LOCUS       sequence-425295-        9822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of ETV2 for endothelial cell 
            differentiation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Zhao Y, Wang K
  TITLE     Kai Wang Lab Plasmids January 2024
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBAN-iETV2-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1385..1763
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1393..1411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1429..1447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1465..1483
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1501..1519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1537..1555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1573..1591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1609..1627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1672..1696
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1789..1798
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(2842..3416,3417..3419,3420..3428)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3009..3026)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3236..3255
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3429..4139
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3574..3592)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3842..3861
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4146..4163)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4152..4359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4436..4647
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4660..4928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4962..5705
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5706..5759
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5760..6359
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5760..5779)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6256..6276
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6392..6513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6429..6448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6483..6502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7276..7310
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7801..7817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7801..7817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7814..7836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7825..7841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7849..7855,7856..7873,7874..7879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7894..7915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8032..8049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8203..8791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8283..8302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8962..9753,9754..9822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9585..9604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt
     1381 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1441 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1501 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1561 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1621 atagagaacg tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
     1681 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc
     1741 gtaccacttc ctaccctcgt aaaggtctag agctagcggc gcgccgttgc caccatggat
     1801 ttgtggaatt gggatgaggc atcacctcaa gaggttccgc ctggtaacaa acttgcaggg
     1861 ctcgaaggag ccaaattagg cttctgtttc cctgatctgg cactccaagg ggacacgccg
     1921 acagcgacag cagagacatg ctggaaaggt acaagctcat ccctggcaag cttcccacag
     1981 ctggactggg gctccgcgtt actgcaccca gaagttccat ggggggcgga gcccgactct
     2041 caggctcttc cgtggtccgg ggactggaca gacatggcgt gcacagcctg ggactcttgg
     2101 agcggcgcct cgcagaccct gggccccgcc cctctcggcc cgggccccat ccccgccgcc
     2161 ggctccgaag gcgccgcggg ccagaactgc gtccccgtgg cgggagaggc cacctcgtgg
     2221 tcgcgcgccc aggccgccgg gagcaacacc agctgggact gttctgtggg gcccgacggc
     2281 gatacctact ggggcagtgg cctgggcggg gagccgcgca cggactgtac catttcgtgg
     2341 ggcgggcccg cgggcccgga ctgtaccacc tcctggaacc cggggctgca tgcgggtggc
     2401 accacctctt tgaagcggta ccagagctca gctctcaccg tttgctccga accgagcccg
     2461 cagtcggacc gtgccagttt ggctcgatgc cccaaaacta accaccgagg tcccattcag
     2521 ctgtggcagt tcctcctgga gctgctccac gacggggcgc gtagcagctg catccgttgg
     2581 actggcaaca gccgcgagtt ccagctgtgc gaccccaaag aggtggctcg gctgtggggc
     2641 gagcgcaaga gaaagccggg catgaattac gagaagctga gccggggcct tcgctactac
     2701 tatcgccgcg acatcgtgcg caagagcggg gggcgaaagt acacgtaccg cttcgggggc
     2761 cgcgtgccca gcctagccta tccggactgt gcgggaggcg gacggggagc agagacacaa
     2821 taaaaccttg tacaaagtgg gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     2881 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     2941 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     3001 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     3061 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     3121 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     3181 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     3241 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     3301 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     3361 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     3421 ccacaaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     3481 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     3541 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     3601 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     3661 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     3721 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     3781 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     3841 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     3901 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     3961 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     4021 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     4081 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtgag
     4141 gtacccctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4201 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4261 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4321 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct cttcgaattt
     4381 aaatcggatc cagctgaatc taagtggatc tgcgatcgct ccggtgcccg tcagtgggca
     4441 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4501 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4561 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4621 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4681 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4741 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4801 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4861 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     4921 tacagatcta agctgtgacc ggcgcctact ctagagccgc catgtctaga ctggacaaga
     4981 gcaaagtcat aaactctgct ctggaattac tcaatggagt cggtatcgaa ggcctgacga
     5041 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     5101 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     5161 cctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     5221 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     5281 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     5341 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     5401 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     5461 ccccacttct gaaacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     5521 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     5581 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     5641 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     5701 ccggggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta
     5761 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     5821 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     5881 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5941 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6001 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6061 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6121 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6181 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6241 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6301 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     6361 atcaacctct ggattacaaa atttgtgggt aaacttgttt attgcagctt ataatggtta
     6421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6481 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg
     6541 tcaattagtc tatcagaagc tcatctggtc tcccttccgg gggacaagac atccctgttt
     6601 aatatttaaa cagcagtgtt cccaaactgg gttcttatat cccttgctct ggtcaaccag
     6661 gttgcagggt ttcctgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt
     6721 ttagccccgg aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt
     6781 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     6841 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     6901 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     6961 cgtaattaca tccctggggg ctttgggggg gggctgtccc tgatatctat aacaagaaaa
     7021 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     7081 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     7141 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     7201 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     7261 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc
     7321 tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     7381 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     7441 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     7501 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     7561 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     7621 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     7681 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     7741 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     7801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc at
//
