LOCUS       sequence-425049-       16658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BGC08 pathway. Native promoter and CDS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
  TITLE     Cross-kingdom expression of synthetic genetic elements promotes 
            discovery of metabolites in the human microbiome.
  JOURNAL   Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 
            10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
  PUBMED    35366417
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.008"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1487-1505.e14"
COMMENT     Sequence Label: pPath-BGC08-native
FEATURES             Location/Qualifiers
     source          1..16658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..36
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        292..635
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             666..1475
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDVSD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(733..752)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1481..1678
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             1815..1832
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1885..1903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1899..1946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        11367..11458
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11459..11527,11528..12319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      12323..12362
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            12509..12617
                     /note="incP origin of transfer"
     rep_origin      12636..13024
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             14822..16639
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     promoter        complement(16640..16658)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(16641..16658)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gggtgccagg gcgtgcccgt gggctccccg ggcgcgtata gggataacag ggtaatacgt
       61 caaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
      121 ctttgaaaac aactttgaaa aggctgtttt ctgtatttaa ggttttagaa tgcaaggaac
      181 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
      241 aaaagaaatt gcataccttt gttcctcggt tatatgtttg ctcatctgca agacatggag
      301 gcccagaata ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt
      361 cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat acattttgat
      421 ggccgcacgg cgcgaagcaa aaattacggc acctcgctgc agacctgcga gcagggaaac
      481 gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa
      541 ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga
      601 tacagttctc acatcacatc cgaacataaa caaccaaaaa tttgtaatta agaaggagtg
      661 attacatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg
      721 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      781 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      841 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      901 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      961 tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1021 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1081 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1141 ttgatgtgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1201 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1261 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1321 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1381 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1441 tgcagtttca tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct
     1501 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     1561 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgtccag atgcgaagtt
