LOCUS       sequence-425325-       10631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium transformation. mCherry-sfGFP under the control of
            the cauliflower mosaic virus (CaMV) 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Ishikawa K, Kobayashi M, Kusano M, Numata K, Kodama Y
  TITLE     Using the organelle glue technique to engineer the plant cell 
            metabolome.
  JOURNAL   Plant Cell Rep. 2023 Mar;42(3):599-607. doi: 
            10.1007/s00299-023-02982-2. Epub 2023 Jan 27.
  PUBMED    36705704
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00299-023-02982-2"; journalName: "Plant Cell Rep"; date: 
            "2023-03"; volume: "42"; issue: "3"; pages: "599-607"
COMMENT     Sequence Label: pGWB602-mCherry-sfGFP
FEATURES             Location/Qualifiers
     source          1..10631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..696)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(748..927)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1433..1457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1978..2769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3015..3603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3504..3523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3757..3774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3789..3929
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3855..3874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4015..4037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4273..4467
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4533..5606)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6035..6664)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7965..7989
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8177..8199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8191..8208
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8192..8208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8723..9068
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9011..9031
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9090..9114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9138..9845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     9551..9570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(9861..9863,9864..9866,9867..10580)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9906..9927)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     10514..10535
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10591..10615)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacgtcc ccagctcggt accccctcct gggcacggtc gtacgcgatc cgccgcggtt
       61 ccacctcacc attgcgggta tgccagttga gttcgatgag ttcccgtacc agctccaact
      121 cgggcgatgc cacgcgcact ccgttcagat ctcggtgacg ggcaggaccg gacggggcgg
      181 taccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa
      241 gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct
      301 cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga
      361 cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac
      421 gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta
      481 ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag
      541 acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt
      601 gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc
      661 acggcggatg tcggccgggc gtcgttctgg gctcatatct tattgccccc cgggatcaga
      721 tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
      781 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
      841 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
      901 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
      961 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct
     1021 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     1081 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     1141 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     1201 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     1261 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     1321 gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc
     1381 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     1441 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     1501 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     1561 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     1621 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     1681 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1741 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1801 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1861 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1921 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1981 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2041 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2101 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2161 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2221 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2281 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2341 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2401 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2461 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2521 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2581 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2641 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2701 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2761 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     2821 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     2881 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     2941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3781 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3841 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3901 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3961 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4021 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     4081 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     4141 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     4201 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     4261 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     4321 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     4381 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     4441 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     4501 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     4561 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     4621 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     4681 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     4741 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     4801 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     4861 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     4921 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     4981 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     5041 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     5101 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     5161 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     5221 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     5281 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     5341 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     5401 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     5461 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     5521 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     5581 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     5641 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     5701 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     5761 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     5821 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     5881 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
     5941 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
     6001 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
     6061 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
     6121 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
     6181 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
     6241 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
     6301 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
     6361 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
     6421 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
     6481 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
     6541 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
     6601 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
     6661 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
     6721 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
     6781 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
     6841 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
     6901 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
     6961 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
     7021 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
     7081 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
     7141 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
     7201 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
     7261 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
     7321 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
     7381 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
     7441 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
     7501 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
     7561 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
     7621 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
     7681 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
     7741 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
     7801 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
     7861 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
     7921 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
     7981 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc
     8041 aagctgctct agcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8101 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8161 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca
     8221 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
     8281 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
     8341 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
     8401 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
     8461 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
     8521 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
     8581 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
     8641 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
     8701 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
     8761 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
     8821 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
     8881 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     8941 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     9001 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     9061 agagaacacg ggggactcta gagttatcaa caagtttgta caaaaaagca ggctttaaag
     9121 gaaccaattc agtcgacatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     9181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     9241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     9301 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     9361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     9421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     9481 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     9541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     9601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     9661 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     9721 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     9781 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     9841 acaagggagg ctctggaggc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     9901 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg agaggcgagg
     9961 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
    10021 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
    10081 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
    10141 tccaggagcg caccatctct ttcaaggacg acggcactta caagacccgc gccgaggtga
    10201 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
    10261 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac gtctatatca
    10321 ctgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacgttgagg
    10381 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
    10441 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
    10501 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
    10561 tggacgagct gtacaagtaa ggatatctag acccagcttt cttgtacaaa gtggttgata
    10621 acagcgctta g
//
