LOCUS       sequence-425051-        8683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acetyl-CoA biosensor in the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Smith JJ, Valentino TR, Ablicki AH, Banerjee R, Colligan AR, Eckert 
            DM, Desjardins GA, Diehl KL
  TITLE     A genetically encoded fluorescent biosensor for visualization of 
            acetyl-CoA in live cells.
  JOURNAL   Cell Chem Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002.
  PUBMED    39874963
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2025 Jan 21:S2451-9456(25)00002-9. doi: 
            10.1016/j.chembiol.2025.01.002."
COMMENT     Sequence Label: Mitochondrial PancACe
FEATURES             Location/Qualifiers
     source          1..8683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      532..541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             619..642
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             655..681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    697..721
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1155..1176
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1282..1303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1543..1562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1925..2042
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2091..2601
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2520..2538
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2623..3222
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2623..2642)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3119..3139
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3350..3583
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3655..3776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3692..3711)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3746..3765
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3816..3951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3971..3990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3972..3990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4000..4017)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4000..4016)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4009..4031)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4158..4613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4245..4264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4455..4476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4639..4743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4744..4812,4813..5604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4962..4981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5775..6363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6264..6283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6517..6534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6651..6672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6687..6692,6693..6710,6711..6717)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6725..6741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6730..6752
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6749..6765
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6749..6765
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6784..6804
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6786..6804
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6832..7058
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7059..7239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7286..7411
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7904..8137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8322..8366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(8664..8683,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
      541 gtgctggcca cccgcgtgtt cagcctggtg ggcaagcgcg ccatcagcac cagcgtgtgc
      601 gtgcgcgccc acaccggtga ctacaaggat gacgatgaca agctcgatgg aggataccca
      661 tacgatgttc cagattacgc tgctcgaggt tatcaaacaa gtttgtacaa aaaagcaggc
      721 tccaccatgg ggcatatgaa gctgacaatc attcgcctgg agaaattttc cgaccaggat
      781 agaatcgacc ttcagaagat ctggcccgaa tattctccca gtagcctcca agtagacgac
      841 aaccatcgaa tctacgccgc gagatttaat gaacgactgc ttgcggcggt tagggtaacc
      901 ctcagcggta cggaaggtgc tctggactcc ctgcgggtcc gaggagcaaa cgtctatatc
      961 aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     1021 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
     1081 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac
     1141 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1201 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg cgaggagctg
     1261 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1321 agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1381 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1441 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1501 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1561 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1621 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cggtgctgag
     1681 gtgacaagac ggagaggggt aggacaatat ttgctcgagg aggtccttag aaataacccc
     1741 ggagtaagtt gctggtggat ggcggacgct ggagttgagg atcgcggagt catgacggct
     1801 ttcatgcagg cccttgggtt caccgcacag caagggggct gggagaagtg ctaatccgga
     1861 ctcagatctc gagctcaagc ttcgaattct cgacctcgag acaaatggca gtattcatcc
     1921 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     1981 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2041 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     2101 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2161 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2221 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     2281 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     2341 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     2401 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     2461 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     2521 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     2581 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
     2641 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     2701 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     2761 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     2821 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     2881 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     2941 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3001 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3061 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3121 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3181 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     3241 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     3301 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3361 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3421 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3481 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3541 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     3601 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     3661 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3721 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3781 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3841 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3901 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3961 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4021 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4081 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4141 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4201 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4261 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4321 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4381 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4441 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4501 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4561 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5881 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6541 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6601 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6661 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6721 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6781 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6841 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6901 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6961 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7021 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7261 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7321 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7381 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7441 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7501 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7561 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7621 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7681 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7741 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7801 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7861 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7921 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7981 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8041 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8101 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8161 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8221 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8281 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8341 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8401 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8461 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8521 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     8581 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     8641 ctcgagcggc cgcccccttc accgagggcc tatttcccat gat
//