     1621 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     1681 ctagcataac cccgcggggc ctctttcggg gatctcgcgg ggttttttgc tgaaagaagc
     1741 ttcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttatgtt gttgtcgctg
     1801 cggccgcact cgagcaccac caccaccacc actgggatcc ggctgctaac aaagcccgaa
     1861 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     1921 ctaaacgggt cttgaggggt tttttgctga aggccatcat ttcatgtcat tccgagaaag
     1981 aagaatgatg gcattaatgc atggccaaat tttgaaacga aacctaataa taccagtgat
     2041 ttttttgaaa aagtaaaatt ttagtattat cagcaagata atatttttaa tgaattgttc
     2101 taaaattaat atgccatacg tgtaaaatta aggcattcat tatgacagcc attttagaat
     2161 caaaacagta taaaaaacca tactccgata gaatatcgaa atatggtttt aaacaatata
     2221 ttttacaaga aaattttatt agaatgagaa gcacataaat tgactattgg agaagcacta
     2281 aaattcatac agcataatag aatgcttact gaggaagaaa tgtcaaaagg tataattgga
     2341 aaaggtacat attctaaagt aattcatggc aaacaaaatt taagttcatg tcttttaata
     2401 aaaatattat tagaaaatga cattgatatt gattatttta tatctttaat aaaaaaagat
     2461 tattcttcaa aagagtcttt gaaagagaat atgctctctc aaagaatggc tgtagcattt
     2521 aataatcata aaattaatga cgcagaagaa tgtttgagag aaatcaagaa gttaggtacg
     2581 aatcccctat ttgagcaacg tgctataatt gcagttcatt acttaaaaga tcagctagat
     2641 agtcttagtt tagaatttaa aaaaacaatt attgaagagt ttggtaagac agataattgg
     2701 atattgaatt tagatatgtt aagactattt ggttctgcta tgatgattct tccaccaaaa
     2761 gaaatagaat tggagatgca attattcttc tttagattaa atagaatgaa aatagaatcc
     2821 ccgaatatga tagaaagata cgctattttg tgttgtaatt atttacattg gaaatatacc
     2881 tataatagac atttagataa taatacaacg aagtgcatta attttttaaa attcttaccc
     2941 aaaagctcac gtatgttttt atacactgta tcgggtaagt attattatta cttatttaat
     3001 aatcaggtag aagaagcaaa aaatataaaa gaaagcttac taaagctggg ttgtactgaa
     3061 ggcgtagaaa attggccaat ttaatagtaa aaatataata tttgtatttt atttgaagca
     3121 taaaaatttg tagagtctct taagaaaagt gaggttatat caccaataaa atgtgatata
     3181 atctcacttt tttgttttat ttttgttagc ctttttcatt cctctatcca aaggtatttt
     3241 attataatat ttaatttttt atttaatagg tgctaagtaa aagcggtttg aaatacaagc
     3301 cctaaataaa ctagagacat cgttcagtat aactaactac caatatattt aactgtttaa
     3361 aattttcgat actcttaaca taaaaaaata gtttctgata ggaaacctaa atcaatattg
     3421 aaaataaata gtgatattat ggcaaataat aagtaattga aaaggggtga aaattatgga
     3481 ttggtggctt ttcccggtta ttttaaaata gaaaattaaa aatttaggaa gtttaaaaat
     3541 gaaaaaagaa agaaaaaata ttttcctttt gcttagtgct aaggtgattt ctaatattgg
     3601 agataatttt tttgatttta gtaataatac ttggatagca tctctaggtg caaaaggtca
     3661 gaaaatactt actatatatc aaataacgga atcaataacc ggtattgtat taaatgtgtt
     3721 tggtggagtg tttgctgata actataatag aaaaaaaatt attaccataa ctgacttaat
     3781 agctggtgta gcatgtttag tcttaagtat agtatatcct accaaatttt ttgtttttgc
     3841 aatgatattg gtaaatataa tactagctat actttcatct tttagatcac cggcatataa
     3901 atcaattatt ccaaatacag ttcacaaaga taaaatagta aaagtaaatt ctaatttaga
     3961 aatttgtaat caacttgtta gtgttagttc gccagtaatt tcattgattg cttataaagt
     4021 ttttgggata tgtggagcac ttttgataga tggaatatct tttttaatat cagcacttct
     4081 aaataactgt ttacaagtaa tccataaaaa aagtgacgaa aatattaggc atataaaaaa
     4141 taaaattatt aagaacatta ttgaaggtat tgaatacatt actaaaaatt cacaagtagc
     4201 aatattactt attgtctcat cagctgttaa ttttttctta gctggatata attactcttt
     4261 tccttttgtt aataaagctt ttggaacagg aaatagtaca tatactattt tattagtaag
     4321 tcaatcttta ggtggaatta tcggaggaat tattagtccc aaattgacta aaaacgattc
     4381 tattagtcat ttgcttaatc tattaacatt ttgtggatta agcatcgcag gaatctattt
     4441 attgtttcaa tggtatcatt ccttattaac agtttttgtt tttatgtttt tatttcatat
     4501 gttgctaaca gcatacaata ttgatttttt tagcataatc caaacaaaag tagatgataa
