LOCUS       sequence-425202-       10691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of STK17A (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  TITLE     Gateway destination clones for generating protein-proximity networks
            using proximity-dependent biotinylation proteomics
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-STK17A-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(140..208,209..1000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1171..1759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1660..1679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1913..1930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2047..2068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2083..2088,2089..2106,2107..2113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2121..2137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2126..2148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2145..2161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2145..2161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2180..2200
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2182..2200
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2228..2454
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2455..2635
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2682..2807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3300..3533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3718..3762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4029..4145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4177..4197
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4197..5408
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5245..5264
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5409..5433
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6679..6703)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6705..6746
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6753..7523
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(7529..7549)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7578..8166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7631..7651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8049..8060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8180..8679
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8215..8238)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8612..8631
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8700..9299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8700..8719)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9196..9216
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9437..9670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9742..9863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9779..9798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9833..9852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9903..10038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10058..10077)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10059..10077)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10087..10104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10087..10103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10096..10118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10245..10673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10332..10351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10542..10563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      301 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      361 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1861 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1921 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1981 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2041 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2101 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2161 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     2221 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     2281 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     2341 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     2401 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     2461 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2521 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     2581 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     2641 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     2701 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     2761 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     2821 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     2881 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     2941 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     3001 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     3061 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     3121 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     3181 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     3241 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     3301 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     3361 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     3421 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     3481 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     3541 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     3601 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     3661 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     3721 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     3781 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     3841 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     3901 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     3961 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     4021 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4081 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     4141 attttatcgt ccagtgtggt ggaattctgc agatatcaac tttgtataga aaagttggcc
     4201 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     4261 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     4321 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     4381 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     4441 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     4501 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     4561 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     4621 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     4681 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     4741 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     4801 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     4861 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     4921 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     4981 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     5041 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     5101 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     5161 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     5221 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     5281 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     5341 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     5401 tgttagacgc aactttgtac aaaaaagttg gcatgatccc tttggagaag ccaggcagcg
     5461 gcggctcctc cccaggcgcc acctcaggct cgggccgggc aggccggggt ctgagcgggc
     5521 cgtgccggtc gccgccgccg ccccaggccc gcgggctgtt gacagagata cgcgccgtgg
     5581 tgcgcaccga gcccttccag gacggctaca gcctgtgccc gggccgggag ctgggcaggg
     5641 ggaaatttgc agtggtgaga aaatgtataa agaaagattc tgggaaagaa tttgctgcaa
     5701 agttcatgag aaaaagaaga aaaggccaag attgtcggat ggaaataatt catgagattg
     5761 ctgtacttga actagcacaa gacaatcctt gggtcattaa tttacatgaa gtttatgaga
     5821 ctgcatcaga aatgatctta gttctggaat atgctgctgg gggtgaaatc tttgaccagt
     5881 gtgttgcaga cagagaagaa gcctttaaag aaaaagatgt tcaaagactt atgcgacaga
     5941 ttttagaagg tgttcacttt ttacacactc gtgatgtagt tcatcttgat ttgaagcctc
     6001 agaatattct gttgacaagt gaatctccat tgggtgacat taagattgtt gattttggcc
     6061 tttcaagaat attgaagaac agtgaagagc tccgagaaat tatgggtacc cctgaatatg
     6121 tggctcctga aattcttagt tatgatccta taagcatggc aacagatatg tggagcattg
     6181 gagtgttaac atatgtcatg cttacaggaa tatcaccttt cttaggcaat gataaacaag
     6241 aaacattctt aaacatctca cagatgaatt taagttattc tgaggaagaa tttgatgttt
     6301 tgtctgagtc ggctgttgat ttcatcagga cacttttagt taagaaacct gaagatcgag
     6361 ccactgctga agaatgtcta aagcacccct ggttgacaca gagcagtatt caagagcctt
     6421 ctttcaggat ggaaaaggca ctagaagaag caaatgccct ccaagaaggt cattctgtgc
     6481 ctgaaattaa ttcggatacc gacaaatcag aaaccgagga atccattgta accgaagagt
     6541 taattgtagt tacttcatat actctaggac aatgcagaca gtctgaaaaa gagaaaatgg
     6601 agcaaaaggc catttccaaa cgatttaaat ttgaggaacc tttgctacaa gaaattccag
     6661 gagaatttat ctacgtttac ccaactttct tgtacaaagt gggcggcaag cccatcccca
     6721 accccctgct gggcctggac agcaccgcta gcatcccgct gctgaacgct aaacagattc
     6781 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
     6841 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
     6901 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
     6961 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
     7021 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
     7081 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
     7141 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
     7201 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
     7261 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
     7321 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
     7381 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
     7441 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
     7501 tcagtctgcg gtctgccgaa aagtaagcca actttattat acatagttga tatccagcac
     7561 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7981 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8161 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     8221 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     8281 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     8341 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     8401 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     8461 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     8521 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     8581 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     8641 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     8701 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8761 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8821 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8881 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     8941 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     9001 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     9061 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     9121 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     9181 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     9241 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     9301 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     9361 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9421 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     9481 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9541 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9601 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9661 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9721 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     9781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9841 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     9901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10021 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
    10081 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10141 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10201 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
    10261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    10321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    10381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    10501 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10561 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10621 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    10681 aatattaacg c
//
