LOCUS       sequence-42496-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: ANKRA
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             638..655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(708..726)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      722..769
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttaact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gctctaccac acctctgtta gcaaattctt
      121 tgtctgttca ccagttggct gctcagggag agatgctcta tctggctact cgtatcgaac
      181 aagaaaatgt tatcaatcac acggatgaag aaggatttac tcctctgatg tgggctgcag
      241 cacacgggca aatagctgtg gtagagttcc tacttcagaa tggtgctgat ccccaacttt
      301 taggaaaagg tcgagaaagt gcactgtcgt tggcctgtag taaaggctac acagatattg
      361 tcaaaatgct gcttgattgt ggagttgatg taaatgaata tgattggaat ggaggaacac
      421 ctctgcttta tgctgtacat ggaaatcatg tgaaatgtgt aaagatgctc ttagaaagtg
      481 gggctgatcc aacaattgaa actgactctg gatataattc tatggatcta gctgtagccc
      541 taggctatag aagtgttcaa caggttattg agtcacattt gttgaagctg cttcaaaata
      601 tcaaggagtg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      661 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      721 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      781 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgg
//
