LOCUS       sequence-425305-        8646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for
            induction of global DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Hosseinpour M, Xi X, Liu L, Malaver-Ortega L, Perlaza-Jimenez L, Joo
            JE, York HM, Beesley J, Caldon CE, Dugue PA, Dowty JG, Arumugam S, 
            Southey MC, Rosenbluh J
  TITLE     SAM-DNMT3A, a strategy for induction of genome-wide DNA methylation,
            identifies DNA methylation as a vulnerability in ER-positive breast 
            cancers.
  JOURNAL   Nat Commun. 2024 Dec 1;15(1):10449. doi: 10.1038/s41467-024-54824-8.
  PUBMED    39617792
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-1"; volume: "15(1):10449. doi"; pages: " 
            10.1038/s41467-024-54824-8"
COMMENT     Sequence Label: SAM-DNMT3A-inactive+sgAAVS1_145
FEATURES             Location/Qualifiers
     source          1..8646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1717..1965
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1888..1907
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2233..2350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2399..2909
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2828..2846
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3374..3398)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3448..3468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4396..4449
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4477..5073
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4477..4496)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4973..4993
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5163..5396
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5474..5595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5511..5530)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5565..5584
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5635..5770
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5790..5809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5791..5809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5819..5836)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5819..5835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5828..5850)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5977..6432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6064..6083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6274..6295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6458..6562
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6563..6631,6632..7423)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6781..6800)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7594..8182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8083..8102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8336..8353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8470..8491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8506..8511,8512..8529,8530..8536)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8544..8560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8549..8571
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8568..8584
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8568..8584
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8603..8623
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8605..8623
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggga ctctttaagg
     1981 aaagagttta agagctatgc tgcgaatacg agggagcaga cgatatggcg tcgctcctct
     2041 ccacgagagc atatgggctc cgtggtctcg tacaccatca gggtacgtat cagacaccat
     2101 cagggtctgc tcgtattcgc agcatagcaa gtttaaataa ggctagtccg ttatcaactt
     2161 gaaaaagtgg caccgagtcg gtgctttttt tgaattctag atcttgagac aaatggcagt
     2221 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2281 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2341 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     2401 ggcccgggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     2461 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     2521 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     2581 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     2641 aagccgcacg tcttactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     2701 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     2761 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     2821 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     2881 ttgaccgaat caccgacctc tctccccagg gggatccaac ttaagcttgc caccatgtcg
     2941 cggaggcggc acagctacga gaacgatggt ggacaacctc acaaaaggag gaagacgtct
     3001 atgctagcct ccaaaaccat cgttctttcg gtcggcgagg ctactcgcac tctgactgag
     3061 atccagtcca ccgcagaccg tcagatcttc gaagagaagg tcgggcctct ggtgggtcgg
     3121 ctgcgcctca cggcttcgct ccgtcaaaac ggagccaaga ccgcgtatcg agtcaaccta
     3181 aaactggatc aggcggacgt cgttgattcc ggacttccga aagtgcgcta cactcaggta
     3241 tggtcgcacg acgtgacaat cgttgcgaat agcaccgagg cctcgcgcaa atcgttgtac
     3301 gatttgacca agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg
     3361 ccgctgggcc gttcttcaac tttcttgtac aaagtgggcg gaggaggtgg aagcggagga
     3421 ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctaac
     3481 cacgaccagg aatttgaccc tccaaaggtt tacccacctg tcccagctga gaagaggaag
     3541 cccatccggg tgctgtctct ctttgatgga atcgctacag ggctcctggt gctgaaggac
     3601 ttgggcattc aggtggaccg ctacattgcc tcggaggtgt gtgaggactc catcacggtg
     3661 ggcatggtgc ggcaccaggg gaagatcatg tacgtcgggg acgtccgcag cgtcacacag
     3721 aagcatatcc aggagtgggg cccattcgat ctggtgattg ggggcagtcc ctgcaatgac
     3781 ctctccatcg tcaaccctgc tcgcaagggc ctctacgagg gcactggccg gctcttcttt
     3841 gagttctacc gcctcctgca tgatgcgcgg cccaaggagg gagatgatcg ccccttcttc
     3901 tggctctttg ccaatgtggt ggccatgggc gttagtgaca agagggacat ctcgcgattt
     3961 ctcgagtcca accctgtgat gattgatgcc aaagaagtgt cagctgcaca cagggcccgc
     4021 tacttctggg gtaaccttcc cggtatgaac aggccgttgg catccactgt gaatgataag
     4081 ctggagctgc aggagtgtct ggagcatggc aggatagcca agttcagcaa agtgaggacc
     4141 attactacga ggtcaaactc cataaagcag ggcaaagacc agcattttcc tgtcttcatg
     4201 aatgagaaag aggacatctt atggtgcact gaaatggaaa gggtatttgg tttcccagtc
     4261 cactatactg acgtgtccaa catgagccgc ttggcgaggc agagactgct gggccggtca
     4321 tggagcgtgc cagtcatccg ccacctcttc gctccgctga aggagtattt tgcgtgtgtg
     4381 tgtacaggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     4441 cctggcccag gtaccacaga tccaccggag cttaccatga ccgagtacaa gcccacggtg
     4501 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     4561 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     4621 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     4681 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     4741 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     4801 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     4861 ggcgtttcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     4921 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     4981 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5041 cgcacctggt gcatgacccg caagcccggt gccgctagct gaacgcgtta agtcgacttt
     5101 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5161 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5221 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5281 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5341 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     5401 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5461 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5521 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5581 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5641 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5701 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5761 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     5821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5941 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6001 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6061 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6421 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6481 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6541 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6601 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6661 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6721 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6781 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6841 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6901 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6961 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7021 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7081 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7141 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7201 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7261 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7321 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7381 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7441 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7501 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7561 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7621 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7681 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7741 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7801 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7861 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7921 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7981 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8041 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8101 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8161 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8221 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8281 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8341 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8401 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8461 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8521 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8581 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8641 gcaagc
//
