LOCUS       sequence-425140-       11861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 
            under the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5253
FEATURES             Location/Qualifiers
     source          1..11861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1650..2444
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      complement(2936..3391)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3073..3094)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3285..3304
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3517..3539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3531..3548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3532..3548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3558..3577
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3558..3576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4798..4826)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5421..5441
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8164..8351
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8219..8243)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            8420..9539
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        8420..8763
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             8764..9336
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      9342..9539
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(9601..9621)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9601..9619)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9640..9656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9640..9656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9653..9675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9664..9680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9688..9694,9695..9712,9713..9718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9733..9754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9871..9888)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10042..10630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10122..10141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10801..11592,11593..11661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11424..11443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11662..11766)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11834..11852
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taaccataat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg agtaaaggag aagaactttt cactggagtt
     4801 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     4861 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     4921 aaactacctg ttccatggcc aacacttgtc actactttgg gttatggtct aatgtgcttt
     4981 gctagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
     5041 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     5101 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     5161 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     5221 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     5281 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     5341 cctgtccttt taccagacaa ccattacctg tcctatcaat ctaagctttc gaaagatccc
     5401 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     5461 ggcatggatg aactatacaa aagatctatg gccactccag cggtgcaagt cactcccgat
     5521 acccttatca ccttcaaggt gagccttgat ggctcgcctc gtcgctttaa gctgcctctg
     5581 cgcgatgtcg gcatcaacgt cctcgagggc aagcttcgtg ctgccctgaa catccctccg
     5641 gaggtcgatg ccgtctttga gcgcttctcc gactctgccg ctgcctttgt cgtgctggac
     5701 caaaacaagc ctgccgtcta caagcaactc taccgtgctg ccaaggcgaa gcagaagctt
     5761 aagttgcgtg tcacaactaa gaagcccgag gaggctaaca acaagcctca gccggctgcc
     5821 gttgaggatg ctcccgagca ggaggctcag cagcctgtcg agtctgtccc tgcaccggag
     5881 cgcgaggctg actcggcaac cgccgagaac atcgcttctg cccctgagag acctgccagt
     5941 cccatgcctg aggagaagca ggtcgagatt acccaggctg ccgccgagca gagcaccacc
     6001 gagaccaaca gtgctattga gtcgttcggt gagaagctga agaccagcac agagtacctg
     6061 agagaggttc tcaacgaggc ctcgacagcg cctgctgctg ctccagagca gctgccacct
     6121 gttcctgcac cggctcccgc ggctgttcct cttccctgcc gcgttgattg cccgcctctg
     6181 cgttctcact acgctgtgtg ctgcaacaac tgcgaccgga ccattcccga tgcccactac
     6241 cactgctcga cctgtgacga tggcgacttt gacctgtgcc aggaatgtgt cgaccgcggg
