LOCUS       sequence-42532-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J
  TITLE     Bridging the gap: a GFP-based strategy for overexpression and 
            purification of membrane proteins with intra and extracellular 
            C-termini.
  JOURNAL   Protein Sci. 2010 Apr;19(4):868-80.
  PUBMED    20196076
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2010-04"; volume: "19(4)"; pages: "868-80"
COMMENT     Sequence Label: pWarf(+)
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..51)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(104..124)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcgggcttg ttagcagccg gatctcagtg gtggtggtgg tgatgatgat gggccgcaag
       61 ctttttgtag agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag
      121 gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta
      181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      241 tgctagttga acggaaccat cttcaatgtt gtggcgaatt ttgaagttag ctttgattcc
      301 attcttttgt ttgtctgccg tgatgtatac attgtgtgag ttatagttgt actctagttt
      361 gtgtccgaga atgtttccat cttctttaaa atcaataccc tttaactcga tacgattaac
      421 aagggtatca ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa
      481 agatatagtg cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      541 tttcatgtga tccggataac gggaaaagca ttgaacacca taggtcagag tagtgacaag
      601 tgttggccat ggaacaggta gttttccagt agtgcaaata
//