     4561 gtatctaggg agagtgttta gtgtagtctt tactgttgcc attattttta tgcctatagg
     4621 gacagccgta ttttcgtatt attcaaaagc ttatgtggta gaaaattaca taataattgg
     4681 tattgggata ataatcgttt caattgtagc tcaagttttg agcagattta agggaagtaa
     4741 ataaatgaag ttcgatgaag aatattacaa agaagtgata aaaaagtaca attataatcc
     4801 tttattgttt aataagattg attttcaatt gccaggtatt tctaaaaatg aaatgcaatt
     4861 aatgtggctg tgtagttacc attctttaaa aaataggaaa gttaactgtg aaaatactct
     4921 atttacaaca ggaataggtc tctcaggtgt tccacatatg ggtacaatat cacaaataat
     4981 tcgtgctatt aagttacaac gaggaggcaa taaagttcaa atagtgttgg gagatataga
     5041 tgcgtataca ggcagacata tttcatggac acaaacacag catttaaagg aaaaatatag
     5101 aaattttata ttaaaattag gtttcaattc tcaaacaggt atactaagag ctcaaagtga
     5161 taaaagtgta tttttgactc aagcaaagat tgcagggtat tttaaagatg aaattgctga
     5221 tgaatctgaa gaagatttgc acgacttata tgttaaagat aacaaagttg aatctaaaat
     5281 gacgtttaat agaaaatatt cgttatcttt aatgtgtgca gattttattg aaccattatt
     5341 aatggataat tttacgaata tagtagtttt tttaggactt gatgaacata aatacgttag
     5401 agcgtcaaaa caagttatgg atttaatgaa acaagaattt aaagttttag attccaaaaa
     5461 aatatatgct ttatattcgc ctttaattag aggttttaat ggctttccta agatgtcgaa
     5521 aagctttaca gaatcctctt taagtattca aaatacgcct tttgaaatag aacataaaat
     5581 tatgaataaa aaagaagatg agatgcctct tcaactaaat gtaaactatc aattaatgaa
     5641 aggtttaatg gatatatccg aagaaaaagc aaagcaggtt cattcagatt ataaaaacca
     5701 aaatgtagga tggattgtag ataaagaaga attattaaaa agagtaatag aaattagaga
     5761 tatgtggtat gaatgataaa tttaaccaag ttaacttaat tagtaaacag ttacagcaaa
     5821 ttgtaaatga caaaaaatat gacattttag aactaaagga tattctttat tatttaatgt
     5881 caagtgataa aaaatattca gatgcattct taagttcttt attaatggga ttagaacttg
     5941 taaacatgcc gagtaaagac ataaaaactt tgattgatgt aataattaga attaatcatt
     6001 ataatccttt ctcagtttcc ttatctaaat atcctaatat tacttgcgta gcgggttctg
     6061 gaaaaaaata ttataaaact ttgaatatta caactttagc tacaattgta gcttcgggat
     6121 taggagcagt aatcgttaaa ccaacttcca agggtgttac ttcacgttta ggttcagaag
     6181 atttgcttga atatttaaaa cttccaataa ctaattctat aactaaagct gaggcattta
     6241 tttctaaaaa ttctattgca tttttttcta tagaaaattt gattcccaag tttgatgcaa
     6301 tctatggagg acgacagtta acaatatctc cattaagtaa tgctttgccg gggttaatgt
     6361 cgcccatatc atgccaaaat atatattttg gattaagttc taattatcat aacaaggctc
     6421 ttaatttatt gcgatcatat ggaattggag gcaatatatc aatatctagt acaaaattaa
     6481 acgaaggata tttagatgaa ttaacattta gcagtggttc taagctgact tttgaatctt
     6541 ctaataattc aacaattatt ttagaaggta ttacttttga caaagctgat ttaaataatg
     6601 caaggtcaac aaataatata actaataata taaataaaat tatttcagaa ataaaaagta
     6661 cagaagttac ggactatatg aaaatagtat gcttgaacgc tgctggtatt ttgcttactg
     6721 caggagtgta taaaaatatc agagtagcgt atgaacaagc aaagttatat gtacaaaagg
     6781 gtaaattatt agatagaata aatgcattaa ggggtaaaca attatgagtc tatttgaaca
     6841 gttattgaat ttaaagcaga gtccaaaagt agttgttaag gattgtaata atgattactc
     6901 tgggaggcaa ttattagaaa aagtaggtaa aattcaaaat gtactactac acctgggggt
     6961 taaaaatgga gataaaattc ctgtaatagt ggagccttct atagatgcaa ttgctttagt
     7021 gtatgctatt ggattcttgg gtgccgtata tgttcccatc tcccctaaat cgcccaaaaa
     7081 acgtattaat tttattataa aagatttgag tgctaaattg gtacttacaa atcagaatta
     7141 tctattgata gataataata taaatatgtt tacaataaaa gatctactaa gtatgacaat
     7201 caaggataaa acgcctacag ttatgtctag acgtcaaaat gatactgctt atattattta
     7261 tacatctgga agcacaggta accctaaagg ggtagaagta tcttttgata atttagagtc
     7321 atttattcat tctttcaaca tttgtttccc aagttcccaa aattcaactt acttattaaa
     7381 tacagcttta caatttgacg tatcaatttc tgaattatat ggatggattt ctcaaggagc
     7441 ggttcttcaa ttactttcag cagaacaatt aaaagattta aaaaacttac cactttatgt
     7501 aaagaaatat catattactc atttatgtgc ggcaccatct acattacaag tcatggataa
