LOCUS       sequence-425270-        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1) Ectopic expression of MAAP protein variant in mammalian cells. 2)
            Packaging MAAP variant sequence into lentivirus for stable 
            expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer
            DV
  TITLE     Evolving membrane-associated accessory protein variants for improved
            adeno-associated virus production.
  JOURNAL   Mol Ther. 2024 Feb 7;32(2):340-351. doi: 
            10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18.
  PUBMED    38115579
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2023.12.015"; journalName: "Mol Ther"; date: 
            "2024-02-7- 7"; volume: "32"; issue: "2"; pages: "340-351"
COMMENT     Sequence Label: pCW57-CMV-MAAP-SL08V9
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1972..1983
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctggagcccc tcaacccaag gcaaatcaac aacatcaaga caacgctcga ggtcttgtgc
       61 ttccgggtta caaatacctt ggacccggca acggactcga caagggggag ccggtcaacg
      121 cagcagacgc ggcggccctc gagcacgaca aggcctacga ccagcagctc aaggccggag
      181 acaacccgta cctcaagtac aaccacgccg acgccgagtt ccaggagcgg ctcaaagaag
      241 atacgtcttt tgggggcaac ctcgggcgag cagtcttcca ggccaaaaag aggcttcttg
      301 cctcttggtc tggttgagga agcggctaag acggctcctg gaaagaagag gcctgtagag
      361 cagtctcctc aggaaccgga ctcctccgcg ggtattggca aatcgggtgc acagcccgct
      421 aaaaagagac tcaatttcgg tcagactggc gacacagagt cagtcccaga ccctcaacca
      481 atcggagaac ctcccgcagc cccctcaggt gtgggatctc ttacaatggc ttcaggtggt
      541 ggcgcaccag tggcagacaa taacgaaggt gccgatggag tgggtagttc ctcgggaaat
      601 tggcattgcg attcccaatg gctgggggac agagtcatca ccaccagcac ccgaacctgg
      661 gccctgccca cctacaacaa tcacctctac aagcaaatct ccaacagcac atctggagga
      721 tcttcaaatg acaacgccta cttcggctac agcaccccct gggggtattt tgacttcaac
      781 agattccact gccacttctc accacgtgac tggcagcgac tcatcaacaa caactgggga
      841 ttccggccta agcgactcaa cttcaagctc ttcaacattc aggtcaaaga ggttacggac
      901 aacaatggag tcaagaccat cgccaataac cttaccagca cggtccaggt cttcacggac
      961 tcagactatc agctcccgta cgtgctcggg tcggctcacg agggctgcct cccgccgttc
     1021 ccagcggacg ttttcatgat tcctcagtac gggtatctga cgcttaatga tggaagccag
     1081 gccgtgggtc gttcgtcctt ttactgcctg gaatatttcc cgtcgcaaat gctaagaacg
     1141 ggtaacaact tccagttcag ctacgagttt gagaacgtac ctttccatag cagctacgct
     1201 cacagccaaa gcctggaccg actaatgaat ccactcatcg accaatactt gtactatctc
     1261 tcaaagacta ttaacggttc tggacagaat caacaaacgc taaaattcag tgtggccgga
     1321 cccagcaaca tggctgtcca gggaagaaac tacatacctg gacccagcta ccgacaacaa
     1381 cgtgtctcaa ccactgtgac tcaaaacaac aacagcgaat ttgcttggcc tggagcttct
     1441 tcttgggctc tcaatggacg taatagcttg atgaatcctg gacctgctat ggccagccac
     1501 aaagaaggag aggaccgttt ctttcctttg tctggatctt taatttttgg caaacaagga
     1561 actggaagag acaacgtgga tgcggacaaa gtcatgataa ccaacgaaga agaaattaaa
     1621 actactaacc cggtagcaac ggagtcctat ggacaagtgg ccacaaacca ccagagtgcc
     1681 caagcacagg cgcagaccgg ctgggttcaa aaccaaggaa tacttccggg tatggtttgg
     1741 caggacagag atgtgtacct gcaaggaccc atttgggcca aaattcctca cacggacggc
     1801 aactttcacc cttctccgct gatgggaggg tttggaatga agcacccgcc tcctcagatc
     1861 ctcatcaaaa acacacctgt acctgcggat cctccaacgg ccttcaacaa ggacaagctg
     1921 aactctttca tcacccagta ttctactggc caagtcagcg tggagatcga gtgggagctg
     1981 cagaaggaaa acagcaagcg ctggaacccg gagatccagt acacttccaa ctattacaag
     2041 tctaataatg ttgaatttgc tgttaatact gaaggtgtat atagtgaacc ccgccccatt
     2101 ggcaccagat acctgactcg taatctgtaa
//
