LOCUS       sequence-425100-       11451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in mammalian cells or oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen
            Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL
  TITLE     Evidence that toxin resistance in poison birds and frogs is not 
            rooted in sodium channel mutations and may rely on ""toxin sponge"" 
            proteins.
  JOURNAL   J Gen Physiol. 2021 Sep 6;153(9):e202112872. doi: 
            10.1085/jgp.202112872. Epub 2021 Aug 5.
  PUBMED    34351379
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2021-09-6"; volume: "153(9):e202112872. doi"; 
            pages: " 10.1085/jgp.202112872. Epub 2021 Aug 5"
COMMENT     Sequence Label: Pum NaV1.5
FEATURES             Location/Qualifiers
     source          1..11451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..45)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        217..596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        597..800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     751..771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     845..864
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        845..863
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7024..7041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7030..7254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7300..7728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7387..7406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7597..7618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7737..7757)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7742..8071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7922..8057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7984..8003
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8138..8932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8192..8211)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8802..8821
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9106..9227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9143..9162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9197..9216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9276..9292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9276..9292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9289..9311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9300..9316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9324..9330,9331..9348,9349..9354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9507..9524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9678..10266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9758..9777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10437..11228,11229..11297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11060..11079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11298..11402)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
       61 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      121 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      181 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      241 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      781 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      841 aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac ttaagatggc
      901 cgacttcctg ctgccccctg ggactaactc cttccaccgc tttacacccg agagcctggc
      961 tgctattgaa aaacgcatcg ctgagaagct ggccagaaat gccaagcagg agtatcggga
     1021 gcagctgggc gaggaggaga agccacagcc tcagttcgat ctgcaggcct gtaagaagct
     1081 gccagatatc tacggcaccg tgtccccaga gctgatcggc gagcctctgg aggatatcga
     1141 tcccttttat aatgacagaa agaccttcat cgtgctgaat aagggcaaga caatcttccg
     1201 gttttctgcc acccccgccc tgtatatcct gtctcctttc caccctatca gaagagccgc
     1261 catcaagatc ctggtgcact ctctgttcag catgtttatc atgtgcacaa tcctgacaaa
     1321 ctgcgtgttc atggcccagt ctgagacccc ctcttggaat aagtatgtgg agtacacctt
     1381 caccggcatc tatacattcg agtccctgat caagatcctg gccaggggct tctgcatgac
     1441 agagtttacc ttcctgaggg acccatggaa ttggctggac ttcagcgtga tcgtgatggc
     1501 ctacatcaca gagttcgtgg atctgggcaa tgtgagcgcc ctgagaacat tccgcgtgct
     1561 gagagccctg aagacaatca gcgtgatctc cggcctgaag accatcgtgg gcgccctgat
     1621 ccagtccgtg aagaagctgg ccgatgtgat gatcctgacc gtgttttgtc tgtccgtgtt
     1681 tgccctgatc ggcctgcagc tgttcatggg caacctgagg cacaagtgcg tgagagatta
