LOCUS       sequence-42502-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HTATIP (2OU2)
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtacccggat gaagaacatt gagtgcattg
      121 agctgggccg gcaccgcctc aagccgtggt acttctcccc gtacccacag gaactcacca
      181 cattgcctgt cctctacctg tgcgagttct gcctcaagta cggccgtagt ctcaagtgtc
      241 ttcagcgtca tttgaccaag tgtgacctac gacatcctcc aggcaatgag atttaccgca
      301 agggcaccat ctccttcttt gagattgatg gacgtaagaa caagagttat tcccagaacc
      361 tgtgtctttt ggccaagtgt ttccttgacc ataagacact gtactatgac acagaccctt
      421 tcctcttcta cgtcatgaca gagtatgact gtaagggctt ccacatcgtg ggctacttct
      481 ccaaggagaa agaatcaacg gaagactaca atgtggcctg catcctaacc ctgcctccct
      541 accagcgccg gggctacggc aagctgctga tcgagttcag ctatgaactc tccaaagtgg
      601 aagggaaaac agggacccct gagaagcccc tctcagacct tggcctccta tcctatcgaa
      661 gctactggtc ccagaccatc ctggagatcc tgatggggct ga
//
