LOCUS       sequence-425230-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for inducible knockdown of human ARNTL(BMAL1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Halawani D, Wang Y, Ramakrishnan A, Estill M, He X, Shen L, Friedel 
            RH, Zou H
  TITLE     Circadian clock regulator Bmal1 gates axon regeneration via Tet3 
            epigenetics in mouse sensory neurons.
  JOURNAL   Nat Commun. 2023 Aug 24;14(1):5165. doi: 10.1038/s41467-023-40816-7.
  PUBMED    37620297
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-24"; volume: "14(1):5165. doi"; pages: " 
            10.1038/s41467-023-40816-7"
COMMENT     Sequence Label: pLKO-Tet-On-ARNTL-shRNA1
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    94..327
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             512..556
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        845..939
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     856..875
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    893..911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    921..939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1039..1156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1205..1715
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1634..1652
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1724..2296
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2304..2323
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2351..2369
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2380..3000
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2453..2472)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    3035..3608
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3202..3219)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3429..3448
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3628..4227
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3628..3647)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4124..4144
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4355..4588
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4660..4781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4697..4716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4751..4770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4821..4956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4977..4995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5005..5022)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5005..5021)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5014..5036)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5163..5618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5250..5269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5460..5481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5644..5748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5749..5817,5818..6609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5967..5986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6780..7368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7269..7288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7522..7539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7656..7677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7692..7697,7698..7715,7716..7722)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7730..7746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7735..7757
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7754..7770
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7754..7770
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7789..7809
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7791..7809
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7837..8063
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8064..8244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8291..8416
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
       61 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
      121 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
      181 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
      241 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
      301 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
      361 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
      421 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
      481 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
      541 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
      601 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
      661 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
      721 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
      781 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
      841 cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatccctatc
      901 agtgatagag acttataagt tccctatcag tgatagagac accggcttct aggcacatcg
      961 tgttatctcg agataacacg atgtgcctag aagtttttaa ttctcgacct cgagacaaat
     1021 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     1081 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     1141 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
     1201 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     1261 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     1321 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     1381 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     1441 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     1501 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     1561 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     1621 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     1681 ccctcgttga ccgaatcacc gacctctctc cccaggggga tctgtgagtt tggggaccct
     1741 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
     1801 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
     1861 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
     1921 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
     1981 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
     2041 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     2101 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     2161 ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     2221 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     2281 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     2341 caaagaattg taatacgact cactataggg cgaattgata tgtctagatt agataaaagt
     2401 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     2461 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     2521 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     2581 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     2641 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     2701 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     2761 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     2821 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     2881 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     2941 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
     3001 gcgtacagcg gctcccggga gttctaggga tctgcccctc tccctccccc ccccctaacg
     3061 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     3121 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     3181 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     3241 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     3301 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     3361 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     3421 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     3481 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     3541 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     3601 gatgataagg atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     3661 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3721 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     3781 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3841 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3901 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     3961 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4021 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4081 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4141 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4201 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     4261 aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta
     4321 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4381 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4561 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     4621 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     4681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     4981 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5041 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5101 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5161 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5581 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     5641 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5701 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5761 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5821 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5881 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5941 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6001 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6061 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6121 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6181 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6241 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6301 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6361 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6421 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6481 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6541 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6601 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6661 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6721 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6781 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6841 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6901 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6961 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7021 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7141 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7201 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7381 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7441 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7501 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7561 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7621 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7681 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7741 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     7801 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     7861 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     7921 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     7981 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     8041 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     8101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8221 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8281 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8341 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8401 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8461 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8521 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8581 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8641 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8701 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8761 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttata
//
