LOCUS       sequence-425053-        7230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red fluorescent, negative response-polarity voltage indicator under 
            the control of synapsin promoter; soma-targeted; for brighter 
            fluorescence .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Han Y, Yang J, Li Y, Chen Y, Ren H, Ding R, Qian W, Ren K, Xie B, 
            Deng M, Xiao Y, Chu J, Zou P
  TITLE     Bright and sensitive red voltage indicators for imaging action 
            potentials in brain slices and pancreatic islets.
  JOURNAL   Sci Adv. 2023 Nov 24;9(47):eadi4208. doi: 10.1126/sciadv.adi4208. 
            Epub 2023 Nov 22.
  PUBMED    37992174
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-11-24"; volume: "9(47):eadi4208. doi"; pages: " 
            10.1126/sciadv.adi4208. Epub 2023 Nov 22"
COMMENT     Sequence Label: pAAV-hSyn-Cepheid1b-ST-WPRE
FEATURES             Location/Qualifiers
     source          1..7230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   12..141
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        352..799
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      812..821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1022..1717
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFTWERVMNFEDGG
                     SVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKTTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYIADFKTTYKAKKPVQMPGAYNVDRKLDIVSHNEDYTVVEQYEASVGRH
                     STGGMDELYK"
     misc_feature    3374..3962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3427..3447)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3845..3856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3982..4458
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4181..4200)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4504..4644
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4504..4633
                     /label=AAV2 ITR
     rep_origin      4719..5174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4806..4825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5016..5037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5191..5210)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5310..5332
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5370..5388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5456..5560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5561..5629,5630..6421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5779..5798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6592..7180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7081..7100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgcaa agatggataa agttttaaac
      181 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
      241 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      301 ggtcggcagc aaatggttaa ttaatctaga ctgcagaggg ccctgcgtat gagtgcaagt
      361 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
      421 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
      481 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
      541 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
      601 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
      661 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
      721 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
      781 gtcgtgcctg agagcgcagt cgagaggatc cgccaccatg gctgacgtgg aaaccgagac
      841 cggcatgatt gcacagtgga ttgtctttgc tattatggct gctgctgcta ttgcttttgg
      901 agtggctgtg cactttcggc cttcagagct gaagagcgca tactatatca acattgccat
      961 ctgcactatc gccgctaccg cttactatgc aatggccgtg aactaccagg acctgacaat
     1021 gatggtgagc aagggcgagg cagtgatcaa ggagttcatg cggttcaagg tgcacatgga
     1081 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1141 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tctcctggga
     1201 catcctgtcc cctcagttca tgtacggctc cagggccttc accaagcacc ccgccgacat
     1261 ccccgactac tataagcagt ccttccccga gggcttcacc tgggagcgcg tgatgaactt
     1321 cgaggacggc ggctccgtga ccgtgaccca ggacacctcc ctggaggacg gcaccctgat
     1381 ctacaaggtg aagctccgcg gcaccaactt ccctcctgac ggccccgtaa tgcagaagac
     1441 aacaatgggc tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg
     1501 cgacattaag atggccctgc gcctgaagga cggcggccgc tacatcgcgg acttcaagac
     1561 cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt
     1621 ggacatcgtc tcccacaacg aggactacac cgtggtggaa cagtacgaag cctccgtggg
     1681 ccgccactcc accggcggca tggacgagct gtacaagaat ggtgaaaggc aggtggtcta
     1741 cgcaagatat atttgttggg tgctgaccac accactgctc ctgctcgatc tcatcgtcat
     1801 gaccaagatg ggcggagtga tgatttcttg ggtcatcggc gcagacattt tcatgatcgt
     1861 gtttggtatt ctgggcgcct tcgaggatga acacaagttc aaatgggtgt actttatcgc
     1921 tggatgtgtg atgcaggcag tcctgacata cgggatgtat aacgccactt ggaaagacga
     1981 tctgaagaaa agccccgagt accatagctc ctatgtcagt ctgctcgtct tcctgtcaat
     2041 cctctgggtg ttttatcctg tcgtgtgggc tttcgggtct ggtagtggcg tgctgtccgt
     2101 cgacaatgag gccattctca tgggaatcct ggatgtgctc gctaagccac tgtttggaat
     2161 ggggtgcctc attgcccatg agactatctt caagaccggt gccgccgacc gcccggtagt
     2221 agcagtgagc aaggcggccg ccgctagcaa atcaaggatc acctctgagg gcgagtatat
     2281 ccctctggat cagatcgaca ttaacgtcgg tggaaaatcc cgcataacct ctgagggcga
     2341 gtacatccct ctggatcaaa tcgatataaa cgtgggggga aaaagtcgaa tcacttctga
     2401 gggcgagtat atacccctcg atcagattga catcaatgtg gggggcaaca tggccatcat
     2461 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     2521 gatcgagggc gagggcgagg gccgccccta cgagggcttt cagaccgcta agctgaaggt
     2581 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcatt tcacctttgg
     2641 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttcaagc tgtccttccc
     2701 cgagggcttc aagtgggagc gcgtgatgaa ctacgaggac ggcggcgtgg tgaccgtgac
     2761 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     2821 cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctcctccga
     2881 gcggatgtac cccgaggacg gtgccctgaa gggcaagatc aagatgaggc tgaagctgaa
     2941 ggacggcggc cactacacct ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3001 gctgcccggc gcctacatcg tcgacatcaa gttggacatc acctcccaca acgaggacta
     3061 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     3121 gctgtacaag cagagccagc ctatcctgaa caccaaggag atggcccctc agagcaagcc
     3181 tcctgaggag ctggagatga gcagcatgcc tagccctgtg gcccctctgc ctgccaggac
     3241 cgagggcgtg atcgacatga ggagcatgag cagcatcgac agcttcatca gctgcgccac
     3301 cgacttccct gaggccacca ggttcttctg ctatgaaaat gaagtgtaag aattcgatat
     3361 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3781 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3901 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3961 gcatcgatac cggctcgaga cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     4021 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     4081 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     4141 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     4201 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     4261 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     4321 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     4381 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4441 gctcccttcc ctgtccttct cgagctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4501 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4561 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4621 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4681 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     4801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4921 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5041 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     5101 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5161 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5221 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5281 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5341 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5401 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5461 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5521 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5581 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5641 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5701 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5761 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5821 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5881 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5941 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6001 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6061 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6121 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6181 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6241 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6301 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6361 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6661 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6721 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6781 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6841 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6901 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6961 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7021 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7081 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7141 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7201 tttacggttc ctggcctttt gctggccttt
//