     1741 tacaatgttc aactttacaa acggctccct gtatctggac ggcagaatgt ggaacaattc
     1801 cgaggagttc ctgtctgatc ctgtgaacta cttcatcaag aatggcacag aggatgtgct
     1861 gctgtgcggc aactccacag acgccggcac ctgccctgag ggctatatct gtctgaaggc
     1921 cggcgagaac ccagatcacg gctatacatc cttcgataca ttcggctggg cctttctgtc
     1981 cctgtttcgg ctgatgaccc aggattattg ggagagactg tatcagcaga ccctgcggag
     2041 cgccggcaag atctatatgc tgtttttcat gctggtaatc tttctgggct ccttttacct
     2101 ggtgaacctg atcctggccg tggtggccat ggcatatgag gagcagaacc aggccaccat
     2161 cgccgagacc gaggagaagg agagaaagtt tcgggaggcc atggagatgc tgaagaagga
     2221 gcaggaggcc ctggccgcca agggcatcga tagcatgtcc ctgtctagcc tggagatgtc
     2281 cccaagaagc gccaaggagc ggaggaataa gcggaagaag aagaagagcc tgggcgccga
     2341 tgagtatggc gaggatcaga ggaacccaag atgtgactac gatgatggcc agagaaggat
     2401 gaccctgctg ggcatctcct atggcccttc tgccaacctg gtgaagagaa gaacaagcca
     2461 cggcagcgtg ttcagctttc ggctgagagg cagagataca tgcagcgaga ccgactttgc
     2521 cgacgatgag atctccatcg ccggcgagaa tgagagccac agaggcagcc tgctgatccc
     2581 acgctctggc agaaggccaa gcgtgcagag ccaggtgtcc cactcctctc accccacaac
     2641 cccaaactac acccagaccg gcaagcggaa tagctctgtg gattgtaacg gcgtggtgtc
     2701 cctgatcggc ggcggcaccg gcgccctgaa tccagatcct acctctccag ccggcctgct
     2761 gctgccaccc gtgatcatgg agaagccagg caccggcgat ctgacctccc ccagcgacga
     2821 ggcctccaag aagcagctgc tgatgcctca caggccatcc gtggatcact ctgatgagcc
     2881 attccagagg cagagagccg tgagcgccgt gtctatcatc accagcgccc tggaggagct
     2941 ggaggagtcc caccagaagt gccccccttg ctggaaccac ttcgccgtga agtttctgat
     3001 ctgggactgt tgccccctgt ggctgctgat caagaagttt gtgaagttcg tggtaatgga
     3061 tcccttcaca gacctgacca tcaccctgtg catcgtgctg aacaccctgt tcatggccct
     3121 ggagcactat aagatgacaa aggagttcga tcacatgctg tatatcggca acctggtgtt
     3181 caccggcatc ttcaccgccg agatgatctt caaggtaatc gccctggacc cttattacta
     3241 tttccagcag ggctggaata tcttcgatag catcatcgtg atcctgtctc tgatggagct
     3301 gggcctgtct tccatgggca atctgagcgt gctgaggtct ttccggctgc tgagggtgtt
     3361 caagctggcc aagtcctggc caaccctgaa caccctgatc aagatcatcg gcaattctgt
     3421 gggcgccctg ggcaatctga ccctggtgct ggccatcatc gtgttcatct ttgccgtggt
     3481 gggcatgcag ctgttcggca agagctacct ggataacgtg aagaagatca gcacaacagg
     3541 caatctgccg cggtggcaca tgaatgactt tttccactct tttctgatca tcttccggat
     3601 cctgtgcggc gagtggatcg agacaatgtg ggactgcatg gaggtggccg gccagccact
     3661 gtgcctgctg gtgttcctgc tggtaatggt aatcggcaat ctggtggtgc tgaacctgtt
     3721 cctggccctg ctgctgagca gcttctctgc cgataacctg tccgccccag atgaggacgg
     3781 cgagatgaat aacctgcagc tggccttcgc ccgcatcaat cggggcctgc agtacatcaa
     3841 gaaggccgcc tggaattttt gctgttctgt gctgagacac ccaaagacaa cagccgagaa
     3901 gaaggccatg atgaagctgg ccgcccagaa ccctggcgcc ctgaacaatt gcgtgaacag
     3961 ccacaccacc gccgagctgg gcaaggacat ggagaaccac aaggagaacc acgccgagga
     4021 tggcgtgaac aagaatggcg agaagcaccc aggcatcacc gacgatgatt tcatgacaaa
     4081 ccccgatctg tccatctgcg tgcccatcgc cgtgggcgag agcgacatcg aggaggagga
     4141 tgaggagcag tccaccttca cagagatgga gcagcaggag aagcagctgc agctgcgggc
     4201 ccagctgtgc cagtctccag gcgtgagatc ccagaattcc gagatctatg aggagctgtc
     4261 tgagctgcac gtggataagc agctgaaggc cgagccacca ccccccgtgg gccagaagga
     4321 gctggacgag atcagcctgt ccgagggctc caccgtggac ctgaccaatc cagccgagct
     4381 gctggagcag atcccagagt tcgccgagga gctgatggag ccagaggatt gcttccccga
     4441 ggtgtgcgtg agattctttc cttgctgtag cgtggatatc accaagtttc ccggcaagat
     4501 ctggtggaga ctgcgcaaga catgttaccg gatcgtggag cacaactggt ttgagacctt
     4561 catcatcttt atgatcctgc tgtcctctgg cgccctggcc ttcgaggata tctatctgga
     4621 ggatagaaag aatatcaaga caatgctgga gtatgccgac aaggtgttca catatatctt
     4681 cgtgctggag atgctgctga agtgggtggc ctacggcttc aagaagtact tcacaaacgc
     4741 ctggtgttgg ctggactttc tgatcgtgga cgtgagcctg atcagcctgg tggccaacac
     4801 cctgggctac tccgagatgg gcccaatcaa gtccctgcgg acactgagag ccctgcggcc
     4861 cctgcgggcc ctgtccagat tcgagggcat gcgggtggtg gtgaatgccc tggtgggcgc
     4921 catcccctcc atcatgaacg tgctgctggt gtgcctgatc ttctggctga tcttttccat
     4981 catgggcgtg aatctgttcg ccggcaagtt cggcaagtgt atcaataaga cagagggcga
     5041 tatgcccctg gaccccaaga tcatcaacaa tatgtctgat tgcatcctgt acaacgtgtc
     5101 cggcacattc tattggacca aggtgaaggt gaactttgac aacgtgggcg ccgggtacct
     5161 ggccctgctg caggtggcca cattcaaggg ctggatggat atcatgtatg ccgccgtgga
