LOCUS       sequence-425013-        5100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA 
            cloning cassette, facilitating high-fidelity cytosine base editing 
            in Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
  TITLE     Engineered cytosine base editor enabling broad-scope and 
            high-fidelity gene editing in Streptomyces.
  JOURNAL   Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
  PUBMED    38971862
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-7"; volume: "15(1):5687. doi"; pages: " 
            10.1038/s41467-024-49987-3"
COMMENT     Sequence Label: pSCBE3-HF1
FEATURES             Location/Qualifiers
     source          1..5100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..687
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             4849..5100
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atgtcgtccg agaccggccc ggtggccgtg gacccgaccc tgcgtcgccg catcgagccg
       61 cacgagttcg aggtgttctt cgacccgcgg gagctgcgca aggagacctg cctgctgtac
      121 gagatcaact ggggcggccg gcactcgatc tggcgccaca cctcgcagaa caccaacaag
      181 cacgtcgagg tgaacttcat cgagaagttc accaccgagc ggtacttctg cccgaacacc
      241 cgctgctcga tcacctggtt cctgtcctgg tccccgtgcg gcgagtgctc ccgggcgatc
      301 accgagttcc tgtcccgcta cccgcacgtc accctgttca tctacatcgc ccggctgtac
      361 caccacgccg acccgcggaa ccggcagggc ctgcgcgacc tgatctcctc cggcgtgacc
      421 atccagatca tgaccgagca ggagtccggc tactgctggc ggaacttcgt caactactcg
      481 ccgtccaacg aggcccactg gccgcggtac ccgcacctgt gggtgcgcct gtacgtcctg
      541 gagctgtact gcatcatcct gggcctgccg ccgtgcctga acatcctgcg tcgcaagcag
      601 ccgcagctga ccttcttcac catcgccctg cagtcctgcc actaccagcg cctgccgccg
      661 cacatcctgt gggcgaccgg cctgaagtcc ggctcggaga ccccgggcac ctcggagtcc
      721 gccaccccgg agtccgacaa gaagtacagc atcggcctgg ccatcggcac caacagcgtg
      781 ggctgggcgg tcatcaccga cgagtacaag gtcccctcca agaagttcaa ggtcctgggc
      841 aacaccgacc ggcactcgat caagaagaac ctgatcggcg ccctgctctt cgacagcggc
      901 gagaccgccg aggcgacccg cctgaagcgg accgcgcgcc ggcgctacac ccggcgcaag
      961 aaccgcatct gctacctcca ggaaatcttc tccaacgaga tggccaaggt cgacgactcg
     1021 ttcttccacc ggctcgagga gagcttcctg gtggaggagg acaagaagca cgagcgccac
     1081 ccgatcttcg gcaacatcgt cgacgaggtg gcctaccacg agaagtaccc caccatctac
     1141 cacctccgca agaagctggt cgactcgacc gacaaggcgg acctgcggct catctacctg
     1201 gccctcgcgc acatgatcaa gttccgcggc cacttcctca tcgagggcga cctgaacccg
     1261 gacaactccg acgtcgacaa gctcttcatc cagctggtgc agacctacaa ccagctgttc
     1321 gaggagaacc ccatcaacgc cagcggcgtc gacgccaagg cgatcctctc cgcgcgcctg
     1381 agcaagtccc ggcgcctgga gaacctcatc gcccagctgc cgggcgagaa gaagaacggc
     1441 ctcttcggca acctgatcgc gctgtcgctc ggcctgaccc ccaacttcaa gagcaacttc
     1501 gacctggccg aggacgcgaa gctccagctg tccaaggaca cctacgacga cgacctggac
     1561 aacctgctcg cccagatcgg cgaccagtac gcggacctct tcctggccgc gaagaacctc
     1621 tcggacgcca tcctgctcag cgacatcctg cgggtcaaca ccgagatcac caaggccccg
     1681 ctgtcggcga gcatgatcaa gcggtacgac gagcaccacc aggacctgac cctgctcaag
     1741 gccctcgtgc gccagcagct gcccgagaag tacaaggaaa tcttcttcga ccagtccaag
     1801 aacggctacg ccggctacat cgacggcggc gcgtcgcagg aggagttcta caagttcatc
     1861 aagccgatcc tggagaagat ggacggcacc gaggagctgc tcgtcaagct gaaccgcgag
     1921 gacctgctcc gcaagcagcg gaccttcgac aacggctcca tcccgcacca gatccacctg
     1981 ggcgagctgc acgccatcct ccggcgccag gaggacttct accccttcct gaaggacaac
     2041 cgcgagaaga tcgagaagat cctgaccttc cgcatcccgt actacgtcgg ccccctggcc
     2101 cgcggcaact cccggttcgc gtggatgacc cggaagtcgg aggagaccat caccccgtgg
     2161 aacttcgagg aggtcgtgga caagggcgcc tccgcccagt cgttcatcga gcgcatgacc
