LOCUS       sequence-42511-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Ferrer-Vaquer A, Piliszek A, Tian G, Aho RJ, Dufort D, Hadjantonakis
            AK
  TITLE     A sensitive and bright single-cell resolution live imaging reporter 
            of Wnt/ss-catenin signaling in the mouse.
  JOURNAL   BMC Dev Biol. 2010 Dec 21;10:121.
  PUBMED    21176145
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2010-12-21"; volume: "10"; pages: "121"
COMMENT     Sequence Label: Tcf/Lef:H2B-GFP
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..240
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      282..291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(304..681)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
ORIGIN
        1 ctcttggagt accttgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
       61 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      121 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      181 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      241 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
      301 atccttagcg ctggtgtact tggtgacggc cttagtaccc tcggacacgg cgtgcttggc
      361 caactcccca ggcagcagca ggcgcacggc cgtctggatc tccctggagg tgatggtcga
      421 gcgcttgttg taatgcgcca ggcgggaagc ctcacctgcg atgcgctcga aaatgtcgtt
      481 cacaaacgaa ttcatgatgc ccatggcctt ggacgaaatg ccggtgtcag ggtggacctg
      541 cttcagaacc ttgtacacat agatggaata gctctccttg cggctgcgct tgcgcttctt
      601 gccgcctttc ttctgcgcct tagtcaccgc cttcttggag ccctttttcg gggcgggagc
      661 agacttcgct ggctctggca tggtggcgaa ttcgaagctt gagctcgaga tctgagtccg
      721 gtagcgctag ctgtgcttct gga
//
