaagaggcgc tccgcggctc gacggccgcg gcggagggag
     1381 gcggcgcccc gggggagaga ggcggcgccc cgggggagag aggcggcgcc ccggggcccc
     1441 gaggcggagt tcgagtctga cagcggagtc gtgcttcgtc gcatctggga ggctgagaag
     1501 gacctgttta tctctgattt ctggagttca gcactagaaa ccatcaatag atgtctcaca
     1561 aaacatctgg aacaactgaa ggcccctgtg gggactcttt cagacatctt tggaaacctg
     1621 catcttgact cattgccaga ggagtcagat gtggccactg attctatccc aagagagatc
     1681 ttggtcacag gaacctgcca tttgaagtgt gtgtgttacg gcattgggaa ctttgccacc
     1741 tgcatcgtag ctagaaacca gctaacgttt ttgctgcttt tgttggaaaa gtgccagatt
     1801 cccagaagtc actgttgggt atatgaccct ctgtttagcc aacttgaaat tgaagtcctt
     1861 aacacccttg gtgtgactgt tctcagtgag aacgaggaag ggaaacggag tattcgcggg
     1921 gagcctacca tcttttacat gctccattgt gggacggcct tgtacaacaa tcttttatgg
     1981 agtaactggt cagtagatgc cctttctaag atggtcatca ttgggaacag tttcaaagga
     2041 cttgaggaga ggttgttggc aaggattctg cagaaaaatt atccctacat tgcaaagatt
     2101 ttaaaaggac tggaggagct tgagtttcct cagacttcac aatacatgga catatttaat
     2161 gatacctctg tccactggtt ccctgtgcaa aagctagaac agctctccat agatatttgg
     2221 gagtttcggg aagaaccaga ttatcaggac tgtgaggacc ttgaaatcat caggaacaag
     2281 agagaagatc cttctgctac tgacggtggg tcgggcggat acccatacga tgttccagat
     2341 tacgcttaga cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2401 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2461 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2521 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2581 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2641 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2701 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2761 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2821 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2881 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2941 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3001 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3241 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3301 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     3481 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3541 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3601 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     3661 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3781 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4261 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6301 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6361 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6421 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     6481 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     6541 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6601 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     6661 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     6721 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     6781 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6841 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6901 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6961 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7021 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7081 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7141 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7201 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7261 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7321 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7381 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     7441 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     7501 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     7561 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7621 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7681 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7741 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7801 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7861 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7921 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7981 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8041 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8101 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8161 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8221 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8281 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     8341 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8401 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8461 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     8521 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     8581 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     8641 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     8701 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     8761 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     8821 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     8881 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     8941 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9001 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9061 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9121 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9181 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9241 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9301 acagggacag cagagatcca ctttggcgcc gg
//
