LOCUS       sequence-425224-        9107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base plasmid for dual-luciferase constructs to measure terminator 
            strength.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Gorjifard S, Jores T, Tonnies J, Mueth NA, Bubb K, Wrightsman T, 
            Buckler ES, Fields S, Cuperus JT, Queitsch C
  TITLE     Arabidopsis and maize terminator strength is determined by GC 
            content, polyadenylation motifs and cleavage probability.
  JOURNAL   Nat Commun. 2024 Jul 12;15(1):5868. doi: 10.1038/s41467-024-50174-7.
  PUBMED    38997252
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-07-12"; volume: "15(1):5868. doi"; pages: " 
            10.1038/s41467-024-50174-7"
COMMENT     Sequence Label: pDLterm
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1902..2154)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2174..2725)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2767..2946)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     3178..3200
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3192..3209
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3193..3209
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3219..3238
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3219..3237
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3262..3278
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3263..3279
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3479..3525
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             3553..4068
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4170..4190)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4170..4188)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4209..4225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4209..4225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4222..4244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4233..4249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4257..4263,4264..4281,4282..4287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4302..4323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4440..4457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4562..4586
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4677..5265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4757..4776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5436..6224)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNGTLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6515..6950
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    7078..7100
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             complement(7318..8970)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     8886..8904
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atctatgaaa ccctaatcga gaattaagat gatatctaac gatcaaaccc agaaaatcgt
       61 cttcgatcta agattaacag aatctaaacc aaagaacata tacgaaattg ggatcgaacg
      121 aaaacaaaat cgaagatttt gagagaataa ggaacacaga aatttacctt gatcacggta
      181 gagagaattg agagaaagtt tttaagattt tgagaaattg aaatctgaat tgtgaagaag
      241 aagagctctt tgggtattgt tttatagaag aagaagaaga aaagacgagg acgactaggt
      301 cacgagaaag ctaaggcggt gaagcaatag ctaataataa aatgacacgt gtattgagcg
      361 ttgtttacac gcaaagttgt ttttggctaa ttgccttatt tttaggttga ggaaaagtat
      421 ttgtgctttg agttgataaa cacgactcgt gtgtgccggc tgcaaccact ttgacgccgt
      481 ttattactga ctcgtcgaca accacaattt ctaacggtcg tcataagatc cagccgttga
      541 gatttaacga tcgttacgat ttatattttt ttagcattat cgttttattt tttaaatata
      601 cggtggagct gaaaattggc aataattgaa ccgtgggtcc cactgcattg aagcgtattt
      661 cgtattttct agaattcttc gtgctttatt tcttttcctt tttgtttttt tttgccattt
      721 atctaatgca agtgggctta taaaatcagt gaatttcttg gaaaagtaac ttctttatcg
      781 tataacatat tgtgaaatta tccatttctt ttaatttttt agtgttattg gatatttttg
      841 tatgattatt gatttgcata ggataatgac ttttgtatca agttggtgaa caagtctcgt
      901 taaaaaaggc aagtggtttg gtgactcgat ttattcttgt tatttaattc atatatcaat
      961 ggatcttatt tggggcctgg tccatattta acactcgtgt tcagtccaat gaccaataat
     1021 attttttcat taataacaat gtaacaagaa tgatacacaa aacattcttt gaataagttc
     1081 gctatgaaga agggaactta tccggtccta gatcatcagt tcatacaaac ctccatagag
     1141 ttcaacatct taaacaagaa tatcctgatc tgaagaatgt ggaggcttta gtcccttgga
     1201 tacttgggag gctgtggaag aacagaaacg agctggtgct taaagggagg gaatttggaa
     1261 ccaatgaggt attagtaagg acacaagaag atgcagatga gtggattaga aggaaagagg
