LOCUS       sequence-425209-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pLys3 backbone integrating plasmid expressing Pil1-mCherry under 
            the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5227
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2536..2991)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2673..2694)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2885..2904
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3117..3139
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3131..3148
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3132..3148
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3158..3177
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3158..3176
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5427..6134
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5572..5590)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5840..5859
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      6162..6349
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6217..6241)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        6418..6761
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7160..7357
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7419..7439)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7419..7437)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7458..7474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7458..7474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7471..7493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7482..7498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7506..7512,7513..7530,7531..7536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7551..7572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7689..7706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7860..8448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7940..7959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8619..9410,9411..9479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9242..9261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9480..9584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9652..9670
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg gggccttacg aagttaaatt ataggcacag acatctttgg cattggaaag
      241 atttagtaac aatcataatg tagcacttct taaaaaatct aattttagaa caaaaaggct
      301 taaaaaagaa tggttccgct cttttaaaaa atagtaaaat gtacaatagc aaggttttcg
      361 taggtcgacg gacataacaa ggggaaagag aaataaagca tgtaaacgtc aataaaacat
      421 ctttgattct ggatcaatca tatgccaaat gaagaaaaat agcatttata acaacatgaa
      481 accatatttg agacctttta acgatgaaaa tttattaaat aaaagataaa aaagaaagaa
      541 accgttcaag ttcaatgaaa atttcgtcgt gaagtagaaa ccaagataat ggaaaggctt
      601 cgcaaagcca caaattttcc aatagtcatt ataactgaag attaaggaca gtaagcggcc
      661 gcttactagt aaaaatcaag tgaattgtgc ctaggatagg tagctaacgt gactgccact
      721 gaacctgtgt agcgtattag atccatcaag atcccttctg ctcatatcat tgctgatctg
      781 taaattgacc gcatcaaatt tgatctcatt tatatcgaga ttgatctata ttattttgcc
      841 aatttgcagt atataaactt atttgattaa gtttttggtc tccttcgcca cgtcttcatt
      901 ttgttagttt aaattttttt gactaactta gaaagggaag tttttaaaaa cgttgaaata
      961 ctttttcgat ttaaaaatat atatttaatt aattttctgt tccaaaatgg ttgctcctca
     1021 tctttggtta cgtgctgaaa ccaaaccttt agaagagcgc agtgctttga ctcctagaac
     1081 tgccaagata ctcgccgatg ctggatttca aattaccatt gagcgctctt cccaacgtgc
     1141 tttcaaggac aaagaatttg agcgccttgg tttccccatg gttcctgagg gctcatggcg
     1201 acatgcacct aaggacgcgt acattattgg tctcaaagag cttccggaaa atgataacag
     1261 tcctttgaag catactcata ttcaatttgc tcattgctac aaaaaccagg aaggttggcg
     1321 tgaagtcttg tcccgattcc ccgctggcaa tggtcttcta tatgatttgg aatttttgca
     1381 agatgacaat ggcagacgtg ttgccgcttt tggttaccac gctggttttg ctggatcagc
     1441 tatctcatgt ttagtttggg cacatcaatt attgcatcct aacaaacaat tccctgctat
     1501 ccgtccgttc cccaacgaaa aatctttggt tcgccatgtt gctcgtcaag tacgtttagc
     1561 tttgaaaaag aataacaacc agtatcctcg tattctggtt attggggctc ttggtcgttg
     1621 tggtactgga gcgtgcgatt tagcatcaaa gattggtatt cccttcgata acattcttcg
     1681 ctgggacatt aatgaaacta agaagggtgg tccctttacc gaaattacgg aatcagacat
     1741 atttgttaac tgcatttatc tgagcatgcc tattcccaaa ttctgtaccg tcgagtcgtt
     1801 aaatgtacct aaccgtaaat tgcgtgttgt ttgtgatgtt agctgtgaca ctaccaatcc
     1861 taacaatcct attccaattt ataatgtaaa cactacattt gatcatccta cagttgaggt
     1921 taaaggtgtc acaactcctc ctcctttaga ggtcatttct attgatcatc ttcctacttt
     1981 attgccaagg gaatcttccg aagcatttag tgaagctcta attcctagtc ttttggcttt
     2041 gaaggacgtc gacaatgctc ccgtttgggt cagagctaaa aaactttatg aaaccatggt
     2101 tcaaaagctt taaaataaat gttatttaat ttcaaagttc cgggtccttt atattgtttc
     2161 accaaattat tgaatattat tttctcaaaa attaacttta gaatacaatt tagacggcat
     2221 tttcgatgct ctggacattt tttccacgta cttgttaaat tgtagcggtg ttgcataata
     2281 ctaccttttg atttgttatt cacgttacaa tatatatttg actaatttaa atatgataat
     2341 atacacattt ctctgcaaac ctcctttata cttttcgccg gtcattgatt aataattaag
     2401 aaacaggatt tcatcctgca tttgccactt acctatatgg taaatataaa tcttgaaact
     2461 ttccccaata tttattgctt gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga
     2521 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2581 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2641 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2701 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2761 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2821 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2881 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2941 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     3001 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3061 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3121 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     3181 attgggtacc gggcccgatc agaaaattat cgccataaaa gacagaataa gtcatcagcg
