LOCUS       sequence-425010-       13370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of TAZ wild type cDNA in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Edwards AC, Stalnecker CA, Jean Morales A, Taylor KE, Klomp JE, 
            Klomp JA, Waters AM, Sudhakar N, Hallin J, Tang TT, Olson P, Post L,
            Christensen JG, Cox AD, Der CJ
  TITLE     TEAD Inhibition Overcomes YAP1/TAZ-driven Primary and Acquired 
            Resistance to KRASG12C Inhibitors.
  JOURNAL   Cancer Res. 2023 Nov 7. doi: 10.1158/0008-5472.CAN-23-2994.
  PUBMED    37934103
REFERENCE   2  (bases 1 to 13370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2023 Nov 7. doi: 10.1158/0008-5472.CAN-23-2994."
COMMENT     Sequence Label: pInducer-TAZ
FEATURES             Location/Qualifiers
     source          1..13370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2110,2111..2129,2130..2152,
                     2153..2171,2172..2194,2195..2213)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2246..2284
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2281..2305
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2380..2406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2410..2436
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3659..4870
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4707..4726
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4877..4910
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4917..5534,5535..5540,5541..5576,5577..5579,5580..5615,
                     5616..5618,5619..5654,5655..5663)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5712..6284
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5879..5896)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6106..6125
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6287..6889
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6786..6806
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6911..7499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6964..6984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7382..7393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7706..7939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8040..8057)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8046..8270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8316..8744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8403..8422)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8613..8634
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8753..8773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8758..9087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8938..9073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9000..9019
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9910..10031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10080..10096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10080..10096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10093..10115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10104..10120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10128..10134,10135..10152,10153..10158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10173..10194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10311..10328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10482..11070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10562..10581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11241..12032,12033..12101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11864..11883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12102..12206)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12254..12388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12556..13189
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13236..13361
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tgtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2161 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2221 aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
     2281 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2341 tagaagacac cgggaccgat ccagcctacc ggtatggcct acccatacga tgttcctgac
     2401 tatgcgggct atccctatga cgtcccggac tatgcaggat ccatgaatcc ggcctcggcg
     2461 ccccctccgc tcccgccgcc tgggcagcaa gtgatccacg tcacgcagga cctagacaca
     2521 gacctcgaag ccctcttcaa ctctgtcatg aatccgaagc ctagctcgtg gcggaagaag
     2581 atcctgccgg agtctttctt taaggagcct gattcgggct cgcactcgcg ccagtccagc
     2641 accgactcgt cgggcggcca cccggggcct cgactggctg ggggtgccca acatgtccgc
     2701 tcgcactcgt cgcccgcgtc cctgcagctg ggcaccggcg cgggtgctgc gggtagcccc
     2761 gcgcagcagc acgcgcacct ccgccagcag tcctacgacg tgaccgacga gctgccactg
     2821 cccccgggct gggagatgac cttcacggcc actggccaga ggtacttcct caatcacata
     2881 gaaaaaatca ccacatggca agaccctagg aaggcgatga atcagcctct gaatcatatg
     2941 aacctccacc ctgccgtcag ttccacacca gtgcctcaga ggtccatggc agtatcccag
     3001 ccaaatctcg tgatgaatca ccaacaccag cagcagatgg cccccagtac cctgagccag
     3061 cagaaccacc ccactcagaa cccacccgca gggctcatga gtatgcccaa tgcgctgacc
     3121 actcagcagc agcagcagca gaaactgcgg cttcagagaa tccagatgga gagagaaagg
     3181 attcgaatgc gccaagagga gctcatgagg caggaagctg ccctctgtcg acagctcccc
     3241 atggaagctg agactcttgc cccagttcag gctgctgtca acccacccac gatgacccca
     3301 gacatgagat ccatcactaa taatagctca gatcctttcc tcaatggagg gccatatcat
     3361 tcgagggagc agagcactga cagtggcctg gggttagggt gctacagtgt ccccacaact
     3421 ccggaggact tcctcagcaa tgtggatgag atggatacag gagaaaacgc aggacaaaca
     3481 cccatgaaca tcaatcccca acagacccgt ttccctgatt tccttgactg tcttccagga
     3541 acaaacgttg acttaggaac tttggaatct gaagacctga tccccctctt caatgatgta
     3601 gagtctgctc tgaacaaaag tgagcccttt ctaacctggc tgtaaacgcg taagatctgg
     3661 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     3721 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     3781 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     3841 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     3901 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     3961 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     4021 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     4081 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     4141 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     4201 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     4261 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     4321 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     4381 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     4441 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     4501 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgtg
     4561 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     4621 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     4681 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     4741 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     4801 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     4861 tttgttagac ggatccataa cttcgtataa tgtatgctat acgaagttat gccaccatgt
     4921 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     4981 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     5041 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     5101 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     5161 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     5221 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     5281 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     5341 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     5401 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     5461 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5521 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5581 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5641 accttgacat gctccccggg taaataactt cgtatagcat acattatacg aagtatggcg
     5701 cgccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5761 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5821 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5881 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5941 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     6001 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     6061 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6121 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     6181 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6241 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg ccaccgagta
     6301 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6361 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6421 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6481 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6541 gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc
     6601 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6661 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6721 gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc
     6781 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6841 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat taattaagcg
     6901 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6961 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7021 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7081 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7141 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7201 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7261 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7321 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7381 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7441 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7501 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     7561 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7621 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7681 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     7741 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7801 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7861 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7921 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7981 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc
     8041 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc cccttccccg tgccttcctt
     8101 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     8161 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8221 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     8281 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     8341 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8401 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8461 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8521 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8581 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     8641 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     8701 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     8761 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8821 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8881 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9001 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     9061 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     9121 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca aggaatcggt
     9181 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     9241 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     9301 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     9361 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     9421 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     9481 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     9541 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     9601 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     9661 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     9721 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     9781 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9841 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9901 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9961 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactaatc
    10021 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    10081 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10141 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10201 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10261 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    10321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10741 taaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10921 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    11101 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    11161 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    11221 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    11281 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    11341 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11401 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11461 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11521 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11581 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11641 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11701 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11761 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11821 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11881 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11941 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12001 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12061 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    12121 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    12181 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    12241 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    12301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    12361 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    12421 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    12481 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    12541 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
    12601 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
    12661 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
    12721 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
    12781 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
    12841 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
    12901 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
    12961 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
    13021 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    13081 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    13141 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    13201 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    13261 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    13321 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
//
