LOCUS       sequence-425187-        5039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of iCre(R297T) in striatal Sst neurons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP13777: pAAV-AiE0682h-minBG-iCre(R297T)-BGHp...
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      975..984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             987..1007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2073..2184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2302..2442
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2302..2431
                     /label=AAV2 ITR
     rep_origin      2517..2972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2604..2623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2814..2835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2989..3008)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3108..3130
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3168..3186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3254..3358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3359..3427,3428..4219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3577..3596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4390..4978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4879..4898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagacg gtctgattgc aaccctccag gaggaaaaca
      181 aattcacaaa gcagtcccag gttgccagtt ctcaaagtca gcaaaccaaa tcctaacgtg
      241 caatcttccc aggtttttca aaagccatgg gattgatagt cagtggggaa aggaagaggt
      301 gtcgaatcca ggcctgatgg atgggtgcag aggcaatcag acgtgttttc agagccactg
      361 tgctctgaca cctgccccca ttttccacag agtttggctc tgaatgcgtc cctggaccac
      421 gccctccgtc tcctttacct ggcgttccta ttcttgtaga aaggaacaca gcctaatgat
      481 ggatttgttc aatgtgatca ttctcgctta tgtacaaaac aaaggcagga ttgatgaact
      541 cctaaagaag cacattcaca ggcctgatct atgcctctta ccttggagca ggcagcaagt
      601 acagcctcca agggaatgtc aacatctttg cactttgtca ataggacctt gattctctaa
      661 aggctgaaag gtgttttcat gggtgaatgc ttagagccag ctcatggttt tccaaacccc
      721 actcaaagct tccagatgac tctttttgca tctgtgagct tctttcatcc ctagatatca
      781 ccctggggag agggcaggca aaagatgttc tctgtacttg caaggaagaa atggtaaagg
      841 catgggggtc gagtgggggc tggggactcc tatgttccgg aaagagcgag gttgagctcg
      901 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccag
      961 atctttcgaa gctagccacc atggtgccca agaagaagag gaaagtctcc aacctgctga
     1021 ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga
     1081 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     1141 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa caacaggaaa tggttccctg
     1201 ctgaacctga ggatgtgagg gactacctcc tgtacctgca agccagaggc ctggctgtga
     1261 agaccatcca acagcacctg ggccagctca acatgctgca caggagatct ggcctgcctc
     1321 gcccttctga ctccaatgct gtgtccctgg tgatgaggag aatcagaaag gagaatgtgg
     1381 atgctgggga gagagccaag caggccctgg cctttgaacg cactgacttt gaccaagtca
     1441 gatccctgat ggagaactct gacagatgcc aggacatcag gaacctggcc ttcctgggca
     1501 ttgcctacaa caccctgctg cgcattgccg aaattgccag aatcagagtg aaggacatct
     1561 cccgcaccga tggtgggaga atgctgatcc acattggcag gaccaagacc ctggtgtcca
     1621 cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa gctggtggag agatggatct
     1681 ctgtgtctgg tgtggctgat gaccccaaca actacctgtt ctgccgggtc agaaagaatg
     1741 gtgtggctgc cccttctgcc acctcccaac tgtccacccg ggccctggaa gggatctttg
     1801 aggccaccca ccgcctgatc tatggtgcca aggatgactc tgggcagaga tacctggcct
     1861 ggtctggcca ctctgccaga gtgggtgctg ccaccgacat ggccagggct ggtgtgtcca
     1921 tccctgaaat catgcaggct ggtggctgga ccaatgtgaa cattgtgatg aactacatca
     1981 gaaacctgga ctctgagact ggggccatgg tgaggctgct cgaagatggg gactgaggcg
     2041 cgccgaattc aagcttctcg agagatcttc gactgtgcct tctagttgcc agccatctgt
     2101 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     2161 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2221 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgagatctc
     2281 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     2341 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     2401 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     2461 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     2521 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2581 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2641 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2701 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     2761 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2821 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     2881 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2941 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     3001 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     3061 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3121 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     3181 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3481 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3541 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3601 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3661 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3721 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3781 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3841 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3901 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3961 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4021 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4081 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4141 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4201 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4261 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4321 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4381 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4441 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4501 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4561 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4621 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4681 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4741 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4801 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4861 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4921 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4981 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
