LOCUS       sequence-42509-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Mortlock lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-Tet 129
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..116
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cgactcctat agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat
       61 ggatatctgc agaattcggc ttgaagttcc tattctctag aaagtatagg aacttcagat
      121 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
      181 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
      241 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
      301 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
      361 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
      421 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
      481 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
      541 tccctttctt ctttagcggc ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
      601 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
      661 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
      721 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
      781 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
      841 atctcaat
//
