LOCUS       sequence-425043-        5058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with 
            an sgRNA cloning cassette, facilitating high-efficiency and 
            high-fidelity base editing at targets bearing NG PAM in 
            Streptomyces..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
  TITLE     Engineered cytosine base editor enabling broad-scope and 
            high-fidelity gene editing in Streptomyces.
  JOURNAL   Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
  PUBMED    38971862
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-7"; volume: "15(1):5687. doi"; pages: " 
            10.1038/s41467-024-49987-3"
COMMENT     Sequence Label: peSCBE3-NG-HF1-Hypa
FEATURES             Location/Qualifiers
     source          1..5058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4807..5058
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggaggcct cccccgcctc cggcccccgc cacctgatgg acccgcacat cttcacctcc
       61 aacttcaaca acggcatcgg ccgccacaag acctacctgt gctacgaggt cgagcggctg
      121 gacaacggca cctccgtcaa gatggaccag caccggggct tcctgcacaa ccaggccaag
      181 aacctgctgt gcggcttcta cggccgccac gccgagctgc ggttcctgga cctggtcccc
      241 tccctgcagc tggaccccgc ccagatctac cgcgtcacct ggttcatctc ctggtccccg
      301 tgcttctcct ggggctgcgc cggcgaggtc cgggccttcc tgcaggagaa cacccacgtc
      361 cgcctgcgga tcttcgccgc ccgcatcttc gactacgacc ccctgtacaa ggaagccctg
      421 cagatgctgc gggacgccgg cgcccaggtc tccatcatga cctacgacga gttcaagcac
      481 tgctgggaca ccttcgtgga ccaccagggc tgccccttcc agccctggga cggcctggac
      541 gagcactccc aggccctgtc cggccgcctg cgggccatcc tgcagaacca gggcaactcc
      601 ggctcggaga ccccgggcac ctcggagtcc gccacccccg agtcctccgg ctcggagacc
      661 ccgggcacct cggagtccgc caccccggag tccgacaaga agtacagcat cggcctggcc
      721 atcggcacca acagcgtggg ctgggcggtc atcaccgacg agtacaaggt cccctccaag
      781 aagttcaagg tcctgggcaa caccgaccgg cactcgatca agaagaacct gatcggcgcc
      841 ctgctcttcg acagcggcga gaccgccgag gcgacccgcc tgaagcggac cgcgcgccgg
      901 cgctacaccc ggcgcaagaa ccgcatctgc tacctccagg aaatcttctc caacgagatg
      961 gccaaggtcg acgactcgtt cttccaccgg ctcgaggaga gcttcctggt ggaggaggac
     1021 aagaagcacg agcgccaccc gatcttcggc aacatcgtcg acgaggtggc ctaccacgag
     1081 aagtacccca ccatctacca cctccgcaag aagctggtcg actcgaccga caaggcggac
     1141 ctgcggctca tctacctggc cctcgcgcac atgatcaagt tccgcggcca cttcctcatc
     1201 gagggcgacc tgaacccgga caactccgac gtcgacaagc tcttcatcca gctggtgcag
     1261 acctacaacc agctgttcga ggagaacccc atcaacgcca gcggcgtcga cgccaaggcg
     1321 atcctctccg cgcgcctgag caagtcccgg cgcctggaga acctcatcgc ccagctgccg
     1381 ggcgagaaga agaacggcct cttcggcaac ctgatcgcgc tgtcgctcgg cctgaccccc
     1441 aacttcaaga gcaacttcga cctggccgag gacgcgaagc tccagctgtc caaggacacc
     1501 tacgacgacg acctggacaa cctgctcgcc cagatcggcg accagtacgc ggacctcttc
     1561 ctggccgcga agaacctctc ggacgccatc ctgctcagcg acatcctgcg ggtcaacacc
     1621 gagatcacca aggccccgct gtcggcgagc atgatcaagc ggtacgacga gcaccaccag
     1681 gacctgaccc tgctcaaggc cctcgtgcgc cagcagctgc ccgagaagta caaggaaatc
     1741 ttcttcgacc agtccaagaa cggctacgcc ggctacatcg acggcggcgc gtcgcaggag
     1801 gagttctaca agttcatcaa gccgatcctg gagaagatgg acggcaccga ggagctgctc
     1861 gtcaagctga accgcgagga cctgctccgc aagcagcgga ccttcgacaa cggctccatc
     1921 ccgcaccaga tccacctggg cgagctgcac gccatcctcc ggcgccagga ggacttctac
     1981 cccttcctga aggacaaccg cgagaagatc gagaagatcc tgaccttccg catcccgtac
     2041 tacgtcggcc ccctggcccg cggcaactcc cggttcgcgt ggatgacccg gaagtcggag
     2101 gagaccatca ccccgtggaa cttcgaggag gtcgtggaca agggcgcctc cgcccagtcg