     1321 ctcagaatgt aaggaaagca ccaaccacaa caccgtcggg acagacgaga aacatgggaa
     1381 gctccaccac aatcttgggt taagtgcaac tttgatgggg catggccaac agaaggatta
     1441 aaatgtggct tagggtgggt gcttcgcgat catacaggga aggtgttatg gttaggtgca
     1501 agagctgtgg taaaagtaag aagcgtgctg gaagtagaag tggaggctct tagatgggct
     1561 gtgctgtcat tatcccgatt caattatagg aagatcattt ttgaggtgga ttctcagcaa
     1621 cttgtgtctt tggttacatg aaagttatgc ttgtcaagtc tcaatccaat tatccaagac
     1681 ataaagtatc tacttagcaa gtttgaggat tttatgcttg tgcatacaag ccgagaagga
     1741 aatggagtgg cagatagaat agctaaggaa tctctttctt ttgagaatta tgatctaaag
     1801 ttgtattcta ttgtaccaat ttgggttaaa agctctgttg agctagactg tgtatccata
     1861 aatgggtgaa tggaaagttt gttgttgagc gcatgccggt cgatctagta acatagatga
     1921 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
     1981 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
     2041 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
     2101 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgttttaac gatctgcttg
     2161 actctagggg tcatcagatt tcggtgacgg gcaggaccgg acggggcggc accggcaggc
     2221 tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc
     2281 gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg
     2341 gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt
     2401 gggtgtagag cgtggagccc agtcccgtcc gctggtggcg tggagatacg tacacggtgg
     2461 actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg acccgcgtag gcgatgccgg
     2521 cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt
     2581 cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga
     2641 tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt
     2701 cggccgggcg tcgttctggg ctcatggtag atcccctcga tcgagttgag agtgaatatg
     2761 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     2821 aatatttgct agcttatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     2881 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     2941 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     3001 gtgactccct taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc
     3061 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     3121 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     3181 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     3241 aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc
     3301 actcggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag gtggaccccc
     3361 acccacgagg agcatcgtgg aaaacgaagt cgttccaacc acggcttcaa agcaagtgga
     3421 tggatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcctgtgc
     3481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tggaattcta
     3541 gtatactaaa ccatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc
     3601 ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc
     3661 ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc
     3721 gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa
     3781 aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat
     3841 ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat
     3901 gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc
     3961 aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc
     4021 atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgtaagg gagacgggat
     4081 cctacagcta gccgtctcga ggtgttccac ttctgtatag gagccaattg attgacaaca
     4141 tgcatcaatc ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4201 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4381 taatgaatcg gccaacgctc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4441 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4501 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgaaggcc
     4561 ttgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg
     4621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4801 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4861 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4921 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4981 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5041 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5101 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5161 ggaagaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5221 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5281 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatt
     5341 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5401 atctaaagta tatatgtgta acattggtct agtgattatt tgccgactac cttggtgatc
