LOCUS       sequence-425215-       10259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pUra4 backbone integrating plasmid expressing Pil1-mCherry under 
            the inducible promoter 41nmt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Pan ZQ, Yang YS, Du LL
  TITLE     Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in 
            the Fission Yeast Schizosaccharomyces pombe.
  JOURNAL   Methods Mol Biol. 2025;2862:93-102. doi: 
            10.1007/978-1-0716-4168-2_7.
  PUBMED    39527195
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2025"; volume: "2862:93-102. doi"; pages: " 
            10.1007/978-1-0716-4168-2_7"
COMMENT     Sequence Label: pDB5225
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1650..2444
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      complement(2936..3391)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3073..3094)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3285..3304
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3517..3539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3531..3548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3532..3548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3558..3577
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3558..3576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5827..6534
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5972..5990)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6240..6259
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      6562..6749
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6617..6641)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6818..7937
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        6818..7161
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7162..7734
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      7740..7937
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7999..8019)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7999..8017)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8038..8054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8038..8054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8051..8073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8062..8078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8086..8092,8093..8110,8111..8116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8131..8152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8269..8286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8440..9028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8520..8539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9199..9990,9991..10059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9822..9841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10060..10164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10232..10250
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taaccataat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg atgaaccgtg cgtactcttt aagaaacagc
     4801 cgtgccccta cggcctcaca attgttaaac ccccctcctc cctcctcttc cactcgtagt
     4861 ggtcgtttgt ttgccccctt gagtcatacc atgcgtatca accacgctgc caccttcacc
     4921 cctgacttgg ctaagcgttt ggctgtcttg gtcaagatgg aaaagaacgt gatgcgcagt
     4981 atggaggtca ccatccgtgg ccgtcgtgat tgtgctcgcc aactttccta ctggggtgag
     5041 gactgcgatg acgatatcag cgacgtcacc gacaaattgg gtgtcttgtt ttacgagatg
     5101 gccgagttgg agaactactt gatcgatcgt tatgaccaat accgtatgac gttgaagtcc
     5161 atccgtaaca tcgagtcctc tgtccaaccc tctcgtgaga agaagcaaaa gctcttggat
     5221 caaatctatg ctcttaagca taaggatcct gaatcccctc gcttggtcac catggagcaa
     5281 gagcttgttc gtgaggaggc tgcctgtttg gttgccgagg ctcaactttc caacaccacc
     5341 cgtgaaaagt tcaagcaagc catgaccttc aacttggatg ccctccacga gcatgccgag
     5401 aagctcaacc tcattgccac ctatggcaga catttgttga acttgattga cgacaccccc
     5461 gtcactcccg gtgaggctcg tcctgcctac gatggttatg agacttcccg ccaaatcgtc
     5521 atggatgctg agcacgcctt gagctcttgg gttcccagcc aacccgccgt caactttgtc
     5581 aagcctcaaa tcgaggatga tgcccaaagt gacgctcgtt cctggaacga gtatgaggct
     5641 caaggtgagc ccgtccccgt tgagcaagtc acccacttac aagacgacgt tcaatccgac
     5701 actagcgaga tcattgagaa cgagcctggc atgcaccctc atgtccatgc tgagcgtatg
     5761 tctcgcattg cttctgagtc tagcgatgcc gttcctcagc aaaccgccgt ccaggttgct
     5821 gctagcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     5881 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     5941 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     6001 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     6061 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     6121 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     6181 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     6241 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     6301 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     6361 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     6421 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     6481 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagatct
     6541 taacgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     6601 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     6661 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     6721 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatgt
     6781 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     6841 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     6901 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     6961 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     7021 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     7081 actgaggttc ttctttcata tacttccttt ttaaatcttg ctaggataca gttctcacat
     7141 cacatccgaa cataaacaac catgggtacc actcttgacg acacggctta ccggtaccgc
     7201 accagtgtcc cgggggacgc cgaggccatc gaggcactgg atgggtcctt caccaccgac
     7261 accgtcttcc gcgtcaccgc caccggggac ggcttcaccc tgcgggaggt gccggtggac
     7321 ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg acgaatcgga cgacggggag
     7381 gacggcgacc cggactctcg gacgttcgtc gcgtacgggg acgacggcga cctggcgggc
     7441 ttcgtggtcg tctcgtactc cggctggaac cgccggctga ccgtcgagga catcgaggtc
     7501 gccccggagc accgggggca cggggtcggg cgcgcgttga tggggctcgc gacggagttc
     7561 gcccgcgagc ggggtgccgg gcacctctgg ctggaggtca ccaacgtcaa cgcaccggcg
     7621 atccacgcgt accggcggat ggggttcacc ctctgcggcc tggacaccgc cctgtacgac
     7681 ggcaccgcct cggacggcga gcaggcgctc tacatgagca tgccctgccc ctaatcagta
     7741 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     7801 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     7861 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     7921 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccacct gcaggtaatc
     7981 acgtgccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     8041 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8101 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8281 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8341 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8401 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8461 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8521 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8581 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8641 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8701 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8761 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8821 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8881 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8941 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9001 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9061 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9121 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9181 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9241 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9301 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9361 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9421 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9481 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9541 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9601 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9661 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9721 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9781 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9841 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9901 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9961 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10021 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10081 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10141 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10201 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