     3241 gttgtttcat ttcctatatt ttttttttat ttttttattt tttaataagg gaaaatttaa
     3301 cgtctaagga tacagaagat tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg
     3361 ccttagcatg ttattgtatt tcaaaggaca taatctaaaa taataacaat atcatttctc
     3421 acaagttatt caattttctt ttttttttct aataatatca agaatgtatt atttgtttga
     3481 cataagtcaa ctaatttatt taatatgctg gattaatctt gcagacatgt aaattaacaa
     3541 gttttagtca aataacgttg aagtttcaat gaactcaaat aatttctctt tttttttata
     3601 taaccataat ctgatttata ttttccgcag ggatcaactg aagttatgac atttggattg
     3661 gatcacttat aaccttggtc gccaaataat acaaaaatca gcgttataaa acaaagaagg
     3721 tttttgttaa gaaattaatc ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac
     3781 tgatatatga aaataatacc cacaattttg ggaatagcgc aagcctcaat ttaaacaata
     3841 ggtgaggaca catgataatg acctcaatga ttgttagaag aaaagagcct cattacaaaa
     3901 tcgaaaaatg aatggttggg tacaagtttc caaaacatgg taaagtggac tttgcgtatg
     3961 agacgtaaat agaaaaaaac acttgttata tgttttctag aattattgtt gtctctttat
     4021 ggttggatga tgcaaaatag taatttcggt tagttgctgt aaaacaccac gagacaaata
     4081 gatatggata tttattaaat caggaaaaac gtaactctcg gctactggat ggttcagtca
     4141 cccaacgatt actggggaga gaaaacaggg caaaagcaaa gcttaaagga atccgattgt
     4201 cattcggcaa tgtgcagcga aactaaaaac cggataatgg acctgttaat cgaaacattg
     4261 aagataaagg aagaggaatc ctggcatatc atcaattgaa taagttgaat taattatttc
     4321 aatctcattc tcactttctg acttatagtc gctttgttaa agctagcatg atgaaccgtg
     4381 cgtactcttt aagaaacagc cgtgccccta cggcctcaca attgttaaac ccccctcctc
     4441 cctcctcttc cactcgtagt ggtcgtttgt ttgccccctt gagtcatacc atgcgtatca
     4501 accacgctgc caccttcacc cctgacttgg ctaagcgttt ggctgtcttg gtcaagatgg
     4561 aaaagaacgt gatgcgcagt atggaggtca ccatccgtgg ccgtcgtgat tgtgctcgcc
     4621 aactttccta ctggggtgag gactgcgatg acgatatcag cgacgtcacc gacaaattgg
     4681 gtgtcttgtt ttacgagatg gccgagttgg agaactactt gatcgatcgt tatgaccaat
     4741 accgtatgac gttgaagtcc atccgtaaca tcgagtcctc tgtccaaccc tctcgtgaga
     4801 agaagcaaaa gctcttggat caaatctatg ctcttaagca taaggatcct gaatcccctc
     4861 gcttggtcac catggagcaa gagcttgttc gtgaggaggc tgcctgtttg gttgccgagg
     4921 ctcaactttc caacaccacc cgtgaaaagt tcaagcaagc catgaccttc aacttggatg
     4981 ccctccacga gcatgccgag aagctcaacc tcattgccac ctatggcaga catttgttga
     5041 acttgattga cgacaccccc gtcactcccg gtgaggctcg tcctgcctac gatggttatg
     5101 agacttcccg ccaaatcgtc atggatgctg agcacgcctt gagctcttgg gttcccagcc
     5161 aacccgccgt caactttgtc aagcctcaaa tcgaggatga tgcccaaagt gacgctcgtt
     5221 cctggaacga gtatgaggct caaggtgagc ccgtccccgt tgagcaagtc acccacttac
     5281 aagacgacgt tcaatccgac actagcgaga tcattgagaa cgagcctggc atgcaccctc
     5341 atgtccatgc tgagcgtatg tctcgcattg cttctgagtc tagcgatgcc gttcctcagc
     5401 aaaccgccgt ccaggttgct gctagcatgg tgagcaaggg cgaggaggat aacatggcca
     5461 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     5521 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     5581 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     5641 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     5701 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     5761 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     5821 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5881 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5941 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     6001 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     6061 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     6121 acgagctgta caagagatct taacgcgcca cttctaaata agcgaatttc ttatgattta
     6181 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     6241 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     6301 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
     6361 gggataacag ggtaatatgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     6421 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     6481 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     6541 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
     6601 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     6661 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     6721 ctaggataca gttctcacat cacatccgaa cataaacaac catgcctttg tctcaagaag
     6781 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     6841 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     6901 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     6961 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     7021 cctgcggacg gtgtcgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     7081 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     7141 tgtgggaggg ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     7201 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     7261 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     7321 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     7381 ccatccacct gcaggtaatc acgtgccagc ttttgttccc tttagtgagg gttaattgcg
     7441 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7501 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7561 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7621 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8281 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9601 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     9661 atcacgaggc cctttcgtc
//
