LOCUS       sequence-425050-        8647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acetyl-CoA biosensor in the cytoplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Smith JJ, Valentino TR, Ablicki AH, Banerjee R, Colligan AR, Eckert 
            DM, Desjardins GA, Diehl KL
  TITLE     A genetically encoded fluorescent biosensor for visualization of 
            acetyl-CoA in live cells.
  JOURNAL   Cell Chem Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002.
  PUBMED    39874963
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002."
COMMENT     Sequence Label: Cytoplasmic PancACe
FEATURES             Location/Qualifiers
     source          1..8647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             583..606
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             619..645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    661..685
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1119..1140
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1246..1267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1507..1526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1889..2006
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2055..2565
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2484..2502
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2587..3186
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2587..2606)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3083..3103
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3314..3547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3619..3740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3656..3675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3710..3729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3780..3915
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3935..3954)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3936..3954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3964..3981)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3964..3980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3973..3995)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4122..4577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4209..4228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4419..4440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4603..4707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4708..4776,4777..5568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4926..4945)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5739..6327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6228..6247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6481..6498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6615..6636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6651..6656,6657..6674,6675..6681)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6689..6705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6694..6716
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6713..6729
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6713..6729
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6748..6768
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6750..6768
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6796..7022
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7023..7203
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7250..7375
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7868..8101
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8286..8330
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(8628..8647,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
      541 gtgaccggtc tgcagaaaaa gctggaagag ctggaactgg acgactacaa ggatgacgat
      601 gacaagctcg atggaggata cccatacgat gttccagatt acgctgctcg aggttatcaa
      661 acaagtttgt acaaaaaagc aggctccacc atggggcata tgaagctgac aatcattcgc
      721 ctggagaaat tttccgacca ggatagaatc gaccttcaga agatctggcc cgaatattct
      781 cccagtagcc tccaagtaga cgacaaccat cgaatctacg ccgcgagatt taatgaacga
      841 ctgcttgcgg cggttagggt aaccctcagc ggtacggaag gtgctctgga ctccctgcgg
      901 gtccgaggag caaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
      961 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1021 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     1081 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1141 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     1201 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1261 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     1321 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1381 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1441 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     1501 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1561 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1621 aagctggagt acaacggtgc tgaggtgaca agacggagag gggtaggaca atatttgctc
     1681 gaggaggtcc ttagaaataa ccccggagta agttgctggt ggatggcgga cgctggagtt
     1741 gaggatcgcg gagtcatgac ggctttcatg caggcccttg ggttcaccgc acagcaaggg
     1801 ggctgggaga agtgctaatc cggactcaga tctcgagctc aagcttcgaa ttctcgacct
     1861 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     1921 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     1981 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     2041 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     2101 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     2161 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     2221 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     2281 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     2341 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     2401 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     2461 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     2521 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag
     2581 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2641 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2701 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2761 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2821 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2881 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2941 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     3001 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     3061 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     3121 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     3181 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     3241 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3301 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3361 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     3421 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     3481 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     3541 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3601 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3721 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3781 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3841 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3901 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3961 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4021 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4141 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4201 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4261 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4321 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4381 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4441 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4501 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4561 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4861 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4921 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5581 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5641 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6541 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6601 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6661 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6721 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6781 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6841 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6901 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     6961 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7021 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7081 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7141 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7201 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7261 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7321 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7381 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7441 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7501 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7561 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7621 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7681 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7741 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7801 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7861 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7921 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7981 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8041 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8101 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8161 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8221 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8281 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8341 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8401 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8461 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     8521 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     8581 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc
     8641 ccatgat
//