     2161 ttcatcgagc gcatgaccgc cttcgacaag aacctcccga acgagaaggt cctgcccaag
     2221 cactccctgc tctacgagta cttcaccgtg tacaacgagc tgaccaaggt caagtacgtg
     2281 accgagggca tgcggaagcc ggccttcctg tcgggcgagc agaagaaggc gatcgtcgac
     2341 ctgctcttca agaccaaccg caaggtcacc gtgaagcagc tgaaggagga ctacttcaag
     2401 aagatcgagt gcttcgactc cgtcgagatc agcggcgtgg aggaccgctt caacgcctcc
     2461 ctgggcacct accacgacct gctcaagatc atcaaggaca aggacttcct cgacaacgag
     2521 gagaacgagg acatcctgga ggacatcgtc ctcaccctga ccctcttcga ggaccgcgag
     2581 atgatcgagg agcggctcaa gacctacgcc cacctgttcg acgacaaggt gatgaagcag
     2641 ctgaagcggc gccggtacac cggctggggc gccctctccc ggaagctgat caacggcatc
     2701 cgggacaagc agagcggcaa gaccatcctg gacttcctca agtccgacgg cttcgccaac
     2761 cgcgcgttcg ccgcgctcat cgccgacgac agcctgacct tcaaggagga catccagaag
     2821 gcccaggtct cgggccaggg cgacagcctc cacgagcaca tcgccaacct ggcgggctcc
     2881 ccggcgatca agaagggcat cctccagacc gtcaaggtcg tggacgagct ggtcaaggtg
     2941 atgggccgcc acaagcccga gaacatcgtg atcgagatgg cccgggagaa ccagaccacc
     3001 cagaagggcc agaagaactc gcgcgagcgg atgaagcgga tcgaggaggg catcaaggag
     3061 ctgggcagcc agatcctgaa ggagcacccg gtcgagaaca cccagctcca gaacgagaag
     3121 ctgtacctct actacctcca gaacggccgc gacatgtacg tggaccagga gctggacatc
     3181 aaccggctgt ccgactacga cgtcgacgcc atcgtgccgc agtccttcct gaaggacgac
     3241 tcgatcgaca acaaggtcct gacccgctcg gacaagaacc ggggcaagtc cgacaacgtg
     3301 ccctcggagg aggtcgtgaa gaagatgaag aactactggc gccagctgct caacgccaag
     3361 ctcatcaccc agcgcaagtt cgacaacctg accaaggccg agcggggcgg cctgagcgag
     3421 ctggacaagg cgggcttcat caagcgccag ctggtcgaga cccgggcgat caccaagcac
     3481 gtggcccaga tcctggactc ccggatgaac accaagtacg acgagaacga caagctgatc
     3541 cgcgaggtca aggtgatcac cctcaagagc aagctggtct ccgacttccg caaggacttc
     3601 cagttctaca aggtccggga gatcaacaac taccaccacg cccacgacgc gtacctgaac
     3661 gccgtcgtgg gcaccgcgct gatcaagaag tacccgaagc tggagtccga gttcgtctac
     3721 ggcgactaca aggtctacga cgtgcgcaag atgatcgcca agagcgagca ggagatcggc
     3781 aaggccaccg cgaagtactt cttctactcc aacatcatga acttcttcaa gaccgagatc
     3841 accctggcca acggcgagat ccgcaagcgg cccctgatcg agaccaacgg cgagaccggc
     3901 gagatcgtct gggacaaggg ccgcgacttc gccaccgtcc ggaaggtgct gtcgatgccg
     3961 caggtcaaca tcgtgaagaa gaccgaggtg cagaccggcg gcttcagcaa ggagtccatc
     4021 cggcccaagc gcaacagcga caagctgatc gcccggaaga aggactggga cccgaagaag
     4081 tacggcggct tcgtcagccc caccgtcgcc tactccgtgc tggtcgtggc gaaggtcgag
     4141 aagggcaaga gcaagaagct gaagtccgtg aaggagctgc tcggcatcac catcatggag
     4201 cgctcctcgt tcgagaagaa cccgatcgac ttcctggagg ccaagggcta caaggaggtc
     4261 aagaaggacc tcatcatcaa gctgcccaag tacagcctgt tcgagctgga gaacggccgc
     4321 aagcggatgc tcgcctccgc gcggttcctg caaaagggca acgagctggc cctcccgtcg
     4381 aagtacgtca acttcctgta cctcgcgtcc cactacgaga agctgaaggg ctcgcccgag
     4441 gacaacgagc agaagcagct cttcgtggag cagcacaagc actacctgga cgagatcatc
     4501 gagcagatca gcgagttcag caagcgcgtc atcctggccg acgcgaacct cgacaaggtg
     4561 ctgtccgcct acaacaagca ccgcgacaag ccgatccggg agcaggcgga gaacatcatc
     4621 cacctgttca ccctcaccaa cctgggtgcc ccgcgggcct tcaagtactt cgacaccacc
     4681 atcgaccgca aggtctaccg ctccaccaag gaggtcctcg acgcgaccct gatccaccag
     4741 agcatcaccg gcctgtacga gacccgcatc gacctgagcc agctgggcgg cgactcgggc
     4801 ggctccacca acctgtcgga catcatcgag aaggagaccg gcaagcagct ggtgatccag
     4861 gagtccatcc tgatgctgcc ggaggaagtg gaggaagtga tcggcaacaa gccggagtcg
     4921 gacatcctgg tgcacaccgc ctacgacgag tcgaccgacg agaacgtcat gctgctgacc
     4981 tcggacgccc cggagtacaa gccgtgggcc ctggtcatcc aggactccaa cggcgagaac
     5041 aagatcaaga tgctgtga
//
