LOCUS       sequence-42531-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J
  TITLE     Bridging the gap: a GFP-based strategy for overexpression and 
            purification of membrane proteins with intra and extracellular 
            C-termini.
  JOURNAL   Protein Sci. 2010 Apr;19(4):868-80.
  PUBMED    20196076
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2010-04"; volume: "19(4)"; pages: "868-80"
COMMENT     Sequence Label: pWarf(-)
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(101..121)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcttgtta gcagccggat ctcagtggtg gtggtggtga tgatgatggg ccgcaagctt
       61 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      121 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg
      181 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      241 tagttgaacg gaaccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt
      301 cttttgtttg tctgccgtga tgtatacatt gtgtgagtta tagttgtact ctagtttgtg
      361 tccgagaatg tttccatctt ctttaaaatc aatacccttt aactcgatac gattaacaag
      421 ggtatcacct tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga
      481 tatagtacgt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt
      541 catgtgatcc ggataacggg aaaagcattg aacaccatag gtcagagtag tgacaagtgt
      601 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtga gttttccgta
      661 tgtagcatca ccttcaccct ctccactgac agaaaattgt gcccattaac atcaccatct
      721 aattcaacaa gaatgggaca actccagtga aaagtcttcc c
//
