LOCUS       sequence-424984-       13281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in 
            mammilian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Klomp JE, Diehl JN, Klomp JA, Edwards AC, Yang R, Morales AJ, Taylor
            KE, Drizyte-Miller K, Bryant KL, Schaefer A, Johnson JL, Huntsman 
            EM, Yaron TM, Pierobon M, Baldelli E, Prevatte AW, Barker NK, 
            Herring LE, Petricoin EF 3rd, Graves LM, Cantley LC, Cox AD, Der CJ,
            Stalnecker CA
  TITLE     Determining the ERK-regulated phosphoproteome driving KRAS-mutant 
            cancer.
  JOURNAL   Science. 2024 Jun 7;384(6700):eadk0850. doi: 
            10.1126/science.adk0850. Epub 2024 Jun 7.
  PUBMED    38843329
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-7"; volume: "384(6700):eadk0850. doi"; pages: " 
            10.1126/science.adk0850. Epub 2024 Jun 7"
COMMENT     Sequence Label: pInducer-myc-ERK1-S170D
FEATURES             Location/Qualifiers
     source          1..13281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2375..2404
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3570..4781
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4618..4637
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4788..4821
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4828..5445,5446..5451,5452..5487,5488..5490,5491..5526,
                     5527..5529,5530..5565,5566..5574)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5623..6195
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5790..5807)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6017..6036
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6198..6800
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6697..6717
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6822..7410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6875..6895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7293..7304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7617..7850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7951..7968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7957..8181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8227..8655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8314..8333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8524..8545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8664..8684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8669..8998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8849..8984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8911..8930
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9821..9942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9991..10007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9991..10007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10004..10026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10015..10031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10039..10045,10046..10063,10064..10069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10084..10105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10222..10239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10393..10981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10473..10492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11152..11943,11944..12012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11775..11794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12013..12117)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12165..12299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12467..13100
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13147..13272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tatggagcag aagctgatct ccgaggagga
     2401 cctgggatct gggcatatgg cggcggcggc ggctcagggg ggcgggggcg gggagccccg
     2461 tagaaccgag ggggtcggcc cgggggtccc gggggaggtg gagatggtga aggggcagcc
     2521 gttcgacgtg ggcccgcgct acacgcagtt gcagtacatc ggcgagggcg cgtacggcat
     2581 ggtcagctcg gcctatgacc acgtgcgcaa gactcgcgtg gccatcaaga agatcagccc
     2641 cttcgaacat cagacctact gccagcgcac gctccgggag atccagatcc tgctgcgctt
     2701 ccgccatgag aatgtcatcg gcatccgaga cattctgcgg gcgtccaccc tggaagccat
     2761 gagagatgtc tacattgtgc aggacctgat ggagactgac ctgtacaagt tgctgaaaag
     2821 ccagcagctg agcaatgacc atatctgcta cttcctctac cagatcctgc ggggcctcaa
     2881 gtacatccac tccgccaacg tgctccaccg agatctaaag cccgacaacc tgctcatcaa
     2941 caccacctgc gaccttaaga tttgtgattt cggcctggcc cggattgccg atcctgagca
     3001 tgaccacacc ggcttcctga cggagtatgt ggctacgcgc tggtaccggg ccccagagat
     3061 catgctgaac tccaagggct ataccaagtc catcgacatc tggtctgtgg gctgcattct
     3121 ggctgagatg ctctctaacc ggcccatctt ccctggcaag cactacctgg atcagctcaa
     3181 ccacattctg ggcatcctgg gctccccatc ccaggaggac ctgaattgta tcatcaacat
     3241 gaaggcccga aactacctac agtctctgcc ctccaagacc aaggtggctt gggccaagct
     3301 tttccccaag tcagactcca aagcccttga cctgctggac cggatgttaa cctttaaccc
     3361 caataaacgg atcacagtgg aggaagcgct ggctcacccc tacctggagc agtactatga
     3421 cccgacggat gagccagtgg ccgaggagcc cttcaccttc gccatggagc tggatgacct
     3481 acctaaggag cggctgaagg agctcatctt ccaggagaca gcacgcttcc agcccggagt
     3541 gctggaggcc ccctagacgc gtaagatctg gcctccgcgc cgggttttgg cgcctcccgc
     3601 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     3661 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     3721 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     3781 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     3841 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     3901 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     3961 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
     4021 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
     4081 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
     4141 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     4201 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     4261 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     4321 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     4381 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     4441 cagggtgggg ccacctgccg gtaggtgtgt ggtaggcttt tctccgtcgc aggacgcagg
     4501 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     4561 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     4621 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     4681 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     4741 tgtccgctaa attctggccg tttttggctt ttttgttaga cggatccata acttcgtata
     4801 atgtatgcta tacgaagtta tgccaccatg tctagactgg acaagagcaa agtcataaac
     4861 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct
     4921 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     4981 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     5041 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     5101 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     5161 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     5221 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     5281 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     5341 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     5401 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     5461 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     5521 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaaataact
     5581 tcgtatagca tacattatac gaagtatggc gcgccaattc cgcccctctc cctccccccc
     5641 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5701 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5761 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5821 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5881 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5941 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6001 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6061 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6121 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6181 aaaaacacga taataccatg gccaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6241 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6301 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6361 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6421 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggctcgc
     6481 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6541 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6601 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6661 ctggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6721 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6781 cccgcaagcc cggtgcctga ttaattaagc gcgtctggaa caatcaacct ctggattaca
     6841 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6901 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6961 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7021 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7081 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7141 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7201 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7261 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7321 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7381 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
     7441 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7501 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
     7621 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7681 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7741 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7801 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7861 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7921 gccttgacat tgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7981 ctgttgtttg ccccttcccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8041 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8101 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8161 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8221 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8281 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8341 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8401 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8461 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8521 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8581 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8641 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8821 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9001 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga
     9061 actcaccgcg acgtgaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     9121 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     9181 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     9241 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     9301 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     9361 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     9421 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     9481 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     9541 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     9601 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     9661 ccccagcact cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc
     9721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9901 gcattctagt tgtggtttgt ccaaactaat caatgtatct tatcatgtct gtataccgtc
     9961 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10621 ggtcgttcgc tccaagctgg gctgtgtgca ctaacccccc gttcagcccg accgctgcgc
    10681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    11041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11161 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11221 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11281 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11341 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11401 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11461 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11521 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11581 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11641 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11701 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11761 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    12001 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    12061 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12121 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag
    12181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    12241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    12301 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    12361 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    12421 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    12481 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    12541 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    12601 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    12661 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    12721 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    12781 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    12841 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    12901 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    12961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    13021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    13081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    13141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    13201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    13261 tgcgagagcg tcagtattaa g
//
