LOCUS       sequence-424986-       13638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of YAP1-S127A/S397A cDNA in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Edwards AC, Stalnecker CA, Jean Morales A, Taylor KE, Klomp JE, 
            Klomp JA, Waters AM, Sudhakar N, Hallin J, Tang TT, Olson P, Post L,
            Christensen JG, Cox AD, Der CJ
  TITLE     TEAD Inhibition Overcomes YAP1/TAZ-driven Primary and Acquired 
            Resistance to KRASG12C Inhibitors.
  JOURNAL   Cancer Res. 2023 Nov 7. doi: 10.1158/0008-5472.CAN-23-2994.
  PUBMED    37934103
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2023 Nov 7. doi: 10.1158/0008-5472.CAN-23-2994."
COMMENT     Sequence Label: pInducer-YAP1-S127A/S397A
FEATURES             Location/Qualifiers
     source          1..13638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3884..3910
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3927..5138
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4975..4994
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5145..5178
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5185..5802,5803..5808,5809..5844,5845..5847,5848..5883,
                     5884..5886,5887..5922,5923..5931)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5980..6552
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6147..6164)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6374..6393
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6555..7157
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7054..7074
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7179..7767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7232..7252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7650..7661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7974..8207
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8308..8325)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8314..8538
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8584..9012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8671..8690)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8881..8902
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9021..9041)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9026..9355
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9206..9341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9268..9287
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10178..10299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10348..10364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10348..10364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10361..10383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10372..10388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10396..10402,10403..10420,10421..10426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10441..10462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10579..10596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10750..11338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10830..10849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11509..12300,12301..12369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12132..12151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12370..12474)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12522..12656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12824..13457
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13504..13629
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tatggatccc gggcagcagc cgccgcctca
     2401 accggccccc cagggccaag ggcagccgcc ttcgcagccc ccgcaggggc agggcccgcc
     2461 gtccggaccc gggcaaccgg cacccgcggc gacccaggcg gcgccgcagg caccccccgc
     2521 cgggcatcag atcgtgcacg tccgcgggga ctcggagacc gacctggagg cgctcttcaa
     2581 cgccgtcatg aaccccaaga cggccaacgt gccccagacc gtgcccatga ggctccggaa
     2641 gctgcccgac tccttcttca agccgccgga gcccaaatcc cactcccgac aggccagtac
     2701 tgatgcaggc actgcaggag ccctgactcc acagcatgtt cgagctcatg cctctccagc
     2761 ttctctgcag ttgggagctg tttctcctgg gacactgacc cccactggag tagtctctgg
     2821 cccagcagct acacccacag ctcagcatct tcgacagtct tcttttgaga tacctgatga
     2881 tgtacctctg ccagcaggtt gggagatggc aaagacatct tctggtcaga gatacttctt
     2941 aaatcacatc gatcagacaa caacatggca ggaccccagg aaggccatgc tgtcccagat
     3001 gaacgtcaca gcccccacca gtccaccagt gcagcagaat atgatgaact cggcttcagg
     3061 tcctcttcct gatggatggg aacaagccat gactcaggat ggagaaattt actatataaa
     3121 ccataagaac aagaccacct cttggctaga cccaaggctt gaccctcgtt ttgccatgaa
     3181 ccagagaatc agtcagagtg ctccagtgaa acagccacca cccctggctc cccagagccc
     3241 acagggaggc gtcatgggtg gcagcaactc caaccagcag caacagatgc gactgcagca
     3301 actgcagatg gagaaggaga ggctgcggct gaaacagcaa gaactgcttc ggcaggcaat
     3361 gcggaatatc aatcccagca cagcaaattc tccaaaatgt caggagttag ccctgcgtag
     3421 ccagttacca acactggagc aggatggtgg gactcaaaat ccagtgtctt ctcccgggat
     3481 gtctcaggaa ttgagaacaa tgacgaccaa tagctcagat cctttcctta acagtggcac
     3541 ctatcactct cgagatgagg ctacagacag tggactaagc atgagcagct acagtgtccc
     3601 tcgaacccca gatgacttcc tgaacagtgt ggatgagatg gatacaggtg atactatcaa
     3661 ccaaagcacc ctgccctcac agcagaaccg tttcccagac taccttgaag ccattcctgg
     3721 gacaaatgtg gaccttggaa cactggaagg agatggaatg aacatagaag gagaggagct
     3781 gatgccaagt ctgcaggaag ctttgagttc tgacatcctt aatgacatgg agtctgtttt
     3841 ggctgccacc aagctagata aagaaagctt tcttacatgg ttatatccgt atgatgttcc
     3901 ggattatgca tagacgcgta agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     3961 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     4021 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     4081 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     4141 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     4201 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     4261 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     4321 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     4381 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     4441 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     4501 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     4561 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     4621 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     4681 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     4741 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     4801 ggtggggcca cctgccggta ggtgtgtggt aggcttttct ccgtcgcagg acgcagggtt
     4861 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     4921 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     4981 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     5041 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     5101 ccgctaaatt ctggccgttt ttggcttttt tgttagacgg atccataact tcgtataatg
     5161 tatgctatac gaagttatgc caccatgtct agactggaca agagcaaagt cataaacgga
     5221 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     5281 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     5341 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     5401 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     5461 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     5521 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     5581 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     5641 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     5701 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     5761 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     5821 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     5881 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta aataacttcg
     5941 tatagcatac attatacgaa gtatggcgcg ccaattccgc ccctctccct cccccccccc
     6001 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6061 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6121 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6181 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6241 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6301 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6361 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     6421 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6481 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     6541 aacacgataa taccatggcc accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6601 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6661 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6721 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6781 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca
     6841 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6901 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6961 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg
     7021 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7081 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7141 gcaagcccgg tgcctgatta attaagcgcg tctggaacaa tcaacctctg gattacaaaa
     7201 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7261 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7321 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7381 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7441 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7501 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7561 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7621 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7681 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7741 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     7801 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7861 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7921 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     7981 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8041 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8101 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8161 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8221 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8281 ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     8341 ttgtttgccc cttccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     8401 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     8461 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     8521 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     8581 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8641 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8701 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8761 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8821 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8881 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8941 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9001 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9061 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9361 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact
     9421 caccgcgacg tgaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     9481 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     9541 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     9601 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     9661 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     9721 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     9781 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     9841 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     9901 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     9961 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    10021 cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc
    10081 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10141 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10261 ttctagttgt ggtttgtcca aactaatcaa tgtatcttat catgtctgta taccgtcgac
    10321 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10981 cgttcgctcc aagctgggct gtgtgcacta accccccgtt cagcccgacc gctgcgcctt
    11041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    11221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12481 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    12541 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    12601 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    12661 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    12721 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    12781 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    12841 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    12901 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    12961 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    13021 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    13081 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    13141 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    13201 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    13261 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    13321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    13381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    13441 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    13501 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    13561 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    13621 gagagcgtca gtattaag
//
