LOCUS       sequence-424966-       11251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYOD1-3xHA (#730)
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2399,2400..2418,2419..2441,
                     2442..2460,2461..2483,2484..2502)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2535..2573
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2570..2594
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2703..2727
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2748..2757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3720..3800
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(3823..3847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3900..3926
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4012..5223
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5060..5079
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5282..5899,5900..5905,5906..5941,5942..5944,5945..5980,
                     5981..5983,5984..6019,6020..6028)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6081..6654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6248..6265)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6475..6494
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6670..7464
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6670..7464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6724..6743)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7334..7353
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7641..8229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7694..7714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8112..8123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8304..8537
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8656..8674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8742..8846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8847..8915,8916..9707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9065..9084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9878..10466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10367..10386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10620..10637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10877..10898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10913..10918,10919..10936,10937..10943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10951..10967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10956..10978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10975..10991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10975..10991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11041..11060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt gtttaccact
     2401 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2461 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2521 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     2581 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     2641 gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt ctgcagatat
     2701 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccgcc accatggagc
     2761 tactgtcgcc accgctccgc gacgtagacc tgacggcccc cgacggctct ctctgctcct
     2821 ttgccacaac ggacgacttc tatgacgacc cgtgtttcga ctccccggac ctgcgcttct
     2881 tcgaagacct ggacccgcgc ctgatgcacg tgggcgcgct cctgaaaccc gaagagcact
     2941 cgcacttccc cgcggcggtg cacccggccc cgggcgcacg tgaggacgag catgtgcgcg
     3001 cgcccagcgg gcaccaccag gcgggccgct gcctactgtg ggcctgcaag gcgtgcaagc
     3061 gcaagaccac caacgccgac cgccgcaagg ccgccaccat gcgcgagcgg cgccgcctga
     3121 gcaaagtaaa tgaggccttt gagacactca agcgctgcac gtcgagcaat ccaaaccagc
     3181 ggttgcccaa ggtggagatc ctgcgcaacg ccatccgcta tatcgagggc ctgcaggctc
     3241 tgctgcgcga ccaggacgcc gcgccccctg gcgccgcagc cgccttctat gcgccgggcc
     3301 cgctgccccc gggccgcggc ggcgagcact acagcggcga ctccgacgcg tccagcccgc
     3361 gctccaactg ctccgacggc atgatggact acagcggccc cccgagcggc gcccggcggc
     3421 ggaactgcta cgaaggcgcc tactacaacg aggcgcccag cgaacccagg cccgggaaga
     3481 gtgcggcggt gtcgagccta gactgcctgt ccagcatcgt ggagcgcatc tccaccgaga
     3541 gccctgcggc gcccgccctc ctgctggcgg acgtgccttc tgagtcgcct ccgcgcaggc
     3601 aagaggctgc cgcccccagc gagggagaga gcagcggcga ccccacccag tcaccggacg
     3661 ccgccccgca gtgccctgcg ggtgcgaacc ccaacccgat ataccaggtg ctcctcgagt
     3721 acccatatga cgttccagac tacgcgtatc cgtacgacgt tccggattac gcttaccctt
     3781 acgatgtacc tgactacgct taatagaagg gtgggcgcgc cgacccagct ttcttgtaca
     3841 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaat
     3901 acccatacga cgtcccagac tacgcttagt aatgattaat taaactagaa attctaccgg
     3961 gtaggggagg cgcttttccc aaggcagtct ggagtaaccc acccaagatc tggcctccgc
     4021 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     4081 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     4141 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     4201 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     4261 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     4321 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     4381 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     4441 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     4501 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     4561 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     4621 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     4681 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     4741 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     4801 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     4861 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     4921 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     4981 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     5041 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     5101 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     5161 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     5221 gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag tcgaattcac
     5281 catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     5341 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     5401 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     5461 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     5521 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     5581 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     5641 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     5701 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     5761 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     5821 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     5881 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     5941 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     6001 ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc actagaggaa
     6061 ttccgcccct ctcccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc
     6121 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6181 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6241 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6301 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6361 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6421 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6481 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6541 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6601 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttac
     6661 cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6721 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6781 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6841 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6901 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6961 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7021 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7081 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     7141 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     7201 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     7261 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     7321 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     7381 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7441 cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct agatcataat
     7501 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7561 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     7621 agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     7681 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7741 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7801 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7861 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7921 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7981 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8041 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8101 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8161 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8221 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8341 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8521 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8581 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     8641 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8701 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9061 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     9121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10021 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10501 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10561 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10621 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10681 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    10741 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10801 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    10861 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10921 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10981 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    11041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    11101 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    11161 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11221 tttgttaaat atgtactaca aacttagtag t
//
