LOCUS       sequence-425207-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pHis5 backbone integrating plasmid expressing Pil1-mCherry under 
            the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5228
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1188..1838
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     rep_origin      complement(2272..2727)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2409..2430)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2621..2640
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2853..2875
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2867..2884
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2868..2884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2894..2913
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2894..2912
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5163..5870
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5308..5326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5576..5595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      5898..6085
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5953..5977)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6154..7510
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6154..6497
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6498..7307
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6565..6584)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7313..7510
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7567..7587)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7567..7585)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7606..7622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7606..7622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7619..7641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7630..7646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7654..7660,7661..7678,7679..7684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7837..7854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8008..8596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8088..8107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8767..9558,9559..9627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9390..9409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9628..9732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9800..9818
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggagc ggccgctcct caattcgatg ttgcagattt tacaagtttt taaaatgtat
      721 ttcattatta ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt
      781 ttttccagtg cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg
      841 aaaagttaat atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat
      901 atcgttgaat atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt
      961 aaggatatct tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat
     1021 tggcttgctg gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct
     1081 tgcaatttca cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc
     1141 ctgtaattct cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt
     1201 ttgtagaaag aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc
     1261 ccttacctga agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa
     1321 agggagaaca agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg
     1381 cactggctaa acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg
     1441 atgatcatca cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta
     1501 tgggtaactt tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc
     1561 tttctagaag cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa
     1621 agcgtgaaaa ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt
     1681 cggtagcagc tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc
     1741 gtgctgaaag cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg
     1801 gaagttctga agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg
     1861 aaaagaacga caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag
     1921 aaccttactt gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta
     1981 cgtagccgta taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca
     2041 atgcgtcgtc acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt
     2101 atggtaaaat taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac
     2161 ttttaagtaa cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta
     2221 gggcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2281 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2341 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2521 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2701 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     2761 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2821 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     2881 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgatcagaa
     2941 aattatcgcc ataaaagaca gaataagtca tcagcggttg tttcatttcc tatatttttt
     3001 ttttattttt ttatttttta ataagggaaa atttaacgtc taaggataca gaagattgtt
     3061 agcacattaa agtaataaag gcttaagtag taagtgcctt agcatgttat tgtatttcaa
     3121 aggacataat ctaaaataat aacaatatca tttctcacaa gttattcaat tttctttttt
     3181 ttttctaata atatcaagaa tgtattattt gtttgacata agtcaactaa tttatttaat
     3241 atgctggatt aatcttgcag acatgtaaat taacaagttt tagtcaaata acgttgaagt
     3301 ttcaatgaac tcaaataatt tctctttttt tttatataac cataatctga tttatatttt
     3361 ccgcagggat caactgaagt tatgacattt ggattggatc acttataacc ttggtcgcca
     3421 aataatacaa aaatcagcgt tataaaacaa agaaggtttt tgttaagaaa ttaatcctct
     3481 ttcttgataa gaaagttgaa ccgaaattgc agatactgat atatgaaaat aatacccaca
     3541 attttgggaa tagcgcaagc ctcaatttaa acaataggtg aggacacatg ataatgacct
     3601 caatgattgt tagaagaaaa gagcctcatt acaaaatcga aaaatgaatg gttgggtaca
     3661 agtttccaaa acatggtaaa gtggactttg cgtatgagac gtaaatagaa aaaaacactt
     3721 gttatatgtt ttctagaatt attgttgtct ctttatggtt ggatgatgca aaatagtaat
     3781 ttcggttagt tgctgtaaaa caccacgaga caaatagata tggatattta ttaaatcagg
     3841 aaaaacgtaa ctctcggcta ctggatggtt cagtcaccca acgattactg gggagagaaa
     3901 acagggcaaa agcaaagctt aaaggaatcc gattgtcatt cggcaatgtg cagcgaaact
     3961 aaaaaccgga taatggacct gttaatcgaa acattgaaga taaaggaaga ggaatcctgg
     4021 catatcatca attgaataag ttgaattaat tatttcaatc tcattctcac tttctgactt
     4081 atagtcgctt tgttaaagct agcatgatga accgtgcgta ctctttaaga aacagccgtg
     4141 cccctacggc ctcacaattg ttaaaccccc ctcctccctc ctcttccact cgtagtggtc
     4201 gtttgtttgc ccccttgagt cataccatgc gtatcaacca cgctgccacc ttcacccctg
     4261 acttggctaa gcgtttggct gtcttggtca agatggaaaa gaacgtgatg cgcagtatgg
     4321 aggtcaccat ccgtggccgt cgtgattgtg ctcgccaact ttcctactgg ggtgaggact
     4381 gcgatgacga tatcagcgac gtcaccgaca aattgggtgt cttgttttac gagatggccg
     4441 agttggagaa ctacttgatc gatcgttatg accaataccg tatgacgttg aagtccatcc
     4501 gtaacatcga gtcctctgtc caaccctctc gtgagaagaa gcaaaagctc ttggatcaaa
     4561 tctatgctct taagcataag gatcctgaat cccctcgctt ggtcaccatg gagcaagagc
     4621 ttgttcgtga ggaggctgcc tgtttggttg ccgaggctca actttccaac accacccgtg
     4681 aaaagttcaa gcaagccatg accttcaact tggatgccct ccacgagcat gccgagaagc
     4741 tcaacctcat tgccacctat ggcagacatt tgttgaactt gattgacgac acccccgtca
     4801 ctcccggtga ggctcgtcct gcctacgatg gttatgagac ttcccgccaa atcgtcatgg
     4861 atgctgagca cgccttgagc tcttgggttc ccagccaacc cgccgtcaac tttgtcaagc
     4921 ctcaaatcga ggatgatgcc caaagtgacg ctcgttcctg gaacgagtat gaggctcaag
     4981 gtgagcccgt ccccgttgag caagtcaccc acttacaaga cgacgttcaa tccgacacta
     5041 gcgagatcat tgagaacgag cctggcatgc accctcatgt ccatgctgag cgtatgtctc
     5101 gcattgcttc tgagtctagc gatgccgttc ctcagcaaac cgccgtccag gttgctgcta
     5161 gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     5221 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     5281 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     5341 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     5401 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5461 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     5521 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     5581 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     5641 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     5701 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     5761 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5821 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag agatcttaac
     5881 gcgccacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
     5941 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     6001 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg
     6061 ctcttattga ccacacctct accggcagat ccgctaggga taacagggta atatgtttag
     6121 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
     6181 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     6241 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     6301 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
     6361 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     6421 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     6481 tccgaacata aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt
     6541 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     6601 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     6661 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     6721 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     6781 tcaccactgc gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag
     6841 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     6901 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     6961 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     7021 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     7081 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     7141 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     7201 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     7261 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa
     7321 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     7381 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     7441 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     7501 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtttaaacga gctccagctt
     7561 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     7621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7681 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7741 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7801 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7861 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     9781 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
