LOCUS       sequence-425000-       11262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, 
            T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, 
            Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic
            M, Westmeyer GG
  TITLE     Exonuclease-enhanced prime editors.
  JOURNAL   Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w.
  PUBMED    38302659
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w."
COMMENT     Sequence Label: pCAG_iPE-C
FEATURES             Location/Qualifiers
     source          1..11262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        23..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        404..681
                     /label=chicken beta-actin promoter
     intron          682..1698
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1622..1644
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1711..1730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1711..1729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1734..1743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1740..1766,1767..1769,1770..4325,4326..4328,
                     4329..5843)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5847..5873
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5853..5879
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5892..5912
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5928..5954
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5967..5993
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             8124..8144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8168..8184)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8198..8215)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8204..8428
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8500..8517)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8500..8516)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8509..8531)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8657..9112)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8794..8815)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9006..9025
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9139..9243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9244..9312,9313..10104)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9462..9481)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10275..10863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10764..10783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11017..11034
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11151..11172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11187..11192,11193..11210,11211..11217)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11225..11241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11230..11252
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(11249..11262,1..3)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(11249..11262,1..3)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaccatgagg cgcgccggat tcgacattga ttattgacta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
      421 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
      481 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg
      541 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag
      601 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
      661 aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc
      721 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
      781 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
      841 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
      901 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
      961 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     1021 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     1081 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     1141 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     1201 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     1261 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
     1321 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     1381 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
     1441 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
     1501 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
     1561 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
     1621 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
     1681 ttcttctttt tcctacagat ccttaattaa taatacgact cactataggg gccgccacca
     1741 tggacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
     1801 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
     1861 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
     1921 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
     1981 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
     2041 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
     2101 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
     2161 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
     2221 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
     2281 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
     2341 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac
     2401 ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc
     2461 tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
     2521 atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
     2581 agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
     2641 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
     2701 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
     2761 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
     2821 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
     2881 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga
     2941 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
     3001 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
     3061 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
     3121 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
     3181 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
     3241 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
     3301 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga
     3361 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
     3421 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct
     3481 ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta
     3541 tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc
     3601 tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc
     3661 acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca
     3721 ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg
     3781 atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca
     3841 gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc
     3901 acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag
     3961 tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga
     4021 tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa
     4081 tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg
     4141 tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg
     4201 atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata
     4261 tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg
     4321 acaaggcccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga
     4381 actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga
     4441 ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc
     4501 tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca
     4561 ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca
     4621 agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact
     4681 accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt
     4741 accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga
     4801 tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca
     4861 acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc
     4921 ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg
     4981 ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc
     5041 agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg
     5101 ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct
     5161 attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga
     5221 aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact
     5281 ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt
     5341 actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc
     5401 agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc
     5461 actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac
     5521 agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga
     5581 tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc
     5641 ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc
     5701 ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag
     5761 aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg
     5821 acctgtctca gctgggaggc gacgcctatc cctatgacgt gcccgattat gccagcctgg
     5881 gctccggatc ccccaagaag aaacgcaagg tggaagatgg cgagggccca gccgctaaga
     5941 gagtgaaact ggattccgga gctgctcctg ccgccaagaa gaagaaactc gacaccggtg
     6001 gacctggaag cgagcctgct acatctggct ctgagacacc tggcacctcc gagtctgcta
     6061 cacctgaatc tggacctggc gctagcaccc tgaacatcga ggacgagtac cggctgcacg
     6121 agacaagcaa agaacccgat gtgtccctgg gcagcacctg gcttagtgat ttccctcagg
     6181 cctgggccga gacaggcgga atgggacttg ctgttagaca ggcccctctg atcatccctc
     6241 tgaaggccac aagcacccct gtgtccatca agcagtaccc catgagccaa gaggcccggc
     6301 tgggaatcaa gccccacatt cagagactgc tggaccaggg catcctggtg ccttgtcaga
     6361 gcccttggaa tacccctctg ctgcccgtga agaagcccgg caccaacgat tacagacccg
     6421 tgcaggacct gcgggaagtg aacaagagag tggaagatat tcaccccacc gtgccgaatc
     6481 cttacaacct gctgtctggc ctgcctccta gccaccagtg gtacacagtg ctggacctga
     6541 aggacgcctt cttctgtctg cggctgcacc ctacaagcca gcctctgttt gcctttgagt
     6601 ggcgggaccc tgagatgggc attagcggac agctgacctg gaccagactg ccccagggct
     6661 tcaagaacag ccccacactg ttcaacgagg ccctgcatag ggacctcgcc gacttcagaa
     6721 tccagcatcc tgacctgatc ctgctccagt acgtggacga tctgctgctg gccgctacaa
     6781 gcgagctgga ttgtcagcag ggaacaagag ccctgctgca aaccctgggc aacctgggct
     6841 atagagcctc tgccaagaag gcccagattt gccagaaaca agtgaagtat ctgggctacc
     6901 tgctgaaaga gggccagcgt tggctgaccg aggccagaaa agaaaccgtg atgggccagc
     6961 ctacacctaa gacacccaga cagctgagag agttcctggg caaagccgga ttctgtcggc
     7021 tgttcatccc tggctttgcc gagatggctg cccctctgta cccactgaca aagcccggaa
     7081 ctctgttcaa ctggggccca gatcagcaga aggcctacca agagatcaag caggctctgc
     7141 tgacagcccc tgctctggga ctgcctgatc tgaccaagcc tttcgagctg ttcgtggacg
     7201 agaagcaggg ctatgccaaa ggcgtgctga cacagaagct cggcccttgg agaaggcctg
     7261 tggcctacct gagcaagaaa ctggaccctg tggctgccgg atggcctcct tgtctgagaa
     7321 tggtggccgc cattgccgtg ctgaccaagg atgccggaaa gctgacaatg ggacagcctc
     7381 tggtcattct ggcccctcat gccgtggaag ccctcgtgaa acagcctcct gatcggtggc
     7441 tgagcaacgc caggatgaca cactatcagg cactgctgct cgacaccgac agagtgcagt
     7501 ttggacctgt ggtggccctg aatcctgcca cacttctgcc tctgcctgag gaaggcctcc
     7561 agcacaattg cctggacatc ctggccgagg ctcacggcac aagacccgat ctgacagatc
     7621 agccactgcc tgacgccgac cacacctggt atacagatgg cagctctctg ctgcaagaag
     7681 gacagagaaa agccggggct gccgtgacca ccgagacaga agtgatttgg gccaaagctc
     7741 tgcccgctgg cacatctgct cagagagccg aactgatcgc cctgacacag gccctgaaaa
     7801 tggccgaggg caagaagctg aacgtctaca ccgactccag atacgccttc gccaccgctc
     7861 acatccacgg cgaaatctat cggcggagag gatggctgac cagcgagggc aaagagatta
     7921 agaacaagga cgagattctc gccctgctca aggccctgtt cctgcctaag cggctgagca
     7981 tcatccactg tccaggccac cagaagggcc actctgctga agctagaggc aacagaatgg
     8041 ccgaccaggc tgccagaaag gccgccatca cagagacacc cgataccagc acactgctga
     8101 tcgagaacag cagccctggt acccctaaga agaagcggaa agtggactga acgcgtaaat
     8161 gattgcagat ccactagttc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     8221 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8281 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8341 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8401 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     8461 agatccacta gttctagcct cgaggctaga gcggccgcca ctggccgtcg ttttacaacg
     8521 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8581 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8641 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8701 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8761 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8821 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8881 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8941 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     9001 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     9061 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     9121 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9181 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9241 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9301 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9361 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9421 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9481 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9541 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9601 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9661 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9721 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9781 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9841 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9901 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9961 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10021 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10081 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10141 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10201 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10261 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10321 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10381 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10441 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10501 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10561 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10621 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10681 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10741 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10801 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10861 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10921 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10981 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11041 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11101 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    11161 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    11221 ggaattgtga gcggataaca atttcacaca ggaaacagct at
//
