LOCUS       sequence-425213-       10486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pAde6 backbone integrating plasmid expressing Pil1-mCherry under 
            the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5226
FEATURES             Location/Qualifiers
     source          1..10486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5598..6305
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5743..5761)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6011..6030
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      6333..6520
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6388..6412)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6589..8164
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6589..6932
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6933..7961
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      7967..8164
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8226..8246)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8226..8244)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8265..8281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8265..8281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8278..8300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8289..8305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8313..8319,8320..8337,8338..8343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8358..8379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8496..8513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8667..9255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8747..8766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9426..10217,10218..10286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10049..10068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10287..10391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10459..10477
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     3361 cgggcccgat cagaaaatta tcgccataaa agacagaata agtcatcagc ggttgtttca
     3421 tttcctatat ttttttttta tttttttatt ttttaataag ggaaaattta acgtctaagg
     3481 atacagaaga ttgttagcac attaaagtaa taaaggctta agtagtaagt gccttagcat
     3541 gttattgtat ttcaaaggac ataatctaaa ataataacaa tatcatttct cacaagttat
     3601 tcaattttct tttttttttc taataatatc aagaatgtat tatttgtttg acataagtca
     3661 actaatttat ttaatatgct ggattaatct tgcagacatg taaattaaca agttttagtc
     3721 aaataacgtt gaagtttcaa tgaactcaaa taatttctct ttttttttat ataaccataa
     3781 tctgatttat attttccgca gggatcaact gaagttatga catttggatt ggatcactta
     3841 taaccttggt cgccaaataa tacaaaaatc agcgttataa aacaaagaag gtttttgtta
     3901 agaaattaat cctctttctt gataagaaag ttgaaccgaa attgcagata ctgatatatg
     3961 aaaataatac ccacaatttt gggaatagcg caagcctcaa tttaaacaat aggtgaggac
     4021 acatgataat gacctcaatg attgttagaa gaaaagagcc tcattacaaa atcgaaaaat
     4081 gaatggttgg gtacaagttt ccaaaacatg gtaaagtgga ctttgcgtat gagacgtaaa
     4141 tagaaaaaaa cacttgttat atgttttcta gaattattgt tgtctcttta tggttggatg
     4201 atgcaaaata gtaatttcgg ttagttgctg taaaacacca cgagacaaat agatatggat
     4261 atttattaaa tcaggaaaaa cgtaactctc ggctactgga tggttcagtc acccaacgat
     4321 tactggggag agaaaacagg gcaaaagcaa agcttaaagg aatccgattg tcattcggca
     4381 atgtgcagcg aaactaaaaa ccggataatg gacctgttaa tcgaaacatt gaagataaag
     4441 gaagaggaat cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt
     4501 ctcactttct gacttatagt cgctttgtta aagctagcat gatgaaccgt gcgtactctt
     4561 taagaaacag ccgtgcccct acggcctcac aattgttaaa cccccctcct ccctcctctt
     4621 ccactcgtag tggtcgtttg tttgccccct tgagtcatac catgcgtatc aaccacgctg
     4681 ccaccttcac ccctgacttg gctaagcgtt tggctgtctt ggtcaagatg gaaaagaacg
     4741 tgatgcgcag tatggaggtc accatccgtg gccgtcgtga ttgtgctcgc caactttcct
     4801 actggggtga ggactgcgat gacgatatca gcgacgtcac cgacaaattg ggtgtcttgt
     4861 tttacgagat ggccgagttg gagaactact tgatcgatcg ttatgaccaa taccgtatga
     4921 cgttgaagtc catccgtaac atcgagtcct ctgtccaacc ctctcgtgag aagaagcaaa
     4981 agctcttgga tcaaatctat gctcttaagc ataaggatcc tgaatcccct cgcttggtca
     5041 ccatggagca agagcttgtt cgtgaggagg ctgcctgttt ggttgccgag gctcaacttt
     5101 ccaacaccac ccgtgaaaag ttcaagcaag ccatgacctt caacttggat gccctccacg
     5161 agcatgccga gaagctcaac ctcattgcca cctatggcag acatttgttg aacttgattg
     5221 acgacacccc cgtcactccc ggtgaggctc gtcctgccta cgatggttat gagacttccc
     5281 gccaaatcgt catggatgct gagcacgcct tgagctcttg ggttcccagc caacccgccg
     5341 tcaactttgt caagcctcaa atcgaggatg atgcccaaag tgacgctcgt tcctggaacg
     5401 agtatgaggc tcaaggtgag cccgtccccg ttgagcaagt cacccactta caagacgacg
     5461 ttcaatccga cactagcgag atcattgaga acgagcctgg catgcaccct catgtccatg
     5521 ctgagcgtat gtctcgcatt gcttctgagt ctagcgatgc cgttcctcag caaaccgccg
     5581 tccaggttgc tgctagcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     5641 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5701 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5761 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5821 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5881 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5941 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6001 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6061 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6121 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6181 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6241 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6301 acaagagatc ttaacgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     6361 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     6421 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     6481 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
     6541 gggtaatatg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     6601 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     6661 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     6721 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcgga cctgcgagca gggaaacgct
     6781 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     6841 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     6901 agttctcaca tcacatccga acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga
     6961 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcggctct
     7021 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     7081 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     7141 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     7201 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     7261 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     7321 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     7381 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     7441 ccgtcggtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     7501 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7561 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7621 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7681 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7741 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7801 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7861 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7921 gaaaccgacg ccccagcact cgtccgaggg caaaggaata atcagtactg acaataaaaa
     7981 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     8041 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     8101 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     8161 actgctgtcg attcgatact aacgccgcca tccacctgca ggtaatcacg tgccagcttt
     8221 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     8281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10441 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
