LOCUS       sequence-425005-       11155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes the enhanced traffic light reporter (eTLR) flanked by 
            homology arms for integration into the AAVS1 safe harbor locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, 
            Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic
            M, Westmeyer GG
  TITLE     Exonuclease-enhanced prime editors.
  JOURNAL   Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w.
  PUBMED    38302659
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w."
COMMENT     Sequence Label: AAVS1-eTLR
FEATURES             Location/Qualifiers
     source          1..11155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    129..150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(165..170,171..188,189..195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     208..230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     227..243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     227..243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        264..282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    317..1120
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1127..1152
                     /label=SA
                     /note="splice acceptor site"
     CDS             1176..1229
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1239..1838
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1239..1258)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1735..1755
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1876..2100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2164..2543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2545..2821
                     /label=chicken beta-actin promoter
     intron          2822..3838
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3762..3784
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        3851..3869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3874..3883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4475..4498
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4591..4647
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4657..4680
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5174..5230
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5234..5260
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5754..5810
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5814..5855
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6356..6372)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6392..6616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6687..7523
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(7550..7568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7575..7592)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7575..7591)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7584..7606)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7729..8031
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7910..7929
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8380..9174
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8380..9174
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8434..8453)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9044..9063
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9424..10215,10216..10284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10047..10066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10408..10996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10897..10916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(11150..11155,1..12)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
       61 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      121 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      181 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      241 gaccatgatt acgccaagct cagaattaac cctcactaaa gggactagtc ctgcaggttt
      301 aaacgaattc gccctttgct ttctctgacc agcattctct cccctgggcc tgtgccgctt
      361 tctgtctgca gcttgtggcc tgggtcacct ctacggctgg cccagatcct tccctgccgc
      421 ctccttcagg ttccgtcttc ctccactccc tcttcccctt gctctctgct gtgttgctgc
      481 ccaaggatgc tctttccgga gcacttcctt ctcggcgctg caccacgtga tgtcctctga
      541 gcggatcctc cccgtgtctg ggtcctctcc gggcatctct cctccctcac ccaaccccat
      601 gccgtcttca ctcgctgggt tcccttttcc ttctccttct ggggcctgtg ccatctctcg
      661 tttcttagga tggccttctc cgacggatgt ctcccttgcg tcccgcctcc ccttcttgta
      721 ggcctgcatc atcaccgttt ttctggacaa ccccaaagta ccccgtctcc ctggctttag
      781 ccacctctcc atcctcttgc tttctttgcc tggacacccc gttctcctgt ggattcgggt
      841 cacctctcac tcctttcatt tgggcagctc ccctaccccc cttacctctc tagtctgtgc
      901 tagctcttcc agccccctgt catggcatct tccaggggtc cgagagctca gctagtcttc
      961 ttcctccaac ccgggcccct atgtccactt caggacagca tgtttgctgc ctccagggat
     1021 cctgtgtccc cgagctggga ccaccttata ttcccagggc cggttaatgt ggctctggtt
     1081 ctgggtactt ttatctgtcc cctccacccc acagtggggc aagcttctga cctcttctct
     1141 tcctcccaca gggcctcgag agatctggca gcggagaggg cagaggaagt cttctaacat
     1201 gcggtgacgt ggaggagaat cccggcccta ggctcgagat gaccgagtac aagcccacgg
     1261 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     1321 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     1381 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     1441 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     1501 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     1561 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     1621 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     1681 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     1741 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     1801 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc tagagggccc gtttaaaccc
     1861 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     1921 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     1981 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2041 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2101 gtcgacggta tcgataagct tgatatcgaa ttcgagctcg gtacccgggg gcgcgccgga
     2161 ttcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2221 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2281 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2341 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2401 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2461 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2521 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     2581 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     2641 cgatgggggc gggggggggg gggggggcgc gccaggcggg gcggggcggg gcgaggggcg
     2701 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     2761 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     2821 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     2881 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     2941 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     3001 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     3061 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     3121 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     3181 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     3241 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     3301 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     3361 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     3421 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     3481 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     3541 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     3601 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     3661 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     3721 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     3781 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagat
