LOCUS       sequence-424985-       13283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A
            cDNA in mammilian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Klomp JE, Diehl JN, Klomp JA, Edwards AC, Yang R, Morales AJ, Taylor
            KE, Drizyte-Miller K, Bryant KL, Schaefer A, Johnson JL, Huntsman 
            EM, Yaron TM, Pierobon M, Baldelli E, Prevatte AW, Barker NK, 
            Herring LE, Petricoin EF 3rd, Graves LM, Cantley LC, Cox AD, Der CJ,
            Stalnecker CA
  TITLE     Determining the ERK-regulated phosphoproteome driving KRAS-mutant 
            cancer.
  JOURNAL   Science. 2024 Jun 7;384(6700):eadk0850. doi: 
            10.1126/science.adk0850. Epub 2024 Jun 7.
  PUBMED    38843329
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-06-7"; volume: "384(6700):eadk0850. doi"; pages: " 
            10.1126/science.adk0850. Epub 2024 Jun 7"
COMMENT     Sequence Label: pInducer-myc-ERK1-R84S/S170D/Y280A
FEATURES             Location/Qualifiers
     source          1..13283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2110,2111..2129,2130..2152,
                     2153..2171,2172..2194,2195..2213)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2246..2284
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2281..2305
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2377..2406
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3572..4783
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4620..4639
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4790..4823
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4830..5447,5448..5453,5454..5489,5490..5492,5493..5528,
                     5529..5531,5532..5567,5568..5576)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5625..6197
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5792..5809)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6019..6038
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6200..6802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6699..6719
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6824..7412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6877..6897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7295..7306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7619..7852
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7953..7970)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7959..8183
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8229..8657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8316..8335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8526..8547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8666..8686)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8671..9000
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8851..8986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8913..8932
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9823..9944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9993..10009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9993..10009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10006..10028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10017..10033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10041..10047,10048..10065,10066..10071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10086..10107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10224..10241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10395..10983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10475..10494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11154..11945,11946..12014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11777..11796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12015..12119)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12167..12301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12469..13102
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13149..13274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tgtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2161 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2221 aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
     2281 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2341 tagaagacac cgggaccgat ccagcctacc ggtatggagc agaagctgat ctccgaggag
     2401 gacctgggat ctgggcatat ggcggcggcg gcggctcagg ggggcggggg cggggagccc
     2461 cgtagaaccg agggggtcgg cccgggggtc ccgggggagg tggagatggt gaaggggcag
     2521 ccgttcgacg tgggcccgcg ctacacgcag ttgcagtaca tcggcgaggg cgcgtacggc
     2581 atggtcagct cggcctatga ccacgtgcgc aagactcgcg tggccatcaa gaagatcagc
     2641 cccttcgaac atcagaccta ctgccagagc acgctccggg agatccagat cctgctgcgc
     2701 ttccgccatg agaatgtcat cggcatccga gacattctgc gggcgtccac cctggaagcc
     2761 atgagagatg tctacattgt gcaggacctg atggagactg acctgtacaa gttgctgaaa
     2821 agccagcagc tgagcaatga ccatatctgc tacttcctct accagatcct gcggggcctc
     2881 aagtacatcc actccgccaa cgtgctccac cgagatctaa agcccgacaa cctgctcatc
     2941 aacaccacct gcgaccttaa gatttgtgat ttcggcctgg cccggattgc cgatcctgag
     3001 catgaccaca ccggcttcct gacggagtat gtggctacgc gctggtaccg ggccccagag
     3061 atcatgctga actccaaggg ctataccaag tccatcgaca tctggtctgt gggctgcatt
     3121 ctggctgaga tgctctctaa ccggcccatc ttccctggca agcactacct ggatcagctc
     3181 aaccacattc tgggcatcct gggctcccca tcccaggagg acctgaattg tatcatcaac
     3241 atgaaggccc gaaacgccct acagtctctg ccctccaaga ccaaggtggc ttgggccaag
     3301 cttttcccca agtcagactc caaagccctt gacctgctgg accggatgtt aacctttaac
     3361 cccaataaac ggatcacagt ggaggaagcg ctggctcacc cctacctgga gcagtactat
     3421 gacccgacgg atgagccagt ggccgaggag cccttcacct tcgccatgga gctggatgac
     3481 ctacctaagg agcggctgaa ggagctcatc ttccaggaga cagcacgctt ccagcccgga
     3541 gtgctggagg ccccctagac gcgtaagatc tggcctccgc gccgggtttt ggcgcctccc
     3601 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     3661 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     3721 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     3781 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     3841 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     3901 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     3961 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     4021 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     4081 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     4141 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     4201 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     4261 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     4321 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     4381 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     4441 tgcagggtgg ggccacctgc cggtaggtgt gtggtaggct tttctccgtc gcaggacgca
     4501 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     4561 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     4621 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     4681 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     4741 attgtccgct aaattctggc cgtttttggc ttttttgtta gacggatcca taacttcgta
     4801 taatgtatgc tatacgaagt tatgccacca tgtctagact ggacaagagc aaagtcataa
     4861 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     4921 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     4981 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     5041 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     5101 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     5161 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     5221 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     5281 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     5341 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     5401 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     5461 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     5521 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaaataa
     5581 cttcgtatag catacattat acgaagtatg gcgcgccaat tccgcccctc tccctccccc
     5641 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5701 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5761 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5821 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5881 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5941 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6001 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6061 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6121 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6181 tgaaaaacac gataatacca tggccaccga gtacaagccc acggtgcgcc tcgccacccg
     6241 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     6301 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     6361 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     6421 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggctc
     6481 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6541 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6601 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6661 cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6721 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6781 gacccgcaag cccggtgcct gattaattaa gcgcgtctgg aacaatcaac ctctggatta
     6841 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6901 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6961 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7021 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7081 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7141 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7201 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7261 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7321 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7381 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     7441 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     7501 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     7561 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     7621 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7681 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7741 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7801 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7861 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7921 aggccttgac attgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     7981 atctgttgtt tgccccttcc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     8041 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     8101 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     8161 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     8221 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8281 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8341 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8401 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8461 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8521 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8581 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8641 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     8701 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     8761 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8821 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     8941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9001 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat gaaaaagcct
     9061 gaactcaccg cgacgtgacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     9121 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     9181 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     9241 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     9301 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     9361 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     9421 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     9481 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     9541 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     9601 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     9661 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt cgattccacc
     9721 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9781 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9901 ctgcattcta gttgtggttt gtccaaacta atcaatgtat cttatcatgt ctgtataccg
     9961 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    10021 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    10081 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    10141 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    10201 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10621 taggtcgttc gctccaagct gggctgtgtg cactaacccc ccgttcagcc cgaccgctgc
    10681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    11101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    11161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    11221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    11281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    12001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    12061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    12121 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
    12181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    12241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    12301 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    12361 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    12421 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
    12481 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
    12541 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
    12601 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
    12661 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
    12721 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    12781 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
    12841 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
    12901 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    12961 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    13021 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    13081 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    13141 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    13201 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    13261 ggtgcgagag cgtcagtatt aag
//
