LOCUS       sequence-425229-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing scramble of shRNA targeting Cdh20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-scrCdh20.1 GFP
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    773..898
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             945..1125
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1126..1352)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1380..1400)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1380..1398)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1419..1435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1419..1435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1432..1454)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1443..1459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1467..1473,1474..1491,1492..1497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1512..1533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1650..1667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1821..2409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1901..1920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2580..3371,3372..3440))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3203..3222
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3441..3545)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3571..4026)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3708..3729)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3920..3939
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4153..4175
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4167..4184
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4168..4184
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4194..4213
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4194..4212
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4233..4368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4419..4438)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4473..4492
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4601..4834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     5023..5039
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5024..5040
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5043..5631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5149..5160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5558..5578
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    5687..5720
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(5740..6453,6454..6456,6457..6459))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5743..6453,6454..6456,6457..6459))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5785..5806)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6134..6153
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6393..6414
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6456..6465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6515..6534)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6542..7558
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6596..6618)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7559..7835)
                     /label=chicken beta-actin promoter
     enhancer        7837..8140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8507..8624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(8721..8969)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8779..8798)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8949..8969)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(9267..9311)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
       61 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
      121 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
      181 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
      241 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc
      301 tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac
      361 tactttatat ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct
      421 gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
      481 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
      541 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
      601 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
      661 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
      721 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
      781 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
      841 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
      901 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
      961 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     1021 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     1081 gccagagagc tcccaggctc agatctggtc taaccagaga gacccgttta tgtatcgagc
     1141 taggcactta aatacaatat ctctgcaatg cggcaattca gtggttcgtc caatccatgt
     1201 cagacccgtc tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg
     1261 gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt
     1321 gcaagactac aagagtattg cataagacta cattaagctt gcagctccag cttttgttcc
     1381 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     1441 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1501 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1561 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1621 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2221 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2461 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2521 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2581 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2641 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2701 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2761 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2821 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2881 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2941 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3001 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3061 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3121 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3181 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3241 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3301 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3361 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3421 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3481 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3541 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     3601 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3661 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3721 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3781 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3841 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3901 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3961 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4021 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4081 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     4141 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     4201 actcactata gggcgaattg ggtacgtccc taggcctcca aaaaagcctc ctcactactt
     4261 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     4321 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     4381 gggcgggata gctagagcca gacatgataa gatacattga tgagtttgga caaaccacaa
     4441 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4501 taaccattat aagctgcaat aaacaagttc ctctcactct ctgatattca tttctttgca
     4561 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
     4621 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     4681 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     4741 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     4801 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     4861 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gtacctgagg tgtgactgga
     4921 aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt ggtagctgct
     4981 gtattgctac ttgtgattgc tccatgtttt tctaggtctc gatcgaggtc gacggtatcg
     5041 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     5101 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     5161 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     5221 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     5281 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     5341 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     5401 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     5461 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     5521 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
     5581 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
     5641 cttgatatcg aattgtacct agtggaaccg gaacccttaa acatgtataa cttcgtataa
     5701 tgtatgctat acgaagttat taggtccctc gacgaattcc tacttgtaca gctcgtccat
     5761 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     5821 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     5881 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     5941 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
     6001 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     6061 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     6121 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     6181 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     6241 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
     6301 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     6361 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     6421 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag
     6481 cggccgcgaa ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga
     6541 gctgtaggaa aaagaagaag gcatgaacat ggttagcaga ggctctagag ccgccggtca
     6601 cacgccagaa gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccccgcg
     6661 gacagccccg aggctggaga gggagaaggg gacggcggcg cggcgacgca cgaaggccct
     6721 ccccgcccat ttccttcctg ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg
     6781 tgcggcggcg cctcccagat ttcggctccg cacagatttg ggacaaagga agtccctgcg
     6841 ccctctcgca cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc
     6901 cggcgctccg ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg
     6961 ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac
     7021 gccccgtacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt
     7081 gcaggggggg ttgcagcccg accgacgcgc ccacaccccc tgctcacccc cccacgcaca
     7141 caccccgcac gcagcctttg ttcccctcgc agcccccccc gcaccgcggg gcaccgcccc
     7201 cggccgcgct cccctcgcgc acactgcgga gcgcacaaag ccccgcgccg cgcccgcagc
     7261 gctcacagcc gccgggcagc gcggagccgc acgcggcgct ccccacgcac acacacacgc
     7321 acgcaccccc cgagccgctc cccccgcaca aagggccctc ccggagcccc tcaaggcttt
     7381 cacgcagcca cagaaaagaa acaagccgtc attaaaccaa gcgctaatta cagcccggag
     7441 gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg
     7501 ggcggcgcga ggcggcggcg gagcggggca cggggcgaag gcagcgcgca gcgactcccg
     7561 cccgccgcgc gcttcgcttt ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa
     7621 actttcggag cgcgccgctc tgattggctg ccgccgcacc tctccgcctc gccccgcccc
     7681 gcccctcgcc ccgccccgcc ccgcctggcg cgcgcccccc cccccccccg cccccatcgc
     7741 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggagat
     7801 ggggagagtg aagcagaacg tggggctcac ctcgaccatg gtaatagcga tgactaatac
     7861 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     7921 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     7981 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     8041 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     8101 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     8161 atgaactaat gaccccgtaa ttgattacta ttaataacta gtgagacgtg cggcttccgt
     8221 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
     8281 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
     8341 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
     8401 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccct
     8461 cgagccgcgg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     8521 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     8581 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     8641 ccatttgtct cgaggtcgag aattcaaaaa gtcgtgtaca cgtcaaggag tctcgagact
     8701 ccttgacgtg tacacgaccc ggtgtttcgt cctttccaca agatatataa agccaagaaa
     8761 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
     8821 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
     8881 gtgtttacag tcaaattaat tccaattatc tctctaacag ccttgtatcg tatatgcaaa
     8941 tatgaaggaa tcatgggaaa taggccctcg gtgaaggggg cggccgctcg aggctagtct
     9001 cgtgatcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     9061 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     9121 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     9181 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     9241 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     9301 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     9361 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     9421 gatttattac tccaactagc attccaagg
//