     6301 gtcacctgca agggtgctga tcactggatg atcaagcgct ttgtccgcga tggcgtcatt
     6361 atcaacagca caacggagcg tcttccgccc aagacgaagc ctgctcccac ggaaaccccc
     6421 aagcctgcga cgacgtcggc gtcacctccg aggaccttcc ctaccggtcc tgcagctccc
     6481 gtcacttctg tcacccctcc tattgctcct ccggtgccga caatgacctc cgagcgtatt
     6541 atccccgttt tcaacgactt cctgtactca agcatgcgca cttgcaactg ctgtgtacgg
     6601 gacttcccgg aggtggcctt tgtccactgc actacttgcg aggactatga cctctgccgt
     6661 tcttgcttcg ccaagaaccg ccacggtcat caccccggcc atgcctttgt tcccgccgtt
     6721 gaaggcacca ggctggagcc cgaggtggct cgtctcctgg cccctggccg gggagtcatc
     6781 cacaatgccc tgtgcgatgg ctgcgataac accatccgtg gtgttcgtca caagtgcctg
     6841 aactgccctg actgggacta ctgctctcag tgtattctga atgcccctgt catccatccg
     6901 ggccaccgct tcgtgcccat ctacgagcct ctggagcagg cagacagtaa tgccaacttc
     6961 cgcttccagt ctcgtcctgt acacttcggc atttgctgcg atggcccact ttgcaataac
     7021 gggcccaaca gcaaccgata cattgttggc gaccgctaca agtgcgctgt ttgccacgac
     7081 accgacttct gcgaggcttg cgaggccagc cccgctaaca ctcataacaa gactcaccca
     7141 cttatcaagt tcaagactcc tgttcgccat gtgagcgtca ccaccaccgg tgagaacgag
     7201 catggtcgtc agatgccacc tatgggtgat cgcagggtgc cccgtcctcg tgctcccgag
     7261 tcagctcctc atcagtgccc tgctcccagc aacgttggca acgtccagac cgttgttgac
     7321 gtcaagccga ttgagccaga ggttcctgtc aaggaggaga aagttgagga gaaggtcgag
     7381 aagaagccag agccggttgt tgagaaggag ccatcggctg aagagctcga ggccactttc
     7441 gtccgcgaca ctgttcagga tggcaccgtc cttgctccca accacctctt cgagcagact
     7501 tgggttctgc gcaacacggg caaggtcgcc tggccggctg gctgctcggt caagttcgtt
     7561 ggtggcgact acatgggccg cgttgactcg gcccaccctg ctgcctcgaa ggaggttgag
     7621 gagtcttgcg agtcgaccgt ttgcgaccgc gccgtccagc ccggcgagga ggctcccttc
     7681 accgtgctcc tgcgcactcc ctaccgcgca tgccgtgtta tttcccactg gcgtctaacc
     7741 accccgaagg gtaccaagtt cggccaccgc ctgtggtgcg atgttgttgt tgagaagccc
     7801 aagtctcgtt ctccttctcc tgctcccgcc ttcgtcgagg agaaggccaa ggaagtgagc
     7861 cccgagcccg agccggccac cagccagatg atcttcccca agctggacag agagtcgccc
     7921 gttatgagcg ttcatgagga ggaggcccgg tcggagggtg ttcctacttc tgtcgcagtt
     7981 ccaagcatcc aggatgagtt tgaggactgc gaggacgtcg actggcctga aaacggctcg
     8041 gaggatgggt tccttactga tgaggagtac gacgttcttg atgcctcgga tgaggagttc
     8101 tccatggggt tgcacccgtc gacgagcaaa cagaagagat cttaacgcgc cacttctaaa
     8161 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     8221 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
     8281 tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac
     8341 acctctaccg gcagatccgc tagggataac agggtaatat gtttagcttg cctcgtcccc
     8401 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
     8461 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
     8521 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
     8581 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
     8641 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
     8701 tatacttcct ttttaaatct tgctaggata cagttctcac atcacatccg aacataaaca
     8761 accatgggta ccactcttga cgacacggct taccggtacc gcaccagtgt cccgggggac
     8821 gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg acaccgtctt ccgcgtcacc
     8881 gccaccgggg acggcttcac cctgcgggag gtgccggtgg acccgcccct gaccaaggtg
     8941 ttccccgacg acgaatcgga cgacgaatcg gacgacgggg aggacggcga cccggactct
     9001 cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg gcttcgtggt cgtctcgtac
     9061 tccggctgga accgccggct gaccgtcgag gacatcgagg tcgccccgga gcaccggggg
     9121 cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt tcgcccgcga gcggggtgcc
     9181 gggcacctct ggctggaggt caccaacgtc aacgcaccgg cgatccacgc gtaccggcgg
     9241 atggggttca ccctctgcgg cctggacacc gccctgtacg acggcaccgc ctcggacggc
     9301 gagcaggcgc tctacatgag catgccctgc ccctaatcag tactgacaat aaaaagattc
     9361 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     9421 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     9481 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     9541 tgtcgattcg atactaacgc cgccatccac ctgcaggtaa tcacgtgcca gcttttgttc
     9601 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11761 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    11821 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
