LOCUS       sequence-425253-        8652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A)
            for induction of global DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Hosseinpour M, Xi X, Liu L, Malaver-Ortega L, Perlaza-Jimenez L, Joo
            JE, York HM, Beesley J, Caldon CE, Dugue PA, Dowty JG, Arumugam S, 
            Southey MC, Rosenbluh J
  TITLE     SAM-DNMT3A, a strategy for induction of genome-wide DNA methylation,
            identifies DNA methylation as a vulnerability in ER-positive breast 
            cancers.
  JOURNAL   Nat Commun. 2024 Dec 1;15(1):10449. doi: 10.1038/s41467-024-54824-8.
  PUBMED    39617792
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-1"; volume: "15(1):10449. doi"; pages: " 
            10.1038/s41467-024-54824-8"
COMMENT     Sequence Label: SAM-DNMT3A-inactiveEmpty
FEATURES             Location/Qualifiers
     source          1..8652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1717..1965
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1888..1907
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2239..2356
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2405..2915
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2834..2852
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3380..3404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3454..3474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4402..4455
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4483..5079
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4483..4502)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4979..4999
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5169..5402
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5480..5601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5517..5536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5571..5590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5641..5776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5796..5815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5797..5815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5825..5842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5825..5841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5834..5856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5983..6438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6070..6089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6280..6301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6464..6568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6569..6637,6638..7429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6787..6806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7600..8188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8089..8108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8342..8359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8476..8497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8512..8517,8518..8535,8536..8542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8550..8566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8555..8577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8574..8590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8574..8590
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8609..8629
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8611..8629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga cgaccggttt
     1981 tttgcgtctc tgtttaagag ctatgctgcg aatacgaggg agcagacgat atggcgtcgc
     2041 tcctctccac gagagcatat gggctccgtg gtctcgtaca ccatcagggt acgtatcaga
     2101 caccatcagg gtctgctcgt attcgcagca tagcaagttt aaataaggct agtccgttat
     2161 caacttgaaa aagtggcacc gagtcggtgc tttttttgaa ttctagatct tgagacaaat
     2221 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2281 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2341 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     2401 cgagggggcc cgggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     2461 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     2521 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     2581 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     2641 aaacggaagc cgcacgtctt actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     2701 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     2761 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     2821 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     2881 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaacttaa gcttgccacc
     2941 atgtcgcgga ggcggcacag ctacgagaac gatggtggac aacctcacaa aaggaggaag
     3001 acgtctatgc tagcctccaa aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg
     3061 actgagatcc agtccaccgc agaccgtcag atcttcgaag agaaggtcgg gcctctggtg
     3121 ggtcggctgc gcctcacggc ttcgctccgt caaaacggag ccaagaccgc gtatcgagtc
     3181 aacctaaaac tggatcaggc ggacgtcgtt gattccggac ttccgaaagt gcgctacact
     3241 caggtatggt cgcacgacgt gacaatcgtt gcgaatagca ccgaggcctc gcgcaaatcg
     3301 ttgtacgatt tgaccaagtc cctcgtcgcg acctcgcagg tcgaagatct tgtcgtcaac
     3361 cttgtgccgc tgggccgttc ttcaactttc ttgtacaaag tgggcggagg aggtggaagc
     3421 ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc
     3481 gctaaccacg accaggaatt tgaccctcca aaggtttacc cacctgtccc agctgagaag
     3541 aggaagccca tccgggtgct gtctctcttt gatggaatcg ctacagggct cctggtgctg
     3601 aaggacttgg gcattcaggt ggaccgctac attgcctcgg aggtgtgtga ggactccatc
     3661 acggtgggca tggtgcggca ccaggggaag atcatgtacg tcggggacgt ccgcagcgtc
     3721 acacagaagc atatccagga gtggggccca ttcgatctgg tgattggggg cagtccctgc
     3781 aatgacctct ccatcgtcaa ccctgctcgc aagggcctct acgagggcac tggccggctc
     3841 ttctttgagt tctaccgcct cctgcatgat gcgcggccca aggagggaga tgatcgcccc
     3901 ttcttctggc tctttgccaa tgtggtggcc atgggcgtta gtgacaagag ggacatctcg
     3961 cgatttctcg agtccaaccc tgtgatgatt gatgccaaag aagtgtcagc tgcacacagg
     4021 gcccgctact tctggggtaa ccttcccggt atgaacaggc cgttggcatc cactgtgaat
     4081 gataagctgg agctgcagga gtgtctggag catggcagga tagccaagtt cagcaaagtg
     4141 aggaccatta ctacgaggtc aaactccata aagcagggca aagaccagca ttttcctgtc
     4201 ttcatgaatg agaaagagga catcttatgg tgcactgaaa tggaaagggt atttggtttc
     4261 ccagtccact atactgacgt gtccaacatg agccgcttgg cgaggcagag actgctgggc
     4321 cggtcatgga gcgtgccagt catccgccac ctcttcgctc cgctgaagga gtattttgcg
     4381 tgtgtgtgta caggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     4441 gagaatcctg gcccaggtac cacagatcca ccggagctta ccatgaccga gtacaagccc
     4501 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4561 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4621 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4681 gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4741 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4801 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4861 accgtcggcg tttcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4921 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     4981 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5041 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg ctagctgaac gcgttaagtc
     5101 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5161 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5221 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5281 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5341 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5401 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5461 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5521 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5581 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5641 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5701 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5761 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5821 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5881 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5941 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     6001 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6061 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6421 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8341 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8401 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8461 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8521 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8581 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8641 ggagctgcaa gc
//