     5461 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     5521 tcttgtccaa gataagcctg cctagcttca agtatgacgg gctgatactg ggccggcagg
     5581 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     5641 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     5701 gagttccata gcgttaaggt tccatttagc gcctcaaata gatcctgttc aggaaccgga
     5761 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     5821 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     5881 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     5941 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     6001 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     6061 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     6121 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     6181 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcataacacc ccttgtatta
     6241 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     6301 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     6361 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     6421 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact
     6481 ttctggctgg atgatggggc gattcaggcg atccccatcc aacagcccgc cgtcgagcgg
     6541 gcttttttat ccccggaagc ctgtggatag agggtagtta tccacgtgaa accgctaatg
     6601 ccccgcaaag ccttgattca cggggctttc cggcccgctc caaaaactat ccacgtgaaa
     6661 tcgctaatca gggtacgtga aatcgctaat cggagtacgt gaaatcgcta ataaggtcac
     6721 gtgaaatcgc taatcaaaaa ggcacgtgag aacgctaata gccctttcag atcaacagct
     6781 tgcaaacacc cctcgctccg gcaagtagtt acagcaagta gtatgttcaa ttagcttttc
     6841 aattatgaat atatatatca attattggtc gcccttggct tgtggacaat gcgctacgcg
     6901 caccggctcc gcccgtggac aaccgcaagc ggttgcccac cgtcgagcgc ctttgcccac
     6961 aacccggcgg ccggccgcaa cagatcgttt tataaatttt ttttttttga aaaagaaaaa
     7021 gcccgaaagg cggcaacctc tcgggcttct ggatttccga tccccggaat tagatcttgg
     7081 caggatatat tgtggtgtaa cgttatcagc ttatctggat tttagtactg gattttggtt
     7141 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
     7201 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
     7261 actactcaca cattattatg gagaaactcg agcttgtcga tcgactctag ctagagctta
     7321 cacggcgatc tttccgccct tcttggcctt tatgaggatc tctctgattt ttcttgcgtc
     7381 gagttttccg gtaagacctt tcggtacttc gtccacaaac acaactcctc cgcgcaactt
     7441 tttcgcggtt gttacttgac tggcgacgta atccacgatc tctttttccg tcatcgtctt
     7501 tccgtgctcc aaaacaacaa cggcggcggg aagttcaccg gcgtcatcgt cgggaagacc
     7561 tgcgacacct gcgtcgaaga tgttggggtg ttggagcaag atggattcca attcagcggg
     7621 agccacctga tagcctttgt acttaatcag agacttcagg cggtcaacga tgaagaagtg
     7681 ttcgtcttcg tcccagtaag ctatgtctcc agaatgtagc catccatcct tgtcaatcaa
     7741 ggcgttggtc gcttccggat tgtttacata accggacata atcataggac ctctcacaca
     7801 cagttcgcct ctttgattaa cgcccagcgt tttcccggta tccagatcca caaccttcgc
     7861 ttcaaaaaat ggaacaactt taccgaccgc gcccggttta tcatccccct cgggtgtaat
     7921 cagaatagct gatgtagtct cagtgagccc atatccttgc ctgatacctg gcagatggaa
     7981 cctcttggca accgcttccc cgacttcctt agagagggga gcgccaccag aagcaatttc
     8041 gtgtaaatta gataaatcgt atttgtcaat cagagtgctt ttggcgaaga aggagaatag
     8101 ggttggcacc agcagcgcac tttgaatctt gtaatcctga aggctcctca gaaacagctc
     8161 ttcttcaaat ctatacatta agacgactcg aaatccacat atcaaatatc cgagtgtagt
     8221 aaacattcca aaaccgtgat ggaatggaac aacacttaaa atcgcagtat ccggaatgat
     8281 ttgattgcca aaaataggat ctctggcatg cgagaatctc acgcaggcag ttctatgagg
     8341 cagagcgaca cctttaggca gaccagtaga tccagaggag ttcatgatca gtgcaattgt
     8401 cttgtcccta tcgaaggact ctggcacaaa atcgtattca ttaaaaccgg gaggtagatg
     8461 agatgtgacg aacgtgtaca tcgactgaaa tccctggtaa tccgttttag aatccatgat
     8521 aataattttt tggatgattg ggagcttttt ttgcacgttc aaaatttttt gcaacccctt
     8581 tttggaaacg aacaccacgg taggctgcga aatgcccata ctgttgagca attcacgttc
     8641 attataaatg tcgttcgcgg gcgcaactgc aactccgata aataacgcgc ccaacaccgg
     8701 cataaagaat tgaagagagt tttcactgca tacgacgatt ctgtgatttg tattcagccc
     8761 atatcgtttc atagcttctg ccaaccgaac ggacatttcg aagtactcag cgtaagtgat
     8821 gtccacctcg atatgtgcat ctgtaaaagc aattgttcca ggaaccaggg cgtatctctt
     8881 catagcctta tgcagttgct ctccagcggt tccatcttcc agcggataga atggcgccgg
     8941 gcctttcttt atgtttttgg cgtcttccat ctgttaatca gaaaaactca gattaatcga
     9001 caaattcgat cgcacaaact agaaactaac accagatcta gatagaaatc acaaatcgaa
     9061 gagtaattat tcgacaaaac tcaaattatt tgaacaaatc ggatgat
//