     7561 tagccgacta gatcttcttg acgagagtag tttatcattc ctattaattg caggtgaaga
     7621 atttcctgtt tctttggcaa agagacttaa aaagcttgta aaaagtggga aagtatataa
     7681 ttgttatggt ccaacagaag caacagttta tgcgttatat cataaagtta ctatgaaaga
     7741 catattacaa ggaaagatac ctatcggacg cccatttcct aacgctacta ttaagttgtt
     7801 acctcagaaa agaaaaaatt ttgaattact tattggtgga tatggagtgt ccaaaggcta
     7861 tttgaattta aaaagaaaaa atagctacag ctttgttgag ctagatggag aaagatttta
     7921 taaaactgga gatcttgtaa aatatgacca agatatgaat aatattattt ttgttggaag
     7981 aaaagatttt caattagaaa taaatagtat tcgggtagag ccaagtgaaa ttgaaggtgt
     8041 aattaatcaa gttgataaag tgattgacag tttagttgtc atgaataagg ggatattaac
     8101 gtgtatttac acaacgttta agcattctat agttgattct aacagtatta aactgaaaat
     8161 ttcagaaaaa ctaccgaaat atatgattcc taagcggtgg tacagagttt cagagtttcc
     8221 tttaactata aatggaaaaa ttgatagaaa aacgttgtta aagacctttt ctaatcccat
     8281 taataattct gatactgaag ataaattaat aagtaaaatt gaacaaattt gtgatatttc
     8341 taatttatcc gaaaatgaca atatttttga tcttggtatt gattcgttga tgaccgttga
     8401 aatagaaatt atgctagaaa aacattacca ttcgaaaata ccaacaggtt ttttatatga
     8461 acatccaaca gttgaaaaaa taaaacgtta ttttaaagat gaatctaagc ttaaattcaa
     8521 taatattgaa caaattttga aaaagataac agaacgttac gtaattgata aagataaatt
     8581 gattatatat tcaaatgata aaaatgttga aaacaagttg caaaagcatc tttataactt
     8641 atctttattc aaagaaatta aaatcaatga tatttcaatt tttaacaacg aaaacaattg
     8701 tgaaacggtt gttgatggtg gaaaaataaa cgaagatagg aactatgaat cttcaatatt
     8761 tcaaaaagta tatactacta ttaaacttcc aagttttact gtagttagat tttctatcca
     8821 agatgatcta gtagggagta aaacaaaaac attaattact cgattaatat atacaacaga
     8881 agcttttcgt acgaaaatta ttaaaactac tgatggaaat tatattagta cggtttatac
     8941 agatcttaat ataagaagta acatcgaaga tataacatat ttgtctaaag aaaagcaagt
     9001 taattatata aataagaaaa ttggaaaata tgaaaaccaa attttatcta aattaacaag
     9061 aaactatttg tataaaattt tgtcttttag aactagttcc caaaatattg aattagtaat
     9121 tatttgtcat cataatattt gtgatggagc aagcgaacaa ttgctaaaca agaaaattgg
     9181 gcaactctta aataatcaat cagtgactga tttgaagatg aaagattatt tagaaacaat
     9241 aaaaatgaat tctgccattg aaaacatact taatgatcct gtaattaaag aaataggtaa
     9301 aatgaaaaac cagataggta atgagaagct gacttggagt gaaaaaagat tagtaggagt
     9361 tgaaaaatta aataattttc aaaaaatgat gatggtagcc aattatgtta caaaagaata
     9421 tttaaaagaa agcgggcatc aatttatgac atttcaaatg ctgtttaatt ttagaaaggt
     9481 aaataacaaa gatttttcaa ctttgattaa tgattgtcat gaaacattaa ctttctttag
     9541 aaggaagtca gatacagata ttcagtttgt taataatttg ttttatagat taaatgattt
     9601 tcactataat cgtggaataa gtaccggtta tgccatttat aataattttc ctgcatttaa
     9661 taatgaacag cttcaaatac aaaaaatttt ggaagaagct ccaattaata ttgactatat
     9721 aggtgaagtg gaaacaaaga acctagatag taaagcaaaa gcgattatta atttgcgaaa
     9781 gcaattaaaa gatcttaaac atcaaattag gtttactgca ttttctagca aaaatgagat
     9841 gtttatttac cgtatctcag gagaacagac atggcaatat tgaaaattaa tgtaagtgat
     9901 actatccctc tatctcaaga tataactaag agattaataa atgttaatta tgtaggtatt
     9961 tgtggatcag ataagcaaaa attagatgat ccacctatta aatatttagg acatgaaata
    10021 attggaaagg atatggataa cggaaaattt gttgtaataa atccaaatat tacatgtggt
    10081 aaatgttata gttgcctaaa tggttatgaa aatttatgta gcaaagaaaa ggctataggg
    10141 actaatgtga atggtggttt tcaaggttgg ttacgagttc catctactaa tctagaatat
    10201 atagaaaaac cagatattaa gtatatattt acagatcctt tagcagttgt tctgcatggt
    10261 atgtcaatga taaaaatttt atctaattat aaagttattg tattaggcaa tggtagtata
    10321 gcaaaaatat tagtatggcg attaaatttg ttaggtatca agccagatgt tatgtgcaga
    10381 tctggaattt ggaataataa ggaattaacc atatcgaatt tatataaatt gacggaaaaa
    10441 gtatcgctta attactacga tatagctttt gagtgtatag gttttaaaca gtcggaaact
    10501 atagaaaaat cattaggaat gattagacca aaaggagaat ttatttgttt cggtgttttt