     3841 ccttaattaa taatacgact cactataggg gccgccacca tggtttctaa aggcgaggcc
     3901 gtgatcaaag agttcatgag gttcaaggtg cacatggaag gcagcatgaa cggccacgag
     3961 ttcgagatcg aaggcgaagg cgagggcaga ccttacgagg gaactcagac cgccaagctg
     4021 aaagtgacca aaggcggccc tctgcctttc agctgggaca tcctgtctcc acagtttatg
     4081 tacggcagca gggccttcat caagcacccc gctgacatcc ccgactacta caagcagtct
     4141 ttcccagagg gcttcaagtg ggagagagtg atgaacttcg aggacggcgg agccgtgact
     4201 gtgacacagg acacatctct ggaagatggg accctgatct acaaagtgaa gctgcggggc
     4261 acaaacttcc ctccagacgg ccctgtgatg cagaaaaaga caatgggctg ggaagccagc
     4321 accgagagac tgtaccctga agatggcgtg ctgaagggcg acatcaagat ggccctgaga
     4381 ctgaaggacg gcggcagata cctggccgac ttcaagacca cctacaaggc caagaaacct
     4441 gtgcagatgc ctggcgccta caacgtggac agaaagctgg acatcacctc ccacaacgaa
     4501 gactgaccgg gcgcttccgc ctccggttcc ggcagcggat ccgagacacc tggcacaagc
     4561 gagtctgcca caccagagtc tggaagcggc gccacaaact tcagcctgct caaacaggct
     4621 ggcgacgtgg aagagaaccc tggacctggc tctgccgact acaaggacga cgacgacaaa
     4681 ggctctggca gcgaaacccc tggcacatcc gaaagcgcta caccagaaag cggaggcggc
     4741 ggactgctgg aaaagttcgt cggcacctgg aagatcgccg actctcacaa cttcggcgag
     4801 tacctcaagg ccatcggcgc tccaaaagag ttgtcagacg gaggcgacgc caccacacct
     4861 acactgtaca tcagccagaa agacggcgac aaaatgtccg tcaagatcga gaacggccct
     4921 cctaccttcc tggacaccca agtcaagttc aagctgggcg aggagttcga cgagtttcca
     4981 agcgacagac ggaagggagt caagtccgtg gtcaacctcg tgggagagaa gctggtgtac
     5041 gtgcagaagt gggacggcaa agagacaacc tacgtcagag agatcaagga cggcaagctg
     5101 gtggtcaccc tcactatggg cgacgtggtg gccgtgcgga gctacagaag ggccacagag
     5161 tgaaggcagc ggcgctacca acttttcact gctcaagcag gccggggacg tcgaggaaaa
     5221 tcctggacca gcttacccct acgacgtgcc agattacgct ggctccggca gcgaaacacc
     5281 aggcaccagc gagtctgcta ccccagaatc aggtggcgga ggcctgctcg agaagtttgt
     5341 tggaacatgg aagattgccg acagtcacaa ttttggggag tatctcaagg ctatcggagc
     5401 cccaaaagaa ctgtccgacg gcggcgacgc tacaacccca actctgtaca tctctcaaaa
     5461 ggacggggac aagatgtctg ttaagatcga aaacgggccg ccaacattcc tggatacgca
     5521 agtcaaattc aaactcggcg aagagtttga cgagttccca tccgacagaa gaaagggcgt
     5581 caagagcgtg gtcaatctcg tgggcgaaaa actggtctac gtccaaaagt gggacgggaa
     5641 agaaaccaca tacgtgcggg aaatcaagga cggaaagctc gtggtcacac tcacaatggg
     5701 cgacgtcgtg gctgtgcggt cttacagacg cgctaccgaa tgacggatct ggcgctacca
     5761 atttcagcct tctcaagcaa gcaggcgacg tggaggaaaa cccagggcct gctggaaagc
     5821 ccattccaaa tcctctgctg ggcctcgaca gcaccggcag tggttccgaa actcccggaa
     5881 caagcgagag cgccacacca gaatctggcg gtggtggact gctggagaaa tttgtcggta
     5941 cttggaaaat cgccgacagc cacaacttcg gagaatacct caaagctatt ggggcaccca
     6001 aagagctttc cgacggcggg gacgcaacta ctcccacact gtatatttcc caaaaggatg
     6061 gggacaaaat gtccgtcaaa atcgagaatg gaccgccgac ttttctcgac acacaagtca
     6121 agttcaaatt gggagaagag ttcgatgaat ttcccagcga ccgccgcaaa ggcgtcaaat
     6181 ctgtggtcaa ccttgtcggg gaaaagctcg tttacgttca aaaatgggac ggaaaagaaa
     6241 cgacctatgt ccgcgaaatc aaagacggga agcttgttgt tactcttacg atgggcgatg
     6301 tggtcgccgt caggtcttat cggagagcca cagagtgaac gcgtaaatga ttgcagatcc
     6361 actagttcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6481 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6541 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6601 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag atccactagt
     6661 tctagcctcg aggctagagc ggccgcacta gggacaggat tggtgacaga aaagccccat
     6721 ccttaggcct cctccttcct agtctcctga tattgggtct aacccccacc tcctgttagg
     6781 cagattcctt atctggtgac acacccccat ttcctggagc catctctctc cttgccagaa
     6841 cctctaaggt ttgcttacga tggagccaga gaggatcctg ggagggagag cttggcaggg
     6901 ggtgggaggg aaggggggga tgcgtgacct gcccggttct cagtggccac cctgcgctac
     6961 cctctcccag aacctgagct gctctgacgc ggctgtctgg tgcgtttcac tgatcctggt
     7021 gctgcagctt ccttacactt cccaagagga gaagcagttt ggaaaaacaa aatcagaata
     7081 agttggtcct gagttctaac tttggctctt cacctttcta gtccccaatt tatattgttc
     7141 ctccgtgcgt cagttttacc tgtgagataa ggccagtagc cagccccgtc ctggcagggc
     7201 tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt gtgagaatgg tgcgtcctag
     7261 gtgttcacca ggtcgtggcc gcctctactc cctttctctt tctccatcct tctttcctta
     7321 aagagtcccc agtgctatct gggacatatt cctccgccca gagcagggtc ccgcttccct
     7381 aaggccctgc tctgggcttc tgggtttgag tccttggcaa gcccaggaga ggcgctcagg
     7441 cttccctgtc ccccttcctc gtccaccatc tcatgcccct ggctctcctg ccccttccct
     7501 acaggggttc ctggctctgc tctaagggcg gccgctaaat tcaattcgcc ctatagtgag
     7561 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7621 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7681 gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt
     7741 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     7801 gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     7861 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     7921 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     7981 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     8041 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag
     8101 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
     8161 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
     8221 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
     8281 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     8341 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     8401 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8461 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8521 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     8581 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8641 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8701 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8761 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8821 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     8881 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     8941 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     9001 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     9061 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     9121 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt
     9181 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9241 cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9301 aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca cctagatcct
     9361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9961 ttctgtgact ggtgaatact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10321 gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    11041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    11101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgag
//
