LOCUS       sequence-424964-        2427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal empty vector with piggyBac, insulators, and a DTS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA
  TITLE     Stable integration of an optimized inducible promoter system enables
            spatiotemporal control of gene expression throughout avian 
            development.
  JOURNAL   Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 
            10.1242/bio.055343.
  PUBMED    32917762
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2020-10-6"; volume: "9(10). pii: bio.055343. doi"; 
            pages: " 10.1242/bio.055343"
COMMENT     Sequence Label: pPID2
FEATURES             Location/Qualifiers
     source          1..2427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..35
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     repeat_region   752..814
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        818..888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(889..957,958..1749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1107..1126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1832..2420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2321..2340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
       61 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      121 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acaaccgcgt
      181 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
      241 ttatattgtt atttcgtgtt ctacttacgt gataacttat tatatatata ttttcttgtt
      301 atagatatca gaggggcgga agggacgtta ggagggaggc agggaggcag ggaggcaggg
      361 aggaacggag ggagcgacag tggtaagctt ctagatcgat tataacgcgt gtcgactcga
      421 gaattctgca ggtaccatgg ccggcttaag acgtcaattg ctagcacgtg ttaacactag
      481 tacgtacatg ttggccagat ctctagatga ctcgtgctct ccctccgttc ctccctgcct
      541 ccctgcctcc ctgcctccct cctaacgtcc cttccgcccc tcttttgtta ctttatagaa
      601 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg
      661 aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata
      721 aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt
      781 taacgtacgt cacaatatga ttatctttct agggttaatt caaatatgta tccgctcatg
      841 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      901 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      961 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1021 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1081 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1141 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1201 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1261 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1321 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1381 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1441 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1501 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1561 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1621 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1681 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1741 cattggtagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1801 tctcaattag tcagcaacca aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2401 gggggcggag cctatggaaa aacttaa
//