    10561 ccagaggact attctccaac attaaaagtt agaaggttat ttgaaaaaga aataactata
    10621 ttgggagttc gatcatttac gaaaaaagat tttaaatacg ccaaaaacat attagatctt
    10681 catggtagta aattaataag tagattatgt ataagcgttg tatctagtga aaacataaat
    10741 gctattatga atgatataga agatagaaat attgataaag taattgttag aatgaagtga
    10801 tagttttaaa gattgcgttt tttgtaatat tatctctaag aatcttaaaa gtaatattat
    10861 tcatgaaaat aaaggtgcca ttgttataat gtcggataat cctgaagtag agggacatat
    10921 gcttgttttt gcaaaaaagc attttcctac attggaactg gcagatcaga gttcttggta
    10981 ctatattggt gaaatattaa atgaattagg aaagagacta aggtcccaag gatatgatgg
    11041 atataattta ttgagtgcta acggtgtatc tgcaggtcaa tccgttaaac attttcactt
    11101 tcatgtcatt ccgagaaaga agaatgatgg cattaatgca tggccaaatt ttgaaacgaa
    11161 acctaataat accagtgatt tttttgaaaa agtaaaattt taggtgaggc cggaccaaaa
    11221 cgaaaaaagg cccccctttc gggaggcctc ttttctggaa tttggtaccg aggggtgcca
    11281 gggcgtgccc cagggctccc cgggcgcgta taatcgattt aaattagtag cccgcctaat
    11341 gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta
    11401 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    11461 gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt
    11521 gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg
    11581 cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga
    11641 agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg
    11701 tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt
    11761 ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg
    11821 cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg
    11881 cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga
    11941 tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc
    12001 ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag
    12061 ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag
    12121 cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg
    12181 tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac
    12241 caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag
    12301 cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
    12361 gacgaccctt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
    12421 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
    12481 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
    12541 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
    12601 ctctgcgagg ctggccgtag gccggccggc gcgccgatct gaagatcagc agttcaacct
    12661 gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttaacgtact
    12721 aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc tcatggctaa
    12781 cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat
    12841 aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat
    12901 aaggctttta aagcctttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca
    12961 ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct
    13021 gacatggggc gcgcccaggt cagtcacagt ggagcctagc actcactcag cgtgacggct
    13081 cagagcagaa ttcacgagcc agaaatagta acttttgcct aaatcacaaa ttgcaaaatt
    13141 taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt aaaatacttt
    13201 tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg attaccgaaa
    13261 cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc actacctagg
    13321 agcggccaaa atgccggctt acattttatg ttagctggtg gactgacgcc agaaaatgtt
    13381 ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca
    13441 aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaataggtt
    13501 attactgagt agtatttatt taagtattgt ttgtgcactt gcctgcaagc cttttgaaaa
    13561 gcaagcataa aagatctaaa cataaaatct gtaaaataac aagatgtaaa gataatgcta
    13621 aatcatttgg ctttttgatt gattgtacag gaaaatatac atcgcagggg gttgactttt