     2221 gccttcgaca agaacctccc gaacgagaag gtcctgccca agcactccct gctctacgag
     2281 tacttcaccg tgtacaacga gctgaccaag gtcaagtacg tgaccgaggg catgcggaag
     2341 ccggccttcc tgtcgggcga gcagaagaag gcgatcgtcg acctgctctt caagaccaac
     2401 cgcaaggtca ccgtgaagca gctgaaggag gactacttca agaagatcga gtgcttcgac
     2461 tccgtcgaga tcagcggcgt ggaggaccgc ttcaacgcct ccctgggcac ctaccacgac
     2521 ctgctcaaga tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctg
     2581 gaggacatcg tcctcaccct gaccctcttc gaggaccgcg agatgatcga ggagcggctc
     2641 aagacctacg cccacctgtt cgacgacaag gtgatgaagc agctgaagcg gcgccggtac
     2701 accggctggg gcgccctctc ccggaagctg atcaacggca tccgggacaa gcagagcggc
     2761 aagaccatcc tggacttcct caagtccgac ggcttcgcca accgcaactt catggcgctc
     2821 atccacgacg acagcctgac cttcaaggag gacatccaga aggcccaggt ctcgggccag
     2881 ggcgacagcc tccacgagca catcgccaac ctggcgggct ccccggcgat caagaagggc
     2941 atcctccaga ccgtcaaggt cgtggacgag ctggtcaagg tgatgggccg ccacaagccc
     3001 gagaacatcg tgatcgagat ggcccgggag aaccagacca cccagaaggg ccagaagaac
     3061 tcgcgcgagc ggatgaagcg gatcgaggag ggcatcaagg agctgggcag ccagatcctg
     3121 aaggagcacc cggtcgagaa cacccagctc cagaacgaga agctgtacct ctactacctc
     3181 cagaacggcc gcgacatgta cgtggaccag gagctggaca tcaaccggct gtccgactac
     3241 gacgtcgacc acatcgtgcc gcagtccttc ctgaaggacg actcgatcga caacaaggtc
     3301 ctgacccgct cggacaagaa ccggggcaag tccgacaacg tgccctcgga ggaggtcgtg
     3361 aagaagatga agaactactg gcgccagctg ctcaacgcca agctcatcac ccagcgcaag
     3421 ttcgacaacc tgaccaaggc cgagcggggc ggcctgagcg agctggacaa ggcgggcttc
     3481 atcaagcgcc agctggtcga gacccgggcg atcaccaagc acgtggccca gatcctggac
     3541 tcccggatga acaccaagta cgacgagaac gacaagctga tccgcgaggt caaggtgatc
     3601 accctcaaga gcaagctggt ctccgacttc cgcaaggact tccagttcta caaggtccgg
     3661 gagatcaaca actaccacca cgcccacgac gcgtacctga acgccgtcgt gggcaccgcg
     3721 ctgatcaaga agtacccgaa gctggagtcc gagttcgtct acggcgacta caaggtctac
     3781 gacgtgcgca agatgatcgc caagagcgag caggagatcg gcaaggccac cgcgaagtac
     3841 ttcttctact ccaacatcat gaacttcttc aagaccgaga tcaccctggc caacggcgag
     3901 atccgcaagc ggcccctgat cgagaccaac ggcgagaccg gcgagatcgt ctgggacaag
     3961 ggccgcgact tcgccaccgt ccggaaggtg ctgtcgatgc cgcaggtcaa catcgtgaag
     4021 aagaccgagg tgcagaccgg cggcttcagc aaggagtcca tcctccccaa gcgcaacagc
     4081 gacaagctga tcgcccggaa gaaggactgg gacccgaaga agtacggcgg cttcgacagc
     4141 cccaccgtcg cctactccgt gctggtcgtg gcgaaggtcg agaagggcaa gagcaagaag
     4201 ctgaagtccg tgaaggagct gctcggcatc accatcatgg agcgctcctc gttcgagaag
     4261 aacccgatcg acttcctgga ggccaagggc tacaaggagg tcaagaagga cctcatcatc
     4321 aagctgccca agtacagcct gttcgagctg gagaacggcc gcaagcggat gctcgcctcc
     4381 gcgggcgagc tgcaaaaggg caacgagctg gccctcccgt cgaagtacgt caacttcctg
     4441 tacctcgcgt cccactacga gaagctgaag ggctcgcccg aggacaacga gcagaagcag
     4501 ctcttcgtgg agcagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     4561 agcaagcgcg tcatcctggc cgacgcgaac ctcgacaagg tgctgtccgc ctacaacaag
     4621 caccgcgaca agccgatccg ggagcaggcg gagaacatca tccacctgtt caccctcacc
     4681 aacctgggtg ccccggccgc cttcaagtac ttcgacacca ccatcgaccg caagcggtac
     4741 acctccacca aggaggtcct cgacgcgacc ctgatccacc agagcatcac cggcctgtac
     4801 gagacccgca tcgacctgag ccagctgggc ggcgactcgg gcggctccac caacctgtcg
     4861 gacatcatcg agaaggagac cggcaagcag ctggtgatcc aggagtccat cctgatgctg
     4921 ccggaggaag tggaggaagt gatcggcaac aagccggagt cggacatcct ggtgcacacc
     4981 gcctacgacg agtcgaccga cgagaacgtc atgctgctga cctcggacgc cccggagtac
     5041 aagccgtggg ccctggtcat ccaggactcc aacggcgaga acaagatcaa gatgctgtga
//
