LOCUS       sequence-425048-        8254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cpGFP in the cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Smith JJ, Valentino TR, Ablicki AH, Banerjee R, Colligan AR, Eckert 
            DM, Desjardins GA, Diehl KL
  TITLE     A genetically encoded fluorescent biosensor for visualization of 
            acetyl-CoA in live cells.
  JOURNAL   Cell Chem Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002.
  PUBMED    39874963
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002."
COMMENT     Sequence Label: Cytoplasmic cpGFP
FEATURES             Location/Qualifiers
     source          1..8254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             583..606
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             619..645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    661..685
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     906..927
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1033..1054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1294..1313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1496..1613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1662..2172
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2091..2109
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2194..2793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2194..2213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2690..2710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2921..3154
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3226..3347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3263..3282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3317..3336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3387..3522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3542..3561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3543..3561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3571..3588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3571..3587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3580..3602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3729..4184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3816..3835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4026..4047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4210..4314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4315..4383,4384..5175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4533..4552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5346..5934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5835..5854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6088..6105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6222..6243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6258..6263,6264..6281,6282..6288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6296..6312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6301..6323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6320..6336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6320..6336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6355..6375
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6357..6375
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6403..6629
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6630..6810
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6857..6982
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7475..7708
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7893..7937
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(8235..8254,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
      541 gtgaccggtc tgcagaaaaa gctggaagag ctggaactgg acgactacaa ggatgacgat
      601 gacaagctcg atggaggata cccatacgat gttccagatt acgctgctcg aggttatcaa
      661 acaagtttgt acaaaaaagc aggctccacc atggggcata acgtctatat caaggccgac
      721 aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcggc
      781 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      841 cccgacaacc actacctgag cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc
      901 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      961 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
     1021 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1081 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1141 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1201 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1261 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1321 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1381 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actaatccgg actcagatct
     1441 cgagctcaag cttcgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     1501 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     1561 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     1621 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
     1681 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     1741 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     1801 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     1861 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     1921 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     1981 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     2041 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     2101 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     2161 ctctctcccc agggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc
     2221 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     2281 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     2341 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     2401 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     2461 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     2521 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     2581 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     2641 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     2701 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     2761 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     2821 accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac ttacaaggca
     2881 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2941 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     3001 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3061 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3121 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     3181 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     3241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     3361 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     3541 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     3601 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3661 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3721 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3781 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3841 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3901 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3961 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4021 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4081 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4141 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6241 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6301 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     6361 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     6421 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     6481 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     6541 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     6601 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6661 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6721 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6781 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6841 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6901 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6961 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7021 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7081 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7141 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7201 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7261 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7321 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7381 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7441 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7501 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7561 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7621 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7681 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7741 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7801 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7861 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7921 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7981 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8041 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8101 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     8161 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg
     8221 ccgccccctt caccgagggc ctatttccca tgat
//
