LOCUS       sequence-425321-        9675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of EXT-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Solaimuthu B, Khatib A, Tanna M, Karmi A, Hayashi A, Abu Rmaileh A, 
            Lichtenstein M, Takoe S, Jolly MK, Shaul YD
  TITLE     The exostosin glycosyltransferase 1/STAT3 axis is a driver of breast
            cancer aggressiveness.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2316733121. doi: 
            10.1073/pnas.2316733121. Epub 2024 Jan 12.
  PUBMED    38215181
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-01-16"; volume: "121(3):e2316733121. 
            doi"; pages: " 10.1073/pnas.2316733121. Epub 2024 Jan 12"
COMMENT     Sequence Label: EXT1-pLJC2-3XFLAG
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2515..2580
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2628..2745
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2794..3304
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3223..3241
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3326..3925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3326..3345)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3822..3842
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4053..4286
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4358..4479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4395..4414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4449..4468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4519..4654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4674..4693)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4675..4693)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4703..4720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4703..4719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4712..4734)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4861..5316
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4948..4967)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5158..5179
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5342..5446
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5447..5515,5516..6307)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5665..5684)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6478..7066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6967..6986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7220..7237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7354..7375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7390..7395,7396..7413,7414..7420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7433..7455
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7452..7468
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7452..7468
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7487..7507
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7489..7507
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7535..7761
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7762..7942
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7989..8114
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8607..8840
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9025..9069
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9367..9387
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tttaattaag ccaccatgca ggccaaaaaa cgctatttca tcctgctctc agctggctct
      301 tgtctcgccc ttttgtttta tttcggaggc ttgcagttta gggcatcgag gagccacagc
      361 cggagagaag aacacagcgg taggaatggc ttgcaccacc ccagtccgga tcatttctgg
      421 ccccgcttcc cggacgctct gcgccccttc gttccttggg atcaattgga aaacgaggat
      481 tccagcgtgc acatttcccc ccggcagaag cgagatgcca actccagcat ctacaaaggc
      541 aagaagtgcc gcatggagtc ctgcttcgat ttcacccttt gcaagaaaaa cggcttcaaa
      601 gtctacgtat acccacagca aaaaggggag aaaatcgccg aaagttacca aaacattcta
      661 gcggccatcg agggctccag gttctacacc tcggacccca gccaggcgtg cctctttgtc
      721 ctgagtctgg atactttaga cagagaccag ttgtcacctc agtatgtgca caatttgaga
      781 tccaaagtgc agagtctcca cttgtggaac aatggtagga atcatttaat ttttaattta
      841 tattccggca cttggcctga ctacaccgag gacgtggggt ttgacatcgg ccaggcgatg
      901 ctggccaaag ccagcatcag tactgaaaac ttccgaccca actttgatgt ttctattccc
      961 ctcttttcta aggatcatcc caggacagga ggggagaggg ggtttttgaa gttcaacacc
     1021 atccctcctc tcaggaagta catgctggta ttcaagggga agaggtacct gacagggata
     1081 ggatcagaca ccaggaatgc cttatatcac gtccataacg gggaggacgt tgtgctcctc
     1141 accacctgca agcatggcaa agactggcaa aagcacaagg attctcgctg tgacagagac
     1201 aacaccgagt atgagaagta tgattatcgg gaaatgctgc acaatgccac tttctgtctg
     1261 gttcctcgtg gtcgcaggct tgggtccttc agattcctgg aggctttgca ggctgcctgc
     1321 gtccctgtga tgctcagcaa tggatgggag ttgccattct ctgaagtgat taattggaac
     1381 caagctgccg tcataggcga tgagagattg ttattacaga ttccttctac aatcaggtct
     1441 attcatcagg ataaaatcct agcacttaga cagcagacac aattcttgtg ggaggcttat
     1501 ttttcttcag ttgagaagat tgtattaact acactagaga ttattcagga cagaatattc
     1561 aagcacatat cacgtaacag tttaatatgg aacaaacatc ctggaggatt gttcgtacta
     1621 ccacagtatt catcttatct gggagatttt ccttactact atgctaattt aggtttaaag
     1681 cccccctcca aattcactgc agtcatccat gcggtgaccc ccctggtctc tcagtcccag
     1741 ccagtgttga agcttctcgt ggctgcagcc aagtcccagt actgtgccca gatcatagtt
     1801 ctatggaatt gtgacaagcc cctaccagcc aaacaccgct ggcctgccac tgctgtgcct
     1861 gtcgtcgtca ttgaaggaga gagcaaggtt atgagcagcc gttttctgcc ctacgacaac
     1921 atcatcacag acgccgtgct cagccttgac gaggacacgg tgctttcaac aacagaggtg
     1981 gatttcgcct tcacagtgtg gcagagcttc cctgagagga ttgtggggta ccccgcgcgc
     2041 agccacttct gggataactc taaggagcgg tggggataca catcaaagtg gacgaacgac
     2101 tactccatgg tgttgacagg agctgctatt taccacaaat attatcacta cctatactcc
     2161 cattacctgc cagccagcct gaagaacatg gtggaccaat tggccaattg tgaggacatt
     2221 ctcatgaact tcctggtgtc tgctgtgaca aaattgcctc caatcaaagt gacccagaag
     2281 aagcagtata aggagacaat gatgggacag acttctcggg cttcccgttg ggctgaccct
     2341 gaccactttg cccagcgaca gagctgcatg aatacgtttg ccagctggtt tggctacatg
     2401 ccgctgatcc actctcagat gaggctcgac cccgtcctct ttaaagacca ggtctctatt
     2461 ttgaggaaga aataccgaga cattgagcga cttggaggca gcgcggccgc aggcgactac
     2521 aaagaccatg acggtgatta taaagatcat gacattgatt acaaggatga cgatgacaag
     2581 taataagaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     2641 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2701 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2761 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
     2821 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     2881 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     2941 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     3001 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     3061 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     3121 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     3181 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     3241 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     3301 ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     3361 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3421 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3481 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3541 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3601 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3661 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     3721 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     3781 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     3841 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     3901 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
     3961 gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga
     4021 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4081 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     4321 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     4381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     4501 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4621 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     4681 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4741 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4801 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4861 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4921 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4981 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5041 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5101 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5161 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5221 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5281 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     5341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5461 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5521 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5581 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5641 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5701 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5761 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5821 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5881 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5941 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6001 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6061 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6121 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6181 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6241 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6301 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6601 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7141 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7201 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7261 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7321 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7381 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7441 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     7501 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     7561 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     7621 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     7681 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     7741 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     7801 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7861 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7921 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7981 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     8041 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     8101 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8161 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8221 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     8281 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8341 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     8401 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcagcagctg
     8461 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     8521 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     8581 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     8641 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     8701 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     8761 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8821 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8881 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8941 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9001 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9061 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9121 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9181 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     9241 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     9301 cgattagtga acggatctcg acggtatcga tcacgagact agcctcgagc agcagccccc
     9361 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     9421 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     9481 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     9541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9601 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9661 atcgctatta ccatg
//
