LOCUS       sequence-425221-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing scramble of shRNA targeting Cdh10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-scrCdh10.1 GFP
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(89..109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(507..518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(627..643)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..644)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             833..1066
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1138..1259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1175..1194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1229..1248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1299..1434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1454..1473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1455..1473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1483..1500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1483..1499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1492..1514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1641..2096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1728..1747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1938..1959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2122..2226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2227..2295,2296..3087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2445..2464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3258..3846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3747..3766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4000..4017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4134..4155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4170..4175,4176..4193,4194..4200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4208..4224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4213..4235
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4232..4248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4232..4248
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4267..4287
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4269..4287
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4315..4541
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4542..4722
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4769..4894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5387..5620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5805..5849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6147..6395
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6147..6167
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6318..6337
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6492..6609
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6976..7279
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7281..7556
                     /label=chicken beta-actin promoter
     intron          7557..8573
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8497..8519
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8581..8600
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      8650..8659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8656..8658,8659..8661,8662..9375)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8656..8658,8659..8661,8662..9372)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8701..8722)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8962..8981)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9309..9330
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9395..9428)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
       61 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
      481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      601 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
      661 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
      721 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
      781 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      841 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      901 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      961 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1021 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     1081 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     1141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1261 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1381 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     1441 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     1501 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1561 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1621 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     1681 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     1741 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     1801 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     1861 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1921 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1981 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2041 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     2101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4021 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4081 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4141 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4201 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     4261 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     4321 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     4381 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     4441 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     4501 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     4561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     4741 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     4801 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     4861 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     4921 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     4981 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     5041 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     5101 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     5161 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     5221 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     5281 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5341 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5401 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5461 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5521 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5581 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     5641 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     5701 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     5761 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     5821 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     5881 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     5941 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     6001 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     6061 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     6121 agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     6181 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     6241 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     6301 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     6361 tggctttata tatcttgtgg aaaggacgaa acaccgggga cattaacgaa taatacagct
     6421 cgagctgtat tattcgttaa tgtccttttt gaattctcga cctcgagaca aatggcagta
     6481 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6541 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6601 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg
     6661 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     6721 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     6781 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     6841 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     6901 agccgcacgt ctcactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6961 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     7021 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     7081 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     7141 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7201 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     7261 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     7321 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     7381 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
     7441 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     7501 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     7561 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
     7621 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
     7681 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
     7741 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
     7801 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
     7861 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     7921 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     7981 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     8041 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     8101 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     8161 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     8221 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     8281 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     8341 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     8401 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     8461 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     8521 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     8581 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgcg gccgctagcg
     8641 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     8701 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     8761 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     8821 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     8881 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     8941 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     9001 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     9061 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     9121 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     9181 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     9241 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     9301 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9361 gagctgtaca agtaggaatt cgtcgaggga cctaataact tcgtatagca tacattatac
     9421 gaagttatac atgtttaagg gttccggt
//
