LOCUS       sequence-425090-        5573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of jRGECO1a in striatal indirect pathway medium spiny 
            neurons (D2 MSNs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP13987:
            pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WP...
FEATURES             Location/Qualifiers
     source          1..5573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             970..987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             991..1023
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1027..1035,1036..1050)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1830..1849
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2400..2420)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2607..2718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2836..2976
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2836..2965
                     /label=AAV2 ITR
     rep_origin      3051..3506
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3138..3157)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3348..3369
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3523..3542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3642..3664
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3702..3720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3788..3892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3893..3961,3962..4753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4111..4130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4924..5512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5413..5432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgaaa aatacctagt cttgaacttt ggagttccaa
      181 attccacatg atcgacttcc tcaaggcttt tcacttgacg aacaaattgc atgccaagaa
      241 atcgtcaaaa ttctatcaag cttaattatg caatttctgt ctataaatga catacctcct
      301 gtaccctatg acacccacct atgcactaat aagatgtgac agctgcatca ccaacaaagc
      361 ctcccccgcc tggtgaaaaa tacctagtct tgaactttgg agttccaaat tccacatgat
      421 cgacttcctc aaggcttttc acttgacgaa caaattgcat gccaagaaat cgtcaaaatt
      481 ctatcaagct taattatgca atttctgtct ataaatgaca tacctcctgt accctatgac
      541 acccacctat gcactaataa gatgtgacag ctgcatcacc aacaaagcct cccccgcctg
      601 gtgaaaaata cctagtcttg aactttggag ttccaaattc cacatgatcg acttcctcaa
      661 ggcttttcac ttgacgaaca aattgcatgc caagaaatcg tcaaaattct atcaagctta
      721 attatgcaat ttctgtctat aaatgacata cctcctgtac cctatgacac ccacctatgc
      781 actaataaga tgtgacagct gcatcaccaa caaagcctcc cccgcctgga gctcgggctg
      841 ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga tccacccgcc
      901 accatgctgc agaacgagct tgctcttaag ttggctggac ttgatattaa caagactgga
      961 ggaggttctc atcatcatca tcatcatggt atggcaagca tgactggtgg acagcaaatg
     1021 ggtcgggatc tgtacgacga tgacgataag gatctcgcaa caatggtcga ctcatcacgt
     1081 cgtaagtgga ataaggcagg tcacgcagtc agagctatag gtcggctgag ctcccccgtg
     1141 gtttccgagc ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa gaaggggctg
     1201 aggctgaagg acggcggcca ctacgccgcc gaggtcaaga ccacctacaa ggccaagaag
     1261 cccgtgcagc tgcccggcgc ctacatcgtg gacatcaagt tggacatcgt gtcccacaac
     1321 gaggactaca ccatcgtgga acagtgcgaa cgcgccgagg gccgccactc caccggcggc
     1381 atggacgagc tgtacaaggg aggtacaggc gggagtctgg tgagcaaggg cgaggaggat
     1441 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1501 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
     1561 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1621 cagttcatgt acggctccaa ggcctacatt aagcacccag ccgacatccc cgactacttc
     1681 aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc
     1741 attattcacg tgaaccagga ctcctccctg caggacggcg tattcatcta caaggtgaag
     1801 ctgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1861 gaggctactc gtgacgacct gactgaagag cagatcgcag aatttaaaga ggctttctcc
     1921 ctatttgaca aggacgggga tgggacgata acaaccaagg agcttgggac ggtgttccga
     1981 tcgctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga agtagatgcc
     2041 gacggtgacg gcacattcga cttccctgag ttcctgacga tgatggcaag aaaaatgaat
     2101 gacacagaca gtgaagagga aattcgcgaa gcgttccgcg tgtttgataa ggacggcaat
     2161 ggctacatcg gcgcagcaga gcttcgccac gtgatgacag accttggaga gaagttaaca
     2221 gatgaggagg ttgatgaaat gatcagggta gcagacatcg atggggatgg tcaggtaaac
     2281 tacgaagagt ttgtacaaat gatgacagcg aagtgaggcg cgccgcggcc gcgaattcga
     2341 tatcataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2401 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2461 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     2521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2581 aattccgtgg ctcgagagat cttcgactgt gccttctagt tgccagccat ctgttgtttg
     2641 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2701 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     2761 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgaga tctcacgtgc
     2821 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     2881 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     2941 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3001 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3061 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3121 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3181 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3241 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3301 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3361 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     3421 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3481 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3541 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3601 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3661 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3721 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5041 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
