LOCUS       sequence-425201-       12488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PRPF4B (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12488)
  TITLE     Gateway destination clones for generating protein-proximity networks
            using proximity-dependent biotinylation proteomics
REFERENCE   2  (bases 1 to 12488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PRPF4B_IDG-K
FEATURES             Location/Qualifiers
     source          1..12488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2217..2443
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2444..2624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5403..5423)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5424..5433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5436..6206
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6213..6254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6255..6279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9310..9334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9364..9952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9417..9437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9835..9846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9966..10465
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10001..10024)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10398..10417
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10486..11085
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10486..10505)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10982..11002
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11223..11456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11528..11649
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11565..11584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11619..11638
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11689..11824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11844..11863)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11845..11863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11873..11890)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11873..11889)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11882..11904)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12031..12486
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12118..12137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12328..12349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2221 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2281 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     2341 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2401 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2641 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaact ttgtacaaaa aagcaggctc caccatggcc gccgcggaga
     6301 cccagtcgct acgggagcag ccagagatgg aagatgctaa ttctgaaaag agtataaatg
     6361 aagaaaatgg agaagtatca gaagaccagt ctcaaaataa gcacagtcgt cacaaaaaaa
     6421 agaagcataa acacagaagt aaacataaga aacataaaca ttcctcagaa gaagacaagg
     6481 ataaaaaaca taaacataag cataaacata agaaacacaa aagaaaagag gttattgatg
     6541 cttctgataa agagggtatg tctccagcaa aaagaactaa acttgatgat ttagctttgc
     6601 tagaagactt ggaaaaacag agagccttga ttaaggccga acttgataat gagttaatgg
     6661 aaggaaaggt ccagtctggt atggggctca ttttgcaagg ttatgagtct ggctctgaag
     6721 aagaggggga aattcatgaa aaggcaagaa atggaaatag gtctagtact agatcttcaa
     6781 gtacaaaggg gaaacttgaa cttgtggaca ataaaattac tacaaagaaa cgaagtaaaa
     6841 gcagatccaa agaacggact agacataggt ctgataaaaa gaaaagtaag gggggtattg
     6901 aaatcgttaa agagaaaaca actaggagca agtcaaagga gaggaaaaaa tctaaaagcc
     6961 catccaaaag aagtaagtct caagatcaag caaggaaatc aaaatcccct acccttagaa
     7021 ggcgatctca agagaaaatt ggtaaggcca gatctcctac tgatgataag gttaaaattg
     7081 aagataaaag taaatcaaaa gataggaaaa aatccccaat tataaatgaa agtagaagtc
     7141 gcgatcgagg taaaaaatcc agatccccag ttgatttaag aggtaaatcc aaagacagaa
     7201 ggtcacggtc caaagagaga aaatcaaaac ggtctgaaac tgataaagaa aagaagccaa
     7261 ttaaatctcc ctctaaagat gcttcatctg ggaaagaaaa taggtcaccc agcagaagac
     7321 ctggtcgtag tcctaaaaga agaagtttgt ctccaaaacc acgtgataaa tcaagaagaa
     7381 gcaggtctcc acttttgaat gatagaagat ctaagcagag caaatccccc tcgcggacac
     7441 tgtctcctgg gagaagagcc aagagccgat ccttagaaag aaaacgacga gaaccagaga
     7501 ggagacgact ttcttctcca agaacacgac ctcgagatga tatcctcagt agacgtgaaa
     7561 gatcaaaaga tgccagcccc atcaatagat ggtctccaac ccgaagaaga agtagatctc
     7621 ccattagaag gaggtctcgt tccccactca gacgtagcag gtctccaaga agaagaagca
     7681 gatctactcg gagaagggac agaggtcgga ggagcagatc acgcttgcga aggcggtctc
     7741 gatcacgcgg tggtcgtaga cgaaggagca gaagcaaagt aaaggaagat aaatttaaag
     7801 gaagtctttc tgaaggaatg aaagttgagc aggaatcttc gtctgatgat aaccttgaag
     7861 actttgatgt agaggaagaa gatgaagaag ccctaataga acagagaaga atccaaaggc
     7921 aggcaattgt tcagaaatat aaataccttg ctgaagatag caacatgtct gtgccatctg
     7981 aaccaagcag cccccagagc agtacgagaa cacgatcacc atctccagat gacattctgg
     8041 agcgagtagc tgctgatgtt aaagagtatg aacgggaaaa tgttgataca tttgaggcct
     8101 cagtgaaagc caagcataat ctaatgacag ttgaacagaa taatggttca tctcagaaga
     8161 agttgttggc acctgatatg tttacagaat ctgatgatat gtttgctgcg tattttgata
     8221 gtgctcgtct tcgggccgct ggcattggaa aagatttcaa agagaatccc aacctcagag
     8281 ataactggac cgatgcagaa ggctattatc gtgtgaacat aggtgaagtc ctagataaac
     8341 gttacaatgt gtatggctac actgggcaag gtgtattcag taatgttgta cgagccagag
     8401 ataatgcaag agccaaccaa gaagtggctg taaagatcat cagaaacaat gagctcatgc
     8461 aaaagactgg tttaaaagaa ttagagttct tgaaaaaact taatgatgct gatcctgatg
     8521 acaaatttca ttgtctgaga ctcttaaggc acttctatca caagcagcat ctttgtctgg
     8581 tattcgagcc tctcagcatg aacttacgag aggtgttaaa aaaatatggt aaagatgttg
     8641 gtcttcatat taaagctgta agatcctata gtcagcagtt gttcctggca ttgaaactcc
     8701 ttaaaagatg caatatccta catgcagata tcaagccaga caatatcctg gttaatgaat
     8761 ccaaaactat tttaaagctt tgcgattttg ggtcggcttc acatgttgcg gataatgaca
     8821 taacacctta tcttttcagt agattttatc gtgctcctga aatcattata ggtaaaagct
     8881 atgactatgg tatagatatg tggtctgtag gttgcacctt atacgaactc tatactggaa
     8941 aaattttatt ccctggcaaa accaataacc atatgctgaa gcttgcaatg gatctcaaag
     9001 gaaagatgcc aaataagatg attcgaaaag gtgtgttcaa agatcagcat tttgatcaaa
     9061 atctcaactt catgtacata gaagttgata aagtaacaga gagggagaaa gttactgtta
     9121 tgagcaccat taatccaact aaggacctgt tggctgactt gattgggtgc cagagacttc
     9181 ctgaagacca acgtaagaaa gtacaccagc taaaggactt gttggaccag attctgatgt
     9241 tggacccagc taaacgaatt agcatcaacc aggccctaca gcacgccttc atccaggaaa
     9301 aaatttagga cccagctttc ttgtacaaag ttgtgatatc cagcacagtg gcggccgctc
     9361 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     9661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     9721 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     9781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     9841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     9901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     9961 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
    10021 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
    10081 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
    10141 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
    10201 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
    10261 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
    10321 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
    10381 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
    10441 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
    10501 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
    10561 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
    10621 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
    10681 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
    10741 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
    10801 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
    10861 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
    10921 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
    10981 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
    11041 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
    11101 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt
    11161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
    11221 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
    11281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
    11461 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    11521 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11641 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
    11701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    11761 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    11821 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
    11881 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    11941 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    12001 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
    12061 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    12121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    12181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    12421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    12481 caatttag
//