    13681 accatttcac cgcaatggaa ttaaacttgt tgaagagaat gttcacaggc gcatacgcta
    13741 caatgacccg attcttgcta gccgaattcc agtcaggctg ctagcaccag agctacgtga
    13801 ccgcaggact agctccagct gagcgacagc ggcaagaaag ccatccacgc cgaaaacccc
    13861 gcttcggcgg ggttttgccg catcttgccg taactgacaa attaaccgaa ggtttcttcc
    13921 ggccactggg atgcaatgac cttaccaaca acgctacagg attcgttgca cgggatcatt
    13981 gggtattgcg gattcagcgg ctgcaggaaa acttggccag agtcgcggat cagcttctta
    14041 aaggtgaatt catcaccacc caggcgcgca atgcaaaagt ctcccggctc aaccgcctgc
    14101 tcaggatcaa ccaggatcag cataccatcc ggaaatgacg gcttgctgcc cgtcggggcc
    14161 gtcatgctgt tgccttctac ttccagccaa aacgcgctat cgctcgcttt tttggtagtc
    14221 gatacccaac gctcagcgtc acctttggtg aaggtacgca gttccgggct aaacatacct
    14281 gcttgaacgt ggctgaagac cgggtattcg tattcagagc gcagagacgg ttgcatgcta
    14341 accgcttcat acatttcata gatttcacga gctatagaag gagagaactc ctcgacggaa
    14401 actttcagaa tcttggtcag cagtgcggcg ttgtacgcgt tcagcgcatt aataccgtta
    14461 aacagagcgc caacgccgct ctgacccatg cccatcttgt cggctacgct ttcctgagac
    14521 agacccagct cattcttttt cttctcatag attgctttca gacgacgtgc gtcctctagc
    14581 tgctcttgtg taagcggctt tttcttcgtt gacattttga tcctccttta tatggaggta
    14641 ctaaatcgga acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg
    14701 actattttac ctctggcggt gataatggtt gcatcaaatt tgcgcgccac attattattc
    14761 atacctttgt ggaccgtatt acaaagagaa gtgttaaatc aaacaaaaag gaggattaat
    14821 catggacacg tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgaaa attcgagcgc
    14881 agcaagccca gcgacacagc gtagcgccaa cgaagacaag gcggccgacc ttcagcgcga
    14941 agtcgagcgc gacgggggcc ggttcaggtt cgtcgggcat ttcagcgaag cgccgggcac
    15001 gtcggcgttc gggacggcgg agcgcccgga gttcgaacgc atcctgaacg aatgccgcgc
    15061 cgggcggctc aacatgatca ttgtctatga cgtgtcgcgc ttctcgcgcc tgaaggtcat
    15121 ggacgcgatt ccgattgtct cggaattgct cgccctgggc gtgacgattg tttccactca
    15181 ggaaggcgtc ttccggcagg gaaacgtcat ggacctgatt cacctgatta tgcggctcga
    15241 cgcgtcgcac aaagaatctt cgctgaagtc ggcgaagatt ctcgacacga agaaccttca
    15301 gcgcgaattg ggcgggtacg tcggcgggaa ggcgccttac ggcttcgagc ttgtttcgga
    15361 gacgaaggag atcacgcgca acggccgaat ggtcaatgtc gtcatcaaca agcttgcgca
    15421 ctcgaccact ccccttaccg gacccttcga gttcgagccc gacgtaatcc ggtggtggtg
    15481 gcgtgagatc aagacgcaca aacaccttcc cttcaagccg ggcagtcaag ccgccattca
    15541 cccgggcagc atcacggggc tttgtaagcg catggacgct gacgccgtgc cgacccgggg
    15601 cgagacgatt gggaagaaga ccgcttcaag cgcctgggac ccggcaaccg ttatgcgaat
    15661 ccttcgggac ccgcgtattg cgggcttcgc cgctgaggtg atctacaaga agaagccgga
    15721 cggcacgccg accacgaaga ttgagggtta ccgcattcag cgcgacccga tcacgctccg
    15781 gccggtcgag cttgattgcg gaccgatcat cgagcccgct gagtggtatg agcttcaggc
    15841 gtggttggac ggcagggggc gcggcaaggg gctttcccgg gggcaagcca ttctgtccgc
    15901 catggacaag ctgtactgcg agtgtggcgc cgtcatgact tcgaagcgcg gggaagaatc
    15961 gatcaaggac tcttaccgct gccgtcgccg gaaggtggtc gacccgtccg cacctgggca
    16021 gcacgaaggc acgtgcaacg tcagcatggc ggcactcgac aagttcgttg cggaacgcat
    16081 cttcaacaag atcaggcacg ccgaaggcga cgaagagacg ttggcgcttc tgtgggaagc
    16141 cgcccgacgc ttcggcaagc tcactgaggc gcctgagaag agcggcgaac gggcgaacct
    16201 tgttgcggag cgcgccgacg ccctgaacgc ccttgaagag ctgtacgaag accgcgcggc
    16261 aggcgcgtac gacggacccg ttggcaggaa gcacttccgg aagcaacagg cagcgctgac
    16321 gctccggcag caaggggcgg aagagcggct tgccgaactt gaagccgccg aagccccgaa
    16381 gcttcccctt gaccaatggt tccccgaaga cgccgacgct gacccgaccg gccctaagtc
    16441 gtggtggggg cgcgcgtcag tagacgacaa gcgcgtgttc gtcgggctct tcgtagacaa
    16501 gatcgttgtc acgaagtcga ctacgggcag ggggcaggga acgcccatcg agaagcgcgc
    16561 ttcgatcacg tgggcgaagc cgccgaccga cgacgacgaa gacgacgccc aggacggcac
    16621 ggaagacgta gcggcgtaat ctatagtgtc acctaaat
//
