LOCUS       sequence-425046-        8635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acetyl-CoA biosensor in the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Smith JJ, Valentino TR, Ablicki AH, Banerjee R, Colligan AR, Eckert 
            DM, Desjardins GA, Diehl KL
  TITLE     A genetically encoded fluorescent biosensor for visualization of 
            acetyl-CoA in live cells.
  JOURNAL   Cell Chem Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002.
  PUBMED    39874963
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002."
COMMENT     Sequence Label: Nuclear PancACe
FEATURES             Location/Qualifiers
     source          1..8635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             571..594
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             607..633
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    649..673
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1107..1128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1234..1255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1495..1514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1877..1994
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2043..2553
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2472..2490
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2575..3174
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2575..2594)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3071..3091
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3302..3535
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3607..3728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3644..3663)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3698..3717
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3768..3903
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3923..3942)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3924..3942)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3952..3969)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3952..3968)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3961..3983)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4110..4565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4197..4216)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4407..4428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4591..4695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4696..4764,4765..5556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4914..4933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5727..6315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6216..6235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6469..6486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6603..6624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6639..6644,6645..6662,6663..6669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6677..6693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6682..6704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6701..6717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6701..6717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6736..6756
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6738..6756
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6784..7010
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7011..7191
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7238..7363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7856..8089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8274..8318
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(8616..8635,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
      541 gtgaccggtc caaagaagaa gcgtaaggta gactacaagg atgacgatga caagctcgat
      601 ggaggatacc catacgatgt tccagattac gctgctcgag gttatcaaac aagtttgtac
      661 aaaaaagcag gctccaccat ggggcatatg aagctgacaa tcattcgcct ggagaaattt
      721 tccgaccagg atagaatcga ccttcagaag atctggcccg aatattctcc cagtagcctc
      781 caagtagacg acaaccatcg aatctacgcc gcgagattta atgaacgact gcttgcggcg
      841 gttagggtaa ccctcagcgg tacggaaggt gctctggact ccctgcgggt ccgaggagca
      901 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc
      961 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
     1201 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1261 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     1321 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1381 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1441 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     1501 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1561 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1621 aacggtgctg aggtgacaag acggagaggg gtaggacaat atttgctcga ggaggtcctt
     1681 agaaataacc ccggagtaag ttgctggtgg atggcggacg ctggagttga ggatcgcgga
     1741 gtcatgacgg ctttcatgca ggcccttggg ttcaccgcac agcaaggggg ctgggagaag
     1801 tgctaatccg gactcagatc tcgagctcaa gcttcgaatt ctcgacctcg agacaaatgg
     1861 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1921 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1981 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     2041 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     2101 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     2161 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     2221 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     2281 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     2341 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     2401 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     2461 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     2521 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc
     2581 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     2641 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     2701 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     2761 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     2821 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2881 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2941 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3001 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3061 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3121 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     3181 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta
     3241 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3301 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3361 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3421 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3481 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     3541 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3601 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3721 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3781 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3841 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3901 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3961 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4021 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4081 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4141 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4201 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4261 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4321 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4381 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4441 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4501 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4561 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4621 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4681 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4741 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4801 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4861 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4921 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4981 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5041 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5101 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5161 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5221 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5281 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5341 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5401 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5461 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5521 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5581 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5641 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6361 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6421 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6481 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6541 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6601 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6661 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6721 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6781 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6841 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6901 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     6961 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7201 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7261 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7321 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7381 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7441 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7501 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7561 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7621 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7681 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8221 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8281 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8341 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8401 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8461 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     8521 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat
     8581 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgat
//