     5221 ctctagagag tacgaggagc agccagagtg ggagtataac ctgtacatgt atctgtattt
     5281 cgtgatcttc atcatcttcg gctccttctt tacactgaac ctgtttatcg gcgtgatcat
     5341 cgataacttt aaccagcaga agaagaagct gggcggccag gatatcttca tgaccgagga
     5401 gcagaagaag tattacaacg ccatgaagaa gctgggcagc aagaagcccc agaagccaat
     5461 cccacggcct ctgaacaagt accagggctt catctttgac gtggtgtcta agcaggcctt
     5521 cgacgtgtcc atcatgatcc tgatctgtct gaacatggtg accatgatgg tggagacaga
     5581 tgaccagtcc caggagaagg tgaatatcct gcacaagatc aatatgctgt tcgtggccat
     5641 ctttacaggc gagtgcatct ttaagatgct ggccctgcgg cactactatt tcaccaatgg
     5701 ctggaacatc ttcgactttg tggtggtaat cctgagcatc gtgggcacag tgctgtctga
     5761 catcatccag aagtacttct tttccccaac cctgtttaga gtgatccggc tggccaggat
     5821 cggccggatc ctgaggctga tcagaggcgc caagggcatc agaaccctgc tgtttgccct
     5881 gatgatgagc ctgcccgccc tgttcaacat cggcctgctg ctgttcctgg taatgtttat
     5941 ctatgccatc ttcggcatgg ccaatttcgc ctacgtgaag aaggagcacg gcatcgacga
     6001 tatgtttaac tttcagacat tcgccaattc tatgctgtgc ctgtttcaga tcacaaccag
     6061 cgccggctgg gatggcctgc tgaatccaat cctgaatacc ggccctccat attgcgaccc
     6121 aaacctgcct aatgccaacg gctccaaggg cgattgcggc tctccagccg tgggcatcct
     6181 gttcttcgtg acctatatca tcatcagctt cctgatcgtg gtgaatatgt acatcgccat
     6241 catcctggag aatttttctg tggccaccga ggagtccacc gagcctctgt ccgaggacga
     6301 tttcgatatg ttctacgaga tctgggagaa gttcgaccct gaggccaccc agtttatcga
     6361 gtactccgcc ctgagcgatt ttgccgatgc cctgtccgag ccactgagga tcgccaagcc
     6421 aaataagatc aagctgatcg ccatggatct gcctatggtg tctggcgatc ggatccactg
     6481 cctggacatc ctgttcgcct ttacaaagag ggtgctgggc gagagcggcg agatggacgc
     6541 cctgaagatc cagatggagg agaagttcat ggccgccaac ccatctaaga tctcctacga
     6601 gcctatcacc accacactgc gcagaaagca ggaggaggtg tccgccatcg tgatccagcg
     6661 ggcctatagg agacacctgt tccggaggtc tatgaagcag gcctcctacc tgtatcggca
     6721 cagaacactg gagtcctcca tcctggagga cgacgccccc gagaaggagg gcctgatcgc
     6781 cttcatgatg aatgagaact atggccgccc tatcgataag tctgagaccc tgagcagcac
     6841 ctccttccct ccatcttatg atagcgtgac caggggcacc tctgagaatc tgcagctgaa
     6901 gatcaccgat ctgagcaaaa gcgatgaagc cattgactgt ctgctgagcc ccgataaaga
     6961 taaggaatcc attgtgtaag cggccgctcg agtctagagg gcccgtttaa acccgctgat
     7021 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7081 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7141 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7201 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7261 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     7321 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7381 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7441 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7501 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7561 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7621 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7681 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     7741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7981 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     8041 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     8101 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     8161 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     8221 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     8281 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     8341 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     8401 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     8461 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     8521 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8581 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8641 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     8701 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8761 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8821 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8881 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8941 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     9001 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9061 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9241 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10081 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11401 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct c
//
