LOCUS       sequence-425330-       10507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of mCherry flanked with an 
            N-terminal plastid transit peptide in plants (for 
            Agrobacterium-mediated genetic transformation and visualization of 
            plastid.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Ishikawa K, Kobayashi M, Kusano M, Numata K, Kodama Y
  TITLE     Using the organelle glue technique to engineer the plant cell 
            metabolome.
  JOURNAL   Plant Cell Rep. 2023 Mar;42(3):599-607. doi: 
            10.1007/s00299-023-02982-2. Epub 2023 Jan 27.
  PUBMED    36705704
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00299-023-02982-2"; journalName: "Plant Cell Rep"; date: 
            "2023-03"; volume: "42"; issue: "3"; pages: "599-607"
COMMENT     Sequence Label: pGWB508-cTP-mCherry
FEATURES             Location/Qualifiers
     source          1..10507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1356..2063
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1769..1788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2065..2089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2100..2117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2267..3292)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3344..3523)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4029..4053
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4574..5365
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5611..6199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6100..6119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6353..6370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6385..6525
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6451..6470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6611..6633
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6869..7063
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7129..8202)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8631..9260)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctccatggg aaaaacttcg ggagcgaaac aggcgactgt ggtggtcgca gcgatggcgt
     1261 taggatggtt agccatagag atcgctttca agcctttcct cgataaattc cgctcctcaa
     1321 tcgacaaatc tgacccaacc aaagaccccg atgacatggt gagcaagggc gaggaggata
     1381 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1441 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1501 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1561 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1621 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1681 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1741 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1801 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1861 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1921 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1981 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2041 gcggcatgga cgagctgtac aaggacccag ctttcttgta caaagtggtt gataacagcc
     2101 atcatcatca tcatcatgct tagagctggg gatcgatccc gcgaactgtg gacgagaact
     2161 gtgccaccaa gcgtaaggcc gttctctcgc atttgccttg ctaggctcgc gcgagttgct
     2221 ggctgaggcg ttctcgaaat cagctcttgt tcggtcggca tctactctat tcctttgccc
     2281 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     2341 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     2401 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     2461 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     2521 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     2581 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     2641 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     2701 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     2761 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     2821 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     2881 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     2941 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     3001 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaatgcc ccaatgtcaa
     3061 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     3121 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     3181 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     3241 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcttatt
     3301 gccccccggg atcagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg
     3361 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     3421 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     3481 tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta
     3541 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc
     3601 atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3661 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     3721 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     3781 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     3841 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     3901 ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt aatagaaata aattcagcct
     3961 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     4021 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     4081 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     4141 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     4201 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     4261 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     4321 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     4381 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     4441 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     4501 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     4561 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     4621 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     4681 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     4741 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     4801 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     4861 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     4921 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     4981 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     5041 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     5101 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     5161 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     5221 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     5281 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     5341 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     5401 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     5461 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     5521 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6361 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6421 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6481 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     6541 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6601 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6661 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     6721 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     6781 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     6841 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     6901 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     6961 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     7021 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     7081 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     7141 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     7201 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     7261 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     7321 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     7381 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     7441 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     7501 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     7561 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     7621 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     7681 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     7741 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     7801 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     7861 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     7921 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     7981 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     8041 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     8101 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     8161 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
     8221 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
     8281 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
     8341 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
     8401 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
     8461 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
     8521 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
     8581 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
     8641 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
     8701 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
     8761 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
     8821 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
     8881 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
     8941 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
     9001 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
     9061 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
     9121 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
     9181 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
     9241 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
     9301 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
     9361 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
     9421 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
     9481 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
     9541 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
     9601 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
     9661 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
     9721 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
     9781 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
     9841 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
     9901 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
     9961 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    10021 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    10081 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    10141 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    10201 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    10261 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    10321 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    10381 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    10441 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    10501 taaacct
//
