LOCUS       sequence-424962-       10109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of SRRD fused to V5 and APEX2 - used for 
            APEX2 proximity biotinylation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Sweeney KM, Chantarawong S, Barbieri EM, Cajka G, Liu M, Spruce L, 
            Fazelinia H, Portz B, Copley K, Lapidot T, Duhamel L, Greenwald P, 
            Saida N, Shalgi R, Shorter J, Shalem O
  TITLE     CRISPR screen for protein inclusion formation uncovers a role for 
            SRRD in the regulation of intermediate filament dynamics and 
            aggresome assembly.
  JOURNAL   PLoS Genet. 2024 Feb 5;20(2):e1011138. doi: 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb.
  PUBMED    38315730
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2024-02-5"; volume: "20(2):e1011138. doi"; pages: " 
            10.1371/journal.pgen.1011138. eCollection 2024 Feb"
COMMENT     Sequence Label: EF1a-SRRD-V5-APEX2 (in pLEX_307)
FEATURES             Location/Qualifiers
     source          1..10109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2320..2361
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2377..3123
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     misc_feature    3142..3730
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3195..3215)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3613..3624)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3802..4035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4113..4234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4150..4169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4204..4223
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4274..4409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4429..4448)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4430..4448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4458..4475)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4458..4474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4467..4489)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4616..5071
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4703..4722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4913..4934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5097..5201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5202..5270,5271..6062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5420..5439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6233..6821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6722..6741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6975..6992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7109..7130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7145..7150,7151..7168,7169..7175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7183..7199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7188..7210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7207..7223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7207..7223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7242..7262
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7244..7262
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7290..7516
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7517..7697
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7744..7869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8362..8595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8780..8824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9055..9265
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9077..9273
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9124..9259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9305..9907
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9305..9324)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9801..9821
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9950..10067
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cggcaccatg gctgcggccg cagctgcggc gctggaatcc
     1321 tggcaggcgg cggctccgcg gaagaggcgc tccgcggctc gacggccgcg gcggagggag
     1381 gcggcgcccc gtgggagaga ggcggcgccc cgggggagag aggcggcgcc ccggggcccc
     1441 gaggcggagt tcgagtctga cagcggagtc gtgcttcgtc gcatctggga ggctgagaag
     1501 gacctgttta tctctgattt ctggagttca gcactagaaa ccatcaatag atgtctcaca
     1561 aaacatctgg aacaactgaa ggcccctgtg gggactcttt cagacatctt tggaaacctg
     1621 catcttgact cattgccaga ggagtcagat gtggccactg attctatccc aagagagatc
     1681 ttggtcacag gaacctgcca tttgaagtgt gtgtgttacg gcattgggaa ctttgccacc
     1741 tgcatcgtag ctagaaacca gctaacgttt ttgctgcttt tgttggaaaa gtgccagatt
     1801 cccagaagtc actgttgggt atatgaccct ctgtttagcc aacttgaaat tgaagtcctt
     1861 aacacccttg gtgtgactgt tctcagtgag aacgaggaag ggaaacggag tattcgcggg
     1921 gagcctacca tcttttacat gctccactgt gggacggcct tgtacaacaa tcttttatgg
     1981 agtaactggt cagtagatgc cctttctaag atggtcatca ttgggaacag tttcaaagga
     2041 cttgaggaga ggttgttggc aaggattctg cagaaaaatt atccctacat tgcaaagatt
     2101 ttaaaaggac tggaggagct tgagtttcct cagacttcac aatacatgga catatttaat
     2161 gatacctctg tccactggtt ccctgtacaa aagctagaac agctctccat agatatttgg
     2221 gagtttcggg aagaaccaga ttatcaggac tgtgaggacc ttgaaatcat caggaacaag
     2281 agagaagatc cttctgctac tgacggtggg tcgggcggag gtaagcctat ccctaaccct
     2341 ctcctcggtc tcgattctac gggtgggtcg ggcggaggaa agtcttaccc aactgtgagt
     2401 gctgattacc aggacgccgt tgagaaggcg aagaagaagc tcagaggctt catcgctgag
     2461 aagagatgcg ctcctctaat gctccgtttg gcattccact ctgctggaac ctttgacaag
     2521 ggcacgaaga ccggtggacc cttcggaacc atcaagcacc ctgccgaact ggctcacagc
     2581 gctaacaacg gtcttgacat cgctgttagg cttttggagc cactcaaggc ggagttccct
     2641 attttgagct acgccgattt ctaccagttg gctggcgttg ttgccgttga ggtcacgggt
     2701 ggacctaagg ttccattcca ccctggaaga gaggacaagc ctgagccacc accagagggt
     2761 cgcttgcccg atcccactaa gggttctgac catttgagag atgtgtttgg caaagctatg
     2821 gggcttactg accaagatat cgttgctcta tctgggggtc acactattgg agctgcacac
     2881 aaggagcgtt ctggatttga gggtccctgg acctctaatc ctcttatttt cgacaactca
     2941 tacttcacgg agttgttgag tggtgagaag gaaggtctcc ttcagctacc ttctgacaag
     3001 gctcttttgt ctgaccctgt attccgccct ctcgttgaca aatatgcagc ggacgaagat
     3061 gccttctttg ctgattacgc tgaggctcac caaaagcttt ccgagcttgg gtttgctgat
     3121 gcctagacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3181 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3241 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3301 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3361 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3421 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3481 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3541 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3601 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3661 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3721 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3781 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3841 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4021 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     4081 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4141 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4201 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4261 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4381 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4441 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6361 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7081 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7141 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7201 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7261 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7321 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7381 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7441 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7501 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7681 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7741 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7801 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7861 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7921 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7981 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8041 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8101 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8161 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8221 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8281 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8341 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8401 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8461 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8521 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8581 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8641 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8701 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8761 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8821 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8881 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8941 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     9001 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9061 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9121 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9181 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9241 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9301 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9361 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9421 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9481 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9541 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9601 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9661 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9721 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9781 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9841 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9901 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9961 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    10021 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10081 gggacagcag agatccactt tggcgccgg
//
