LOCUS       sequence-425045-         149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET31b plasmid with short insert lacking ribosome binding site to 
            increase 2',3'-cNMP levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 149)
  AUTHORS   Chauhan SS, Marotta NJ, Karls AC, Weinert EE
  TITLE     Binding of 2',3'-Cyclic Nucleotide Monophosphates to Bacterial 
            Ribosomes Inhibits Translation.
  JOURNAL   ACS Cent Sci. 2022 Nov 23;8(11):1518-1526. doi: 
            10.1021/acscentsci.2c00681. Epub 2022 Sep 21.
  PUBMED    36439312
REFERENCE   2  (bases 1 to 149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.2c00681"; journalName: "ACS Cent Sci"; date: 
            "2022-11-23- 23"; volume: "8"; issue: "11"; pages: "1518-1526"
COMMENT     Sequence Label: pNoRBS
FEATURES             Location/Qualifiers
     source          1..149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      96..143
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agatcttaat acgactcact ataggactag tcgtcggcga aggacgggtc cagcgttcgc
       61 gctgttgagt agagtgtgag cgccctcgta cagccctagc ataacccctt ggggcctcta
      121 aacgggtctt gaggggtttt ttgctcgag
//
