LOCUS       sequence-425331-       10564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of sfGFP flanked with an N-terminal
            plastid transit peptide in plants (for Agrobacterium-mediated 
            genetic transformation and visualization of plastid.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Ishikawa K, Kobayashi M, Kusano M, Numata K, Kodama Y
  TITLE     Using the organelle glue technique to engineer the plant cell 
            metabolome.
  JOURNAL   Plant Cell Rep. 2023 Mar;42(3):599-607. doi: 
            10.1007/s00299-023-02982-2. Epub 2023 Jan 27.
  PUBMED    36705704
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00299-023-02982-2"; journalName: "Plant Cell Rep"; date: 
            "2023-03"; volume: "42"; issue: "3"; pages: "599-607"
COMMENT     Sequence Label: pGWB508-cTP-sfGFP
FEATURES             Location/Qualifiers
     source          1..10564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1392..1394,1395..1397,1398..2111)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1437..1458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2045..2066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2122..2146)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2157..2174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2324..3349)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3401..3580)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4086..4110
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4631..5422
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5668..6256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6157..6176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6410..6427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6442..6582
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6508..6527)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6668..6690
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6926..7120
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7186..8259)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8688..9317)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg gaaaaacttc gggagcgaaa caggcgactg
     1261 tggtggtcgc agcgatggcg ttaggatggt tagccataga gatcgctttc aagcctttcc
     1321 tcgataaatt ccgctcctca atcgacaaat ctgacccaac caaagacccc gatgacggcg
     1381 gtagcggcgg tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1441 tcgagctgga cggcgacgta aacggccaca agttcagcgt gagaggcgag ggcgagggcg
     1501 atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1561 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1621 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1681 gcaccatctc tttcaaggac gacggcactt acaagacccg cgccgaggtg aagttcgagg
     1741 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1801 tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc actgccgaca
     1861 agcagaagaa cggcatcaag gccaacttca agatccgcca caacgttgag gacggcagcg
     1921 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1981 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     2041 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2101 tgtacaagta aggatatcta gacccagctt tcttgtacaa agtggttgat aacagccatc
     2161 atcatcatca tcatgcttag agctggggat cgatcccgcg aactgtggac gagaactgtg
     2221 ccaccaagcg taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc
     2281 tgaggcgttc tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg
     2341 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     2401 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     2461 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     2521 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     2581 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     2641 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     2701 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     2761 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     2821 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     2881 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     2941 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     3001 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     3061 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaatgcccca atgtcaagca
     3121 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     3181 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     3241 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     3301 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcttattgcc
     3361 ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
     3421 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     3481 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     3541 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     3601 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     3661 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     3721 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     3781 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     3841 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     3901 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     3961 agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat tcagcctaat
     4021 tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     4081 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     4141 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     4201 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     4261 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     4321 gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg
     4381 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     4441 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     4501 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     4561 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     4621 gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     4681 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     4741 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     4801 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     4861 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4921 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4981 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     5041 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     5101 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     5161 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     5221 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     5281 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     5341 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     5401 caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg
     5461 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     5521 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     5581 agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6421 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6481 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6541 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     6601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6721 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     6781 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     6841 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     6901 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     6961 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     7021 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     7081 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     7141 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     7201 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     7261 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     7321 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     7381 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     7441 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     7501 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     7561 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     7621 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     7681 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     7741 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     7801 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     7861 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     7921 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     7981 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     8041 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     8101 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     8161 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     8221 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     8281 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
     8341 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     8401 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     8461 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     8521 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     8581 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     8641 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     8701 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     8761 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     8821 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     8881 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     8941 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     9001 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     9061 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     9121 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     9181 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     9241 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     9301 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     9361 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     9421 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     9481 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     9541 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     9601 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     9661 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     9721 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     9781 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     9841 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     9901 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     9961 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    10021 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    10081 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    10141 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    10201 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    10261 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    10321 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    10381 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    10441 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    10501 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    10561 acct
//
