LOCUS       sequence-425307-        8647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for
            induction of global DNA methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Hosseinpour M, Xi X, Liu L, Malaver-Ortega L, Perlaza-Jimenez L, Joo
            JE, York HM, Beesley J, Caldon CE, Dugue PA, Dowty JG, Arumugam S, 
            Southey MC, Rosenbluh J
  TITLE     SAM-DNMT3A, a strategy for induction of genome-wide DNA methylation,
            identifies DNA methylation as a vulnerability in ER-positive breast 
            cancers.
  JOURNAL   Nat Commun. 2024 Dec 1;15(1):10449. doi: 10.1038/s41467-024-54824-8.
  PUBMED    39617792
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-1"; volume: "15(1):10449. doi"; pages: " 
            10.1038/s41467-024-54824-8"
COMMENT     Sequence Label: SAM-DNMT3A-inactive+sgAAVS1_118
FEATURES             Location/Qualifiers
     source          1..8647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1717..1965
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1888..1907
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2234..2351
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2400..2910
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2829..2847
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(3375..3399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3449..3469
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4397..4450
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4478..5074
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4478..4497)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4974..4994
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5164..5397
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5475..5596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5512..5531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5566..5585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5636..5771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5791..5810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5792..5810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5820..5837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5820..5836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5829..5851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5978..6433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6065..6084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6275..6296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6459..6563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6564..6632,6633..7424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6782..6801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7595..8183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8084..8103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8337..8354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8471..8492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8507..8512,8513..8530,8531..8537)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8545..8561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8550..8572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8569..8585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8569..8585
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8604..8624
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8606..8624
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgccca ggccacctac
     1981 ttggccgttt aagagctatg ctgcgaatac gagggagcag acgatatggc gtcgctcctc
     2041 tccacgagag catatgggct ccgtggtctc gtacaccatc agggtacgta tcagacacca
     2101 tcagggtctg ctcgtattcg cagcatagca agtttaaata aggctagtcc gttatcaact
     2161 tgaaaaagtg gcaccgagtc ggtgcttttt ttgaattcta gatcttgaga caaatggcag
     2221 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2281 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2341 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     2401 gggcccgggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     2461 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     2521 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     2581 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     2641 gaagccgcac gtcttactag taccctcgca gacggacagc gccagggagc aatggcagcg
     2701 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     2761 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     2821 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     2881 gttgaccgaa tcaccgacct ctctccccag ggggatccaa cttaagcttg ccaccatgtc
     2941 gcggaggcgg cacagctacg agaacgatgg tggacaacct cacaaaagga ggaagacgtc
     3001 tatgctagcc tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga
     3061 gatccagtcc accgcagacc gtcagatctt cgaagagaag gtcgggcctc tggtgggtcg
     3121 gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gagtcaacct
     3181 aaaactggat caggcggacg tcgttgattc cggacttccg aaagtgcgct acactcaggt
     3241 atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta
     3301 cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa gatcttgtcg tcaaccttgt
     3361 gccgctgggc cgttcttcaa ctttcttgta caaagtgggc ggaggaggtg gaagcggagg
     3421 aggaggaagc ggaggaggag gtagcggacc taagaaaaag aggaaggtgg cggccgctaa
     3481 ccacgaccag gaatttgacc ctccaaaggt ttacccacct gtcccagctg agaagaggaa
     3541 gcccatccgg gtgctgtctc tctttgatgg aatcgctaca gggctcctgg tgctgaagga
     3601 cttgggcatt caggtggacc gctacattgc ctcggaggtg tgtgaggact ccatcacggt
     3661 gggcatggtg cggcaccagg ggaagatcat gtacgtcggg gacgtccgca gcgtcacaca
     3721 gaagcatatc caggagtggg gcccattcga tctggtgatt gggggcagtc cctgcaatga
     3781 cctctccatc gtcaaccctg ctcgcaaggg cctctacgag ggcactggcc ggctcttctt
     3841 tgagttctac cgcctcctgc atgatgcgcg gcccaaggag ggagatgatc gccccttctt
     3901 ctggctcttt gccaatgtgg tggccatggg cgttagtgac aagagggaca tctcgcgatt
     3961 tctcgagtcc aaccctgtga tgattgatgc caaagaagtg tcagctgcac acagggcccg
     4021 ctacttctgg ggtaaccttc ccggtatgaa caggccgttg gcatccactg tgaatgataa
     4081 gctggagctg caggagtgtc tggagcatgg caggatagcc aagttcagca aagtgaggac
     4141 cattactacg aggtcaaact ccataaagca gggcaaagac cagcattttc ctgtcttcat
     4201 gaatgagaaa gaggacatct tatggtgcac tgaaatggaa agggtatttg gtttcccagt
     4261 ccactatact gacgtgtcca acatgagccg cttggcgagg cagagactgc tgggccggtc
     4321 atggagcgtg ccagtcatcc gccacctctt cgctccgctg aaggagtatt ttgcgtgtgt
     4381 gtgtacaggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     4441 tcctggccca ggtaccacag atccaccgga gcttaccatg accgagtaca agcccacggt
     4501 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4561 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4621 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4681 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4741 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4801 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4861 cggcgtttcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4921 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4981 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5041 gcgcacctgg tgcatgaccc gcaagcccgg tgccgctagc tgaacgcgtt aagtcgactt
     5101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5161 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5401 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5461 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5581 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5701 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5761 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5821 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5881 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5941 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6421 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6481 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6541 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6601 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6661 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6721 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6781 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6841 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6901 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6961 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7021 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7081 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7141 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7201 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7261 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7321 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7381 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7441 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7501 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8461 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8521 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8581 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8641 tgcaagc
//
