LOCUS       sequence-425102-       12022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human zinc finger FYVE-type 
            containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) 
            promoter and mCherry under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12022)
  TITLE     Dual promoter expression vectors including protrudin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry
FEATURES             Location/Qualifiers
     source          1..12022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3783..3803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4201..4212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5251..5270
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5444..5453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5450..6160
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5595..5613)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5863..5882
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6255..6488
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6513..6531)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6514..6531)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6821..6840)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7054..7073)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7173..7195
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7233..7251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7319..7423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7424..7492,7493..8284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7642..7661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8455..9043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8944..8963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9197..9214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9284..9304)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9289..9618
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9469..9604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10752..10817
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10947..10967
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11392..11526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11429..11448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11483..11502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11694..12022,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag ctgaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat gcagacatca gaacgtgagg ggagtgggcc ggagctgagc
     2461 cccagcgtga tgcccgaggc tcccctggag tctccacctt ttcctaccaa gtccccagcg
     2521 tttgaccttt tcaacttggt tctctcctac aagaggctgg agatctacct ggaacccttg
     2581 aaggatgcag gtgatggtgt tcgatacttg ctcaggtggc agatgccttt gtgttccttg
     2641 ctgacctgcc tgggcctcaa cgtcttgttc ctcactttga atgagggtgc atggtactca
     2701 gtaggtgccc tgatgatttc agtgcccgcc ctgctgggct accttcagga ggtttgccgg
     2761 gcacggctgc ctgattccga gctgatgcgg aggaagtatc atagcgtgag gcaggaggac
     2821 ctgcagagag gtcgcctgtc tcgtcccgag gccgtggctg aggtgaagag cttcttgatc
     2881 cagctggagg ccttcctgag ccgcctgtgc tgcacatgtg aagccgccta ccgcgtgctg
     2941 cactgggaga accccgtcgt gtcctcacag ttctatgggg ctcttctggg cacagtctgc
     3001 atgctgtatt tgctgccact ctgctgggtt ctcacccttt taaacagcac gctctttctg
     3061 gggaatgtgg agttcttccg agttgtgtct gagtacaggg catctctgca gcagaggatg
     3121 aacccaaagc aggaagagca tgcctttgag agtcctccac caccagatgt tggggggaag
     3181 gatggtctga tggacagcac gcctgccctc acacccacgg agagtctctc ttcccaggac
     3241 ctcacaccgg gcagcgtgga ggaggctgag gaggctgagc cagatgaaga gtttaaagat
     3301 gcgattgagg agacccactt ggtggtgctg gaggatgatg agggcgcccc gtgcccagca
     3361 gaggatgagc tggccctgca ggacaacggg ttcctgagca agaatgaggt gctgcgcagc
     3421 aaggtgtctc ggctcacgga gcggctccgc aagcgctacc ccaccaacaa cttcgggaac
     3481 tgcacgggct gctcggccac cttctcagtg ctgaagaaga ggcggagctg cagtaattgt
     3541 ggaaacagct tctgctctcg atgctgctcc ttcaaggtgc ccaagtcctc catgggggcc
     3601 acagcccctg aagcccagag ggagactgtg tttgtgtgtg cctcgtgtaa ccagaccttg
     3661 agcaagtgaa gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3721 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4201 tcggccctca atccagcgga ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc
     4261 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     4321 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     4381 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     4441 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     4501 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     4561 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     4621 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     4681 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     4741 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     4801 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     4861 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     4921 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     4981 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     5041 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     5101 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     5161 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     5221 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     5281 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     5341 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     5401 cttttttgtt agacgaagct tggtaccgag ctcggatcaa ttcgccacca tggtgagcaa
     5461 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     5521 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5581 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5641 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5701 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5761 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     5821 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     5881 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     5941 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6001 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6061 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6121 ccgccactcc accggcggca tggacgagct gtacaagtaa ctcgagggaa ttaattcgag
     6181 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6241 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6301 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6361 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6421 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6481 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     6541 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     6601 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     6661 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     6721 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     6781 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     6841 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     6901 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     6961 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     7021 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7081 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7141 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7201 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7261 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7321 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7381 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7441 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7501 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7561 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7621 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7681 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7741 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7801 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7861 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7921 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7981 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8041 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8101 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8161 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8221 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8281 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8341 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8521 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8581 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8701 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8761 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8821 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8881 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8941 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9001 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9061 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9121 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9181 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9241 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     9301 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9361 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9421 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9481 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9541 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9601 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     9661 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     9721 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     9781 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     9841 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     9901 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     9961 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
    10021 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
    10081 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
    10141 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
    10201 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
    10261 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
    10321 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
    10381 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
    10441 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
    10501 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
    10561 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
    10621 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
    10681 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
    10741 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
    10801 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
    10861 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
    10921 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
    10981 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
    11041 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
    11101 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
    11161 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
    11221 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
    11281 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    11341 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    11401 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    11461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    11521 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    11581 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    11641 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
    11701 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
    11761 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
    11821 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
    11881 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
    11941 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
    12001 tccggagtac ttcaagaact gc
//
